ISSN:
1432-1017
Keywords:
Key words Analytical ultracentrifugation
;
Self-association
;
Heterologous association
;
Protein protein interactions
;
Protein nucleic acid interaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Many of the functions of biological macromolecules are based on specific interactions. Extended concentration dependent studies of sedimentation coefficients or molecular masses of biopolymers are highly useful for describing the different kinds of association phenomena. These studies allow one to determine the partial concentrations of monomers and associates or reactants and complexes in self-associating systems or heterologous associations, respectively. Furthermore, in combination with corresponding measurements of biological activity these data allow one to estimate the individual activity parameters of components involved in equilibrium processes. The study of self-association and heterologous association using analytical ultracentrifugation, some recent developments therein, and its application to different examples are outlined here.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002490050046
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