ISSN:
1040-0397
Keywords:
Glucose
;
Glucose oxidase
;
Poly(p-aminophenol)
;
Enzyme electrode
;
Amperometric biosensor
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An amperometric biosensor for glucose based on a novel polymeric matrix is described. The sensor was prepared by electrochemical polymerization of p-aminophenol in the presence of glucose oxidase on Pt substrates in a KCl solution at a potential of 0.6 V (vs. Ag/AgCl). The chronoamperometric response of poly(p-aminopheno1)-glucose oxidase biosensor to hydrogen peroxide formed as the result of enzymatic reaction was measured at a potential of 0.7 V (vs. Ag/AgCl) in PBS solution. It was shown that a biosensor prepared by this one- step procedure has a linear range up to 6mM glucose, a response time less than 5 s and a lifetime around 15 days. The sensitivity of the electrode was superior to that of much publicized polypyrrole based glucose oxidase electrode. Also, the substrate selectivity of PAP/GOx electrode was tested for coexisting substances such as lactose, sucrose, urea, paracetamol, uric and ascorbic acid and no discernible anodic response was observed above the background current.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140080613
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