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  • 1
    Electronic Resource
    Electronic Resource
    A Method for Isolation of Total RNA from Fruit Tissues of Banana (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 87-87 
    Details
    ISSN: 1572-9818
    Keywords: banana ; fruit tissue ; Musa sp. ; RNA isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007492421119
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  • 2
    Electronic Resource
    Electronic Resource
    An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture (1995)
    Springer
    Plant cell reports 14 (1995), S. 510-514 
    Details
    ISSN: 1432-203X
    Keywords: Aranda Deborah ; orchids ; thin section culture ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A thin section culture system for rapid regeneration of the monopodial orchid hybrid Aranda Deborah has been developed. Thin sections (0.6–0.7mm thick) obtained by transverse sectioning of a single shoot tip (6–7mm), when cultured in Vacin and Went medium enriched with coconut water (20% v/v), produced an average 13.6 protocorm-like bodies (PLB) after 45 days, compared to 2.7 PLB formed by a single 6–7 mm long shoot tip under same culture condition. Addition of α-naphthaleneacetic acid to Vacin and Went medium enriched with coconut water further increased PLB production by thin sections. PLB developed into plantlets on solid Vacin and Went medium containing 10% (v/v) coconut water and 0.5 g l−1 activated charcoal. With this procedure, more than 80,000 plantlets could be produced from thin sections obtained from a single shoot tip in a year as compared to nearly 11,000 plantlets produced by the conventional shoot tip method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232785
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  • 3
    Electronic Resource
    Electronic Resource
    High frequency adventitious shoot regeneration from excised leaves of Paulownia spp. cultured in vitro (1996)
    Springer
    Plant cell reports 16 (1996), S. 204-209 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. High frequency, direct regeneration of shoots was induced in leaf cultures of Paulownia tomentosa, P. fortunei × P. tomentosa and P. kawakamii. The optimum culture medium for the leaf explants derived from shoot cultures was Murashige-Skoog (MS) medium supplemented with 10 μM indole-3-acetic acid and 50 μM benzyladenine. Up to 40 shoots were obtained over a 4 month culture period from each leaf explant. Rooting occurred spontaneously in the shoots that were about 1 cm tall when subcultured on phytohormone-free MS medium. The plantlets could be transplanted successfully. Some of the transplanted P. tomentosa plantlets flowered in the greenhouse one year after transplanting. The protocol is suitable not only for rapid multiplication of the various species of Paulownia, but also for analytical studies associated with adventitious shoot regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050207
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  • 4
    Electronic Resource
    Electronic Resource
    High frequency adventitious shoot regeneration from excised leaves ofPaulownia spp. cultured in vitro (1996)
    Springer
    Plant cell reports 16 (1996), S. 204-209 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract High frequency, direct regeneration of shoots was induced in leaf cultures ofPaulownia tomentosa, P. fortunei x P. tomentosa andP. kawakamii. The optimum culture medium for the leaf explants derived from shoot cultures was Murashige-Skoog (MS) medium supplemented with 10 μM indole-3-acetic acid and 50 μM benzyladenine. Up to 40 shoots were obtained over a 4 month culture period from each leaf explant. Rooting occurred spontaneously in the shoots that were about 1 cm tall when subcultured on phytohormone-free MS medium. The plantlets could be transplanted successfully. Some of the transplantedP. tomentosa plantlets flowered in the greenhouse one year after transplanting. The protocol is suitable not only for rapid multiplication of the various species ofPaulownia, but also for analytical studies associated with adventitious shoot regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01890868
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  • 5
    Electronic Resource
    Electronic Resource
    Ethylene and CO2 affect direct shoot regeneration from the petiolar ends of Paulownia kawakamii leaves cultured in vitro (1996)
    Springer
    Plant growth regulation 20 (1996), S. 237-243 
    Details
    ISSN: 1573-5087
    Keywords: callus ; carbon dioxide ; ethylene ; leaf culture ; Paulownia kawakamii ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of ethylene and CO2 on shoot regeneration in excised leaf cultures of Paulownia kawakamii were examined. When both the gases were prevented from accumulating in the headspace of cultures using mercuric perchlorate and potassium hydroxide traps, shoot regeneration frequency improved and callus production was reduced compared to the control and cultures with only one of the gases trapped. Incorporation of either aminoethoxyvinylglycine (AVG) or 1-amino-cyclopropane-1-carboxylic acid (ACC) in the culture medium caused significant reduction in shoot regeneration. There was profuse callus production in the presence of high amounts of ACC, which was accompanied by over sixfold increase in the rate of ethylene production. However, in the presence of AVG callus production was delayed and shoot regeneration decreased, suggesting that low levels of ethylene might be needed for de novo shoot bud induction in Paulownia cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043313
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  • 6
    Electronic Resource
    Electronic Resource
    Involvement of ethylene on growth and plant regeneration in callus cultures of Heliconia psittacorum L.f. (1996)
    Springer
    Plant growth regulation 19 (1996), S. 145-151 
    Details
    ISSN: 1573-5087
    Keywords: ethylene inhibitors ; headspace gas ; Heliconia ; morphogenesis ; protocorm-like bodies ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The level of ethylene accumulated in morphogenic callus cultures of Heliconia psittacorum L.f. was only one quarter that of non-morphogenic cultures. The rate of ethylene production in the morphogenic callus cultures during early stages of differentiation of protocorm-like bodies leading to plantlet regeneration was 10-fold higher than that during callus proliferation. In cultures sealed with gastight serum caps, fresh weight gain was reduced 2-to 3-fold compared to those that were closed with Kaputsℜ. Treatment with 1-aminocyclopropane-1-carboxylic acid (≥ 100 μM) caused complete inhibition of plant regeneration from the morphogenic callus on subsequent culture under inductive conditions. Silver nitrate and aminoethoxyvinylglycine also reduced plant regeneration. These results indicate that while high levels of ethylene were inhibitory, a low level of endogenous ethylene production may be necessary during the plant regeneration phase in callus cultures of Heliconia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024581
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  • 7
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    The complete sequence of a Singapore isolate of odontoglossum ringspot virus and comparison with other tobamoviruses (1996)
    Elsevier
    Details
    Publication Date: 1996-06-01
    Print ISSN: 0378-1119
    Electronic ISSN: 1879-0038
    Topics: Biology
    Published by Elsevier
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