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  • 1
    Electronic Resource
    Electronic Resource
    Plant protein secretion on tap (1999)
    [s.l.] : Nature America Inc.
    Nature biotechnology 17 (1999), S. 427-427 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Transgenic plants have shown tremendous potential as photosynthetic bioreactors for the production of foreign proteins such as antibodies and streptavidin. But harvesting these recombinant molecules can be problematic because they typically are sequestered inside plant cells and require tissue ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/8594
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation of 12 different plasmids into soybean via particle bombardment (1996)
    Springer
    Plant cell reports 15 (1996), S. 500-505 
    Details
    ISSN: 1432-203X
    Keywords: Glycine max ; Recombination ; Soybean ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Particle bombardment offers a simple method for the introduction of DNA into plant cells. Multiple DNA fragments may be introduced on a single plasmid or on separate plasmids (co-transformation). To investigate some of the properties and limits of co-transformation, 12 different plasmids were introduced into embryogenic suspension culture tissue of soybean [Glycine max (L.) Merrill] via particle bombardment. The DNAs used for co-transformation included 10 plasmids containing KFLP markers for maize and 2 plasmids separately encoding hygromycin-resistance and ß-glucuronidase. Two weeks following bombardment with the 12 different plasmids, suspension culture tissue was placed under hygromycin selection. Hygromycin-resistant clones were isolated after an additional 5 to 6 weeks. Southern hybridization analysis of 26 hygromycin-resistant embryogenic clones verified the presence of introduced plasmid DNAs. All of the co-transforming plasmids were present in most of the transgenic soybean clones and there was no preferential uptake and integration of any of the plasmids. The copy number of individual plasmids was approximately equal within clones but highly variable between clones. While some clones contained as few as zero to three copies of each plasmid, others clones contained as many as 10 to 15 copies of each of the 12 different plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232982
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  • 3
    Electronic Resource
    Electronic Resource
    Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.) (1996)
    Springer
    Plant cell reports 15 (1996), S. 489-494 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report a strength comparison of a large variety of monocot and dicot intron-containing fragments inserted in the 5′ untranslated leader, between the CaMV 35S promoter and the uidA gene (coding for the ß-glucuronidase: GUS). Relative strengths of the intron-containing fragments were evaluated by comparing transient GUS expression after particle bombardment in embryogenic maize and bluegrass suspension cultures. Our results confirm a dramatic dependence on the presence of an intron for chimeric gene expression in both species. On average, the maize first intron of ubi1 provided the highest enhancement of gene expression in maize and bluegrass (71- and 26-fold enhancement, respectively). Half of the introns tested affected gene expression differently in bluegrass and maize. This suggests that the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots. We also report enhancement of gene expression (92-fold) in a monocot species by a dicot intron (chsA intron).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232980
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  • 4
    Electronic Resource
    Electronic Resource
    Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.) (1996)
    Springer
    Plant cell reports 15 (1996), S. 489-494 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We report a strength comparison of a large variety of monocot and dicot intron-containing fragments inserted in the 5' untranslated leader, between the CaMV 35S promoter and the uidA gene (coding for the β-glucuronidase: GUS). Relative strengths of the intron-containing fragments were evaluated by comparing transient GUS expression after particle bombardment in embryogenic maize and bluegrass suspension cultures. Our results confirm a dramatic dependence on the presence of an intron for chimeric gene expression in both species. On average, the maize first intron of ubil provided the highest enhancement of gene expression in maize and bluegrass (71- and 26-fold enhancement, respectively). Half of the introns tested affected gene expression differently in bluegrass and maize. This suggests that the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots. We also report enhancement of gene expression (92-fold) in a monocot species by a dicot intron (chsA intron).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050060
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  • 5
    Electronic Resource
    Electronic Resource
    Transformation of 12 different plasmids into soybean via particle bombardment (1996)
    Springer
    Plant cell reports 15 (1996), S. 500-505 
    Details
    ISSN: 1432-203X
    Keywords: Key Words:Glycine max– Recombination – Soybean – Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Particle bombardment offers a simple method for the introduction of DNA into plant cells. Multiple DNA fragments may be introduced on a single plasmid or on separate plasmids (co-transformation). To investigate some of the properties and limits of co-transformation, 12 different plasmids were introduced into embryogenic suspension culture tissue of soybean [Glycine max (L.) Merrill] via particle bombardment. The DNAs used for co-transformation included 10 plasmids containing RFLP markers for maize and 2 plasmids separately encoding hygromycin-resistance and β-glucuronidase. Two weeks following bombardment with the 12 different plasmids, suspension culture tissue was placed under hygromycin selection. Hygromycin-resistant clones were isolated after an additional 5 to 6 weeks. Southern hybridization analysis of 26 hygromycin-resistant embryogenic clones verified the presence of introduced plasmid DNAs. All of the co-transforming plasmids were present in most of the transgenic soybean clones and there was no preferential uptake and integration of any of the plasmids. The copy number of individual plasmids was approximately equal within clones but highly variable between clones. While some clones contained as few as zero to three copies of each plasmid, others clones contained as many as 10 to 15 copies of each of the 12 different plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050062
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  • 6
    Electronic Resource
    Electronic Resource
    SAAT: sonication-assisted Agrobacterium-mediated transformation (1997)
    Springer
    Transgenic research 6 (1997), S. 329-336 
    Details
    ISSN: 1573-9368
    Keywords: Agrobacterium ; SAAT ; sonication ; transformation ; wounding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant transformation via Agrobacterium can be limited by both host specificity and the inability of Agrobacterium to reach the proper cells in the target tissue. Described here is a new and efficient Agrobacterium-based transformation technology that overcomes these barriers and enhances DNA transfer in such diverse plant groups as dicots, monocots, and gymnosperms. This new technology, called sonication-assisted Agrobacterium-mediated transformation (SAAT), involves subjecting the plant tissue to brief periods of ultrasound in the presence of Agrobacterium. Scanning electron and light microscopy reveal that SAAT treatment produces small and uniform fissures and channels throughout the tissue allowing the Agrobacterium easy access to internal plant tissues. Unlike other transformation methods, this system has the potential to transform meristematic tissue buried under several cell layers. SAAT increases transient transformation efficiency in several different plant tissues including leaf tissue, immature cotyledons, somatic and zygotic embryos, roots, stems, shoot apices, embryogenic suspension cells and whole seedlings. A 100- to 1400-fold increase in transient β- glucuronid ase expression has been demonstrated in various tissues of soybean, Ohio buckeye, cowpea, white spruce, wheat and maize. Stable transformation of both soybean and Ohio buckeye has been obtained using SAAT of embryogenic suspension culture tissues. For soybean, SAAT treatment was necessary to obtain stable transformation with this tissue
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018470930944
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  • 7
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    Plant protein secretion on tap (1999)
    Springer Nature
    Details
    Publication Date: 1999-05-01
    Print ISSN: 1087-0156
    Electronic ISSN: 1546-1696
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer Nature
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