ISSN:
1432-2145
Keywords:
Key words Helianthus annuus L.
;
Mature free pollen
;
Zinc finger
;
DNA binding
;
RNA binding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The protein PLIM-1 (formerly SF3) from sunflower is expressed exclusively in mature, free pollen. It contains two LIM domains associated with an acidic C-terminus comprising six copies of the pentapeptide motif (A,T,S) (E,D) TQN. We have expressed the pollen protein as well as some of its mutant forms in Escherichia coli and have used the bacterially produced proteins to study interactions with nucleic acids. Our studies show that the protein binds DNA and RNA in vitro to form large complexes, while mutant polypeptides containing either a single LIM domain or a destabilized first or second LIM domain do not. Although these data suggest that the biological function of PLIM-1 involves interactions with nucleic acids, its role in pollen development remains unclear.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004970050041
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