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  • 1
    Electronic Resource
    Electronic Resource
    75th birthday of Professor Jaroslav Janák (1999)
    Springer
    Chromatographia 49 (1999), S. 479-479 
    Details
    ISSN: 1612-1112
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02467744
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  • 2
    Electronic Resource
    Electronic Resource
    An improvement of restriction analysis of bacteriophage DNA using capillary electrophoresis in agarose solution (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 366-376 
    Details
    ISSN: 0173-0835
    Keywords: Capillary electrophoresis ; Genomic DNA ; Bacteriophage ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Seven representatives of the serogroup B Staphylococcus aureus bacteriophages, 29, 53, 55, 83A, 85, φ11 and 80α, were examined by capillary electrophoresis (CE) for genomic homology using DNA restriction analysis. Genomic DNA of individual bacteriophages was cleaved by HindIII restriction endonuclease, and the resulting restriction fragments were separated by standard horizontal agarose slab gel electrophoresis (SGE) as well as by CE in low-melting-point agarose solutions. The number and size of restriction fragments identified by both methods were compared. The high separation power of CE makes it possible to extend the restriction fragment patterns. In most of the restriction patterns, some additional restriction fragments as small as 150 bp, not identified by SGE, were detected. With respect to speed, high separation efficiency, low sample consumption and automation, CE offers a simple procedure for processing of multiple samples cost-effectively in a reasonable time. The comparison of the complemented restriction patterns of the different phage strains and the subsequent identification of their common fragments leads to a deeper understanding of their phylogenetic relationships. The genome homologies expressed for individual phage pairs in terms of coefficient F values ranged from 15 to 69%. These values are in good accordance with the degree of DNA homology of these phages as determined by DNA hybridization studies and thermal denaturation analysis of DNA by other authors. The total size of each phage genome was estimated by adding the sizes of individual restriction fragments.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150160163
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  • 3
    Electronic Resource
    Electronic Resource
    Advances in chromatography, volume 34 editors: Phyllis R. Brown and Eli Grushka Marcell Dekker, Inc. New York 1994, pp. I-XVI, 1-435 ISBN: 0-8247-9087-1, Price: $ 165.00 (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1074-1074 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601178
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  • 4
    Electronic Resource
    Electronic Resource
    Application of capillary zone electrophoresis for analysis of thyreostatics (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 1959-1963 
    Details
    ISSN: 0173-0835
    Keywords: Capillary zone electrophoresis ; Thyreostatics ; Methylthiouracil ; Urine ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Capillary zone electrophoresis was optimized for the separation of thiouracil, methylthiouracil and propylthiouracil. Methylthiouracil could be determined in various types of urine (human, bovine, horse), either without any pretreatment or in ethyl acetate extracts, within 15 min. For identification, the simultaneous detection at three UV wavelengths (216, 245 and 278 nm) was advantageously used while for quantification the wavelength of the absorbance maximum at 278 nm was preferred. Under optimized conditions a linear response of the detector in the concentration range 0.1-100 ppm was obtained. On analysis of untreated urine, a detection limit of 0.5 ppm was found; for urine extracts the detection limit was 0.1 ppm. Univocal peak identification, based on absorption at three wavelength, was only possible above 2 ppm. Relative standard deviation for standard solutions of methylthiouracil, diluted in the background electrolyte, was 1%; for methylthiouracil in extracts dissolved in the background electrolyte it was 4.5%, and for methylthiouracil in untreated urine, 12.7%.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150171226
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  • 5
    Electronic Resource
    Electronic Resource
    Recent application and developments of capillary isotachophoresis (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 2154-2161 
    Details
    ISSN: 0173-0835
    Keywords: Isotachophoresis ; Capillary zone electrophoresis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Capillary isotachophoresis is a powerful electromigration separation method with a pronounced capability to concentrate trace components in diluted samples. At present, capillary isotachophoresis is utilized predominantly as the first step in on-line combination with capillary zone electrophoresis. This article is a continuation of previous reviews and summarizes the results published during 1993-1996.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150181206
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  • 6
    Electronic Resource
    Electronic Resource
    Interval isotachophoresis for purification and isolation of ionogenic species (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 3090-3093 
    Details
    ISSN: 0173-0835
    Keywords: Preparative electrophoresis ; Interval preparative isotachophoresis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A new preparative electrophoretic method in free solution is described, consisting of three consecutive steps: (i) filling the separation chamber with a suitable electrolyte system and sample in parallel streams by laminar hydrodynamic flow, (ii) applying the voltage across the chamber with isotachophoretic separation for a definite time interval operating in the direction perpendicular to that of filling, (iii) reapplying a hydrodynamic flow (without voltage) and collecting the separated species via an array of outlets. This approach completely eliminates the main drawback of the continuous flow electrophoresis (CFE), i.e., the electrohydrodynamic distortion of zones. This method utilizes the instrumentation devised for CFE and enables the isolation of large amounts of individual sample species comparable to that of CFE, with a resolution comparable to that of capillary isotachophoresis. The precise timing of the consecutive steps in the procedure as well as the stability of the operational parameters are of key importance for reproducibility. By using cationic isotachophoresis with 3 synthetic pI markers as model sample species, the reproducibility, stability and the separation power of the newly presented method are demonstrated. The sample throughput corresponds to micromoles per hour.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191808
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of heparin-like pharmaceuticals by capillary zone electrophoresis and isotachophoresis (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 125-129 
    Details
    ISSN: 0173-0835
    Keywords: Capillary zone electrophoresis ; Isotachophoresis ; Heparinlike pharmaceuticals ; Aprosulate ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Synthetic sulfated bis-lactobionic acid amides are important heparin-like pharmaceuticals. The synthesis of these compounds yields molecules differing in the number of sulfate groups, and during the isolation procedure, the required species may partially decompose or take in some impurities. This article shows that capillary zone electrophoresis may serve well as an expedient method for the analysis of the above-mentioned pharmaceuticals. Complex-forming equilibria between the analytes and bivalent cations present in the background electrolyte bring selectivity necessary for the separation, and the detection at various wavelengths serves as an aid in the characterization of admixtures, decomposition products, and impurities. Capillary isotachophoresis may also be used for the analysis of these species, bringing about the potential of micropreparation of individual compounds and opening the chance for continuous free-flow electrophoresis.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150170121
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  • 8
    Electronic Resource
    Electronic Resource
    A comparison of resolution of DNA fragments between agarose gel and capillary zone electrophoresis in agarose solutions (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1345-1353 
    Details
    ISSN: 0173-0835
    Keywords: Resolution ; DNA ; Capillary zone electrophoresis ; Gel electrophoresis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The resolving power of capillary zone electrophoresis (CZE) is compared to that of gel electrophoresis (GE) under similar conditions (agarose, similar length of DNA fragments, identical buffer) but with differences in temperature and field strength. The comparison is based on the time required to reach a desired degree of resolution by each of the two methods. A resolution parameter is developed which is equally applicable to CZE, with relatively diffuse initial conditions in the absence of stacking and measurements expressed in terms of time, and to GE, in which measurements are expressed in terms of spatial parameters. The resolution time in CZE using agarose solutions at 40°C was found to be greater by at least one order of magnitude than that in GE using agarose gels. Thus, the increased migration velocity due to high field strength in CZE substantially outweights the lower dispersion in GE.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601222
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  • 9
    Electronic Resource
    Electronic Resource
    A practical procedure for the determination of association constants of the analyte-chiral selector equilibria by capilllary zone electrophoresis (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 1921-1924 
    Details
    ISSN: 0173-0835
    Keywords: Capillary zone electrophoresis ; Chiral separations ; Association constants ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A practical procedure is proposed for the determination of association constants and mobility of the associate of a solute with a chiral selector in chiral separations by capillary zone electrophoresis. The procedure is based on the measurement of the effective mobility of a solute at zero and two different nonzero concentrations of the chiral selector. Simple explicit formulas have been derived in order to calculate the required data. The essence of the procedure is that all mobility data are adjusted to the background electrolyte (BGE) without chiral selector, serving as the viscosity reference. A simple procedure is described for measuring the viscosity of the operational electrolytes directly with the commercial capillary electrophoresis instrumentation, and Walden's rule has been utilized for adjusting the experimental mobility data to constant reference viscosity. The use of the procedure is exemplified by a separation of D,L-tryptophan in BGE containing α-cyclodextrin as chiral selector.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150171219
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  • 10
    Electronic Resource
    Electronic Resource
    Theory of zone separation in isotachophoresis: A diffusional approach (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1999-2007 
    Details
    ISSN: 0173-0835
    Keywords: Isotachophoresis ; Zone electrophoresis ; Separation theory ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The qualitative characteristics of isotachophoresis differ substantially from “classical” separation methods such as chromatography or zone electrophoresis. Self-sharpening zone boundaries and step-like concentration profiles are the most specific features of this method, which does not allow the description of the isotachophoretic separation in usual “chromatographic” terms, such as resolution or number of theoretical plates. A theory is presented in this paper, combining the usual isotachophoretic separation characteristics with the theory of isotachophoretic zone boundary, which is the only element of the isotachophoretic system with dispersional properties. This allows us to consider situations near the limits of the isotachophoretic method as far as both selectivity and sample amount (i.e., zone size) are concerned. Based on a simplified expression of the concentration profiles across the isotachophoretic zone boundary, separation and separation limits are described and discussed in terms of resolution, selectivity and zone capacity. Equations are derived showing the relationships between resolution, boundary width, selectivity, sample amount, and column and electrolyte conditions. A simple phenomenological equation is presented, expressing isotachophoretic resolution as a function of only sample amount (or sample zone length) and boundary width. A thermo-dynamic form of this equation is derived, which is shown to be similar to such an expression for resolution in zone electrophoresis. In both cases resolution is a function of sample selectivity, electric field strength and column length. A simple theoretical model for zone capacity is presented, making it possible to estimate the separation performance of isotachophoretic systems. Based on the presented theory, parallels between isotachophoresis and zone electrophoresis are discussed and both methods are compared.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601328
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