ISSN:
1573-5044
Keywords:
Conifer
;
transformation
;
virulence genes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract As a preliminary step in efforts to develop a successful protocol for Agrobacterium-mediated transformation of cotyledonary explants of Pinus pinea L. embryos, we tested the ability of embrionary exudates of this species to induce the expression of the virulence genes virA, virB, virC, virD, virE and virG in Agrobacterium tumefaciens containing vir: lacZ fusion constructs. The results obtained in the vir induction assay indicated the absence of bactericidal or bacteriostatic plant compounds affecting A. tumefaciens growth, and showed that cotyledonary and embrionary exudates of P. pinea are able to induce all virulence genes studied, except virG. The data suggest that A. tumefaciens can be used for gene transfer into this important forest and fruit species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006295622061
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