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  • medicinal plant  (3)
  • 1995-1999  (3)
  • 1
    Electronic Resource
    Electronic Resource
    in Vitro Somatic Embryogenesis in Typhonium Trilobatum Schott. (1999)
    Springer
    Plant growth regulation 27 (1999), S. 195-199 
    Details
    ISSN: 1573-5087
    Keywords: growth regulator ; in vitro ; medicinal plant ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An in vitro protocol was developed for the production of plants via somatic embryogenesis in callus cultures derived from petiole and leaf explants of Typhonium trilobatum. Optimum callus formation was achieved on semisolid Murashige and Skoog's [9] medium supplemented with 0.25 mg L−1 kinetin and 3.0 mgL−1 1-naphthaleneacetic acid (NAA) after 6 weeks of culture. Somatic embryogenesis was achieved upon transferring the callus to a medium containing 1.0 mg Lminus 1 kinetin and 0.25 mg Lminus 1 NAA. In vitro tuberization was also achieved on medium containing 1/2 strength MS basal salts supplemented with 1.0 mg L−1 Kinetin and 0.1 mg L−1 NAA. Embryo maturation and germination was achieved on the MS basal salts supplemented with 0.01 mg L−1 NAA and 2% (w/v) sucrose. Some thousands somatic embryo derived plantlets were hardened in the greenhouse and eventually planted in the open field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006278716645
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid clonal propagation of Plumbago zeylanica Linn. (1999)
    Springer
    Plant growth regulation 28 (1999), S. 1-4 
    Details
    ISSN: 1573-5087
    Keywords: growth regulator ; In vitro ; medicinal plant ; micropropagation ; Plumbago zeylanica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An efficient protocol was developed for in vitro clonal propagation of Plumbago zeylanica Linn. through nodal culture. Multiple shoots were induced from nodal explants of P. zeylanica on Murashige and Skoog's (1962) medium supplemented with 0.5 mg L−1 to 1.0 mg.L−1 6-benzyladenine and 3% (w/v) sucrose. Inclusion of IAA (0.01 mg L−1) in the culture medium improved the frequency of production of multiple shoots. Rooting was readily achieved upon transferring the shoots onto half-strength MS medium supplemented with 0.25 mg L−1 IBA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006146713143
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration from callus cultures of Psoralea corylifolia Linn (1997)
    Springer
    Plant growth regulation 22 (1997), S. 13-17 
    Details
    ISSN: 1573-5087
    Keywords: BA ; in vitro ; medicinal plant ; NAA ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plantlet regeneration via organogenesis was achieved in callus cultures derived form mature leaves, stems and leaves, petioles and roots of young seedling of Psoralea corylifolia on Murashige and Skoog medium supplemented with 2.5–3.0 mg L-1 BA, 1.0 mg L-1 NAA and 3% (w/v) sucrose. The rate of shoot bud regeneration was positively correlated with the concentration of hormones in the nutrient media. Shoot buds regenerated more readily from juvenile explants (seedling source) as compared to the mature explants. Addition of adenine sulphate (5 mg L-1) to the culture medium increased the growth of shoot buds. Optimum responses were obtained in hypocotyl and leaf explants using NAA in combination with BA, the highest rate of shoot bud regeneration being in hypocotyl explants. Rooting was readily achieved on the differentiated shoots on MS basal media without growth regulators. Regenerated plantlets were successfully established in the greenhouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005869404510
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