ISSN:
1573-4978
Keywords:
Escherichia coli
;
M1 RNA
;
Ribonuclease P
;
Ribozyme
;
RNA processing
;
tRNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A brief review of the genetic studies on ribonuclease P (RNase P) fromEscherichia coli is presented. Temperature-sensitive mutants ofE. coli defective in tRNA processing were isolated by screening cells which were unable to synthesize a suppressor tRNA at restrictive temperature. Structural analysis of accumulated tRNA precursors showed that the isolated mutants were defective in RNase P activity. Analyses of the mutants revealed that the enzyme is essential for the synthesis of all tRNA molecules in cells and that the enzymes consists of two subunits. Analyses of the isolated mutants revealed a possible domain structure of the RNA subunit of the enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00988714
Permalink
