ISSN:
1436-5073
Keywords:
enzyme mimetic
;
hydrogen peroxide determination
;
N,N-diethylaniline
;
spectrophotometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Various metalloporphyrins have been used as a catalyst instead of the peroxidase for the determination of hydrogen peroxide by formation of a dye from N,N-diethylaniline (DBA) and 4-aminoantipyrine. The difference of relative catalytic activity was investigated between enzyme and enzyme mimetics. FeT(4-TAP)P [5, 10, 15, 20-tetrakis (4-trimethyl-ammoniumphenyl)-21H, 23H-porphine] was shown as the best enzyme mimetic for horseradish peroxidase (HRP) among metalloporphyrins tested. 0 to 7.0 × 10−5 mol/L hydrogen peroxide was determined with good accuracy and reproducibility, and giving recovery of 99.7–100.7%. DEA was certified as a sensitive color reagent in enzyme mimetic assay of hydrogen peroxide, with the apparent molar absorptivity for hydrogen peroxide was 1.37 × 104 L/mol·cm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01242195
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