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  • 1
    Electronic Resource
    Electronic Resource
    Biological and nutritional aspects involved in fern multiplication (1999)
    Springer
    Plant cell, tissue and organ culture 56 (1999), S. 211-214 
    Details
    ISSN: 1573-5044
    Keywords: Asplenium nidus ; Dryopteris affinis sp. affinis ; fern ; Osmunda regalis ; propagation ; Pteris ensiformis ; tissue culture ; Woodwardia virginica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gametophytes of several species of ferns were mechanically triturated and the resulting homogenates cultured in vitro for propagation purposes. Differences in the time period from spore culture to sporophyte development were perceivable between species. For those species with a fast life cycle and high sporophyte production such as Woodwardia virginica and Dryopteris affinis sp. affinis, homogenization of gametophytes can be considered to be excellent method for propagation, yielding hundreds of sporophytes in a short period of time. Sporophyte formation was inhibited in O. regalis by the succesive application of homogenization to gametophytes regenerated by this technique. The effect of the culture medium composition on fern production was also studied in O. regalis and P. ensiformis gametophytes. In these species, sporophyte formation increased when the gametophytes were cultured in a medium containing water+0.7% agar. Addition of sucrose inhibited gametophyte development and induced their necrosis. The 1/2 dilution of Murashige and Skoog basal medium, without sucrose, favoured leaf expansion in P. ensiformis sporophytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006277229136
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  • 2
    Electronic Resource
    Electronic Resource
    Micropropagation and phase change in Blechnum spicant and Pteris ensiformis (1996)
    Springer
    Plant cell, tissue and organ culture 44 (1996), S. 261-265 
    Details
    ISSN: 1573-5044
    Keywords: apospory ; callus ; fern ; propagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizomes of juvenile sporophytes of Blechnum spicant L. and Pteris ensiformis L. cultured on MS medium with N6-benzyladenine alone (0.44–4.4 μM) or in combination with naphthaleneacetic acid (0.053–0.53 μM), respectively, gave rise to several proliferation centres located at the epidermal and inner parenchyma of this organ after one month in culture. Subculture of these rhizomes for one month in growth regulator-free medium allowed organization of internal proliferation centres, and regeneration of a large number of sporophytes. Subculture in the above-mentioned proliferation medium, induced phase change and development of aposporous gametophytes from surface-localized proliferation centres. Addition of naphthaleneacetic acid to the culture medium promoted proliferation of green globular bodies (GGB) in B. spicant and both rhizogenesis and callogenesis processes in P. ensiformis. GGB derived from rhizomes of B. spicant produced numerous sporophytes and from the point of view of micropropagation it is a good system to regenerate sporophytes in this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048534
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 93-97 
    Details
    ISSN: 1573-5044
    Keywords: callus, fern ; morphogenesis ; propagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The naturally-occurring apogamy of some ferns can be modified by culture conditions and growth regulators. Gametophytes of the apogamic fern Dryopteris affinis sp. affinis L., were cultured on Murashige and Skoog (MS) basal medium. Changes in concentration of MS medium components, sucrose, agar and different pH values were tested. The addition of benzyladenine (4.43 μM) and naphthalene acetic acid (0.53 μM) enhanced sporophyte proliferation on the gametophytes. After one month in culture, the gametophytes formed callus with a high morphogenic capacity. Culture of calli on medium without growth regulators yielded about 10,000 sporophytes per 1 g fresh weight of callus. This pattern of differentiation slowed with time to a point where only gametophyte regeneration was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043434
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro organogenesis of Polypodium cambricum (1999)
    Springer
    Plant cell, tissue and organ culture 57 (1999), S. 65-69 
    Details
    ISSN: 1573-5044
    Keywords: fern ; propagation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporophyte regeneration through green globular bodies (GGB) is affected by the supply of growth regulators as well as by the source of the donor explant. Rhizome, frond, petiole or root tip explants from juvenile sporophytes of Polypodium cambricum were cultured on media containing N6-benzyladenine (BA) or Kinetin alone or in combination with α-naphthaleneacetic acid (NAA). GGB production took place even in growth regulator-free medium. Addition of BA into the culture medium was very effective to induce formation of GGB in rhizome explants. Root tip explants exhibited the lowest organogenic capacity. Homogenization of BA-pretreated frond or rhizome was a powerful system to improve fern propagation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006348628114
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  • 5
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    Molecular cloning and disease association of hepatitis G virus: a transfusion-transmissible agent (1996)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1996-01-26
    Description: An RNA virus, designated hepatitis G virus (HGV), was identified from the plasma of a patient with chronic hepatitis. Extension from an immunoreactive complementary DNA clone yielded the entire genome (9392 nucleotides) encoding a polyprotein of 2873 amino acids. The virus is closely related to GB virus C (GBV-C) and distantly related to hepatitis C virus, GBV-A, and GBV-B. HGV was associated with acute and chronic hepatitis. Persistent viremia was detected for up to 9 years in patients with hepatitis. The virus is transfusion-transmissible. It has a global distribution and is present within the volunteer blood donor population in the United States.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Linnen, J -- Wages, J Jr -- Zhang-Keck, Z Y -- Fry, K E -- Krawczynski, K Z -- Alter, H -- Koonin, E -- Gallagher, M -- Alter, M -- Hadziyannis, S -- Karayiannis, P -- Fung, K -- Nakatsuji, Y -- Shih, J W -- Young, L -- Piatak, M Jr -- Hoover, C -- Fernandez, J -- Chen, S -- Zou, J C -- Morris, T -- Hyams, K C -- Ismay, S -- Lifson, J D -- Hess, G -- Foung, S K -- Thomas, H -- Bradley, D -- Margolis, H -- Kim, J P -- New York, N.Y. -- Science. 1996 Jan 26;271(5248):505-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Genelabs Technologies, Redwood City, CA 94063, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8560265" target="_blank"〉PubMed〈/a〉
    Keywords: Acute Disease ; Amino Acid Sequence ; Base Sequence ; Blood Donors ; Blood Transfusion/*adverse effects ; Blood-Borne Pathogens ; Chronic Disease ; Cloning, Molecular ; Consensus Sequence ; Disease Transmission, Infectious ; Flaviviridae/genetics ; Genome, Viral ; Hepatitis Viruses/chemistry/*genetics/isolation & purification ; Hepatitis, Viral, Human/epidemiology/transmission/*virology ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; RNA Viruses/chemistry/*genetics/isolation & purification ; RNA, Viral/blood/genetics ; Sequence Alignment ; United States/epidemiology ; Viral Proteins/chemistry/genetics ; Viremia/epidemiology/virology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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