ISSN:
1573-0603
Keywords:
Aphidicolin
;
Cell synchrony
;
CHO cells
;
Isoleucine-deficiency
;
S-phase block
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Procedures are described for synchronizing (1) rodent cells in G1 using the isoleucine-deprivation method, (2) normal diploid human and other kinds of fibroblasts in G0 using serum withdrawal, and (3) rodent and human cells in S phase using synchronized G0/G1 cells and aphidicolin. The effects of G0/G1-arrest and aphidicolin block on cyclins/cyclin dependent kinases and on the composition and structure of chromatin are summarized. Implications of these effects and the uses of the procedures are considered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00122162
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