Publication Date:
1999-12-03
Description:
Linker proteins function as molecular scaffolds to localize enzymes with substrates. In B cells, B cell linker protein (BLNK) links the B cell receptor (BCR)-activated Syk kinase to the phosphoinositide and mitogen-activated kinase pathways. To examine the in vivo role of BLNK, mice deficient in BLNK were generated. B cell development in BLNK-/- mice was blocked at the transition from B220+CD43+ progenitor B to B220+CD43- precursor B cells. Only a small percentage of immunoglobulin M++ (IgM++), but not mature IgMloIgDhi, B cells were detected in the periphery. Hence, BLNK is an essential component of BCR signaling pathways and is required to promote B cell development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pappu, R -- Cheng, A M -- Li, B -- Gong, Q -- Chiu, C -- Griffin, N -- White, M -- Sleckman, B P -- Chan, A C -- AI42787/AI/NIAID NIH HHS/ -- CA71516/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1999 Dec 3;286(5446):1949-54.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Immunology, Division of Rheumatology, Department of Medicine, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, MO 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10583957" target="_blank"〉PubMed〈/a〉
Keywords:
Adaptor Proteins, Signal Transducing
;
Aging
;
Animals
;
B-Lymphocyte Subsets/cytology/immunology
;
B-Lymphocytes/*cytology/immunology/*metabolism
;
Bone Marrow Cells/cytology/immunology
;
Carrier Proteins/genetics/*physiology
;
Cell Count
;
Cell Differentiation
;
Cell Separation
;
Cell Size
;
Flow Cytometry
;
Gene Targeting
;
Hematopoietic Stem Cells/*cytology/metabolism
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Immunoglobulin M/analysis
;
Leukopoiesis
;
Lymphoid Tissue/cytology/immunology
;
Mice
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Mice, Inbred C57BL
;
*Phosphoproteins
;
Receptors, Antigen, B-Cell/*metabolism
;
Second Messenger Systems
;
Signal Transduction
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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