ISSN:
1573-5036
Keywords:
FRE1
;
iron
;
polyadenylation
;
reductase
;
Strategy-I
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Fe(III) reduction at the root surface is an obligatory step in Fe uptake used by Strategy-I plants. The genes FRE1 and FRE2 are responsible for a similar mechanism of Fe acquisition in the cell membrane of Saccharomyces cerevisiae. We introduced the FRE1 gene into tobacco plants (Nicotiana tabacum L. cv. SR1). However, the transgenic tobacco showed no additional reductase activity, because the FRE1 transcripts from this transgenic tobacco were shorter than expected. Further investigation revealed that the coding region of the FRE1 gene was polyadenylated. We then reconstructed the whole sequence of the FRE1 gene and named it refre1 (reconstructed FRE1). The refre1 gene was introduced into tobacco plants. The transgenic plants carrying the refre1 gene produced full length mRNA and had constitutive ferric reductase activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1004591012061
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