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  • Evolutionary programming  (1)
  • Hemolysin  (1)
  • 1995-1999  (2)
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  • 1995-1999  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning, characterization, and sequencing of the hemolysin gene from Edwardsiella tarda (1996)
    Springer
    Archives of microbiology 165 (1996), S. 9-17 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsEdwardsiella tarda ; Hemolysin ; Hemolytic ; gene ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hemolysis is a major symptom of diseased eels infected by Edwardsiella tarda. The hemolysin gene of E. tarda strain ET16 was cloned into plasmid pSK and expressed in Escherichia coli. The mol. mass of the functional β-hemolysin was estimated to be approximately 34 kDa by gel filtration and by SDS-PAGE followed by in situ hemolysin activity analysis. The cloned fragment containing the β-hemolysin locus from E. tarda strain ET16 expressed in E. coli was estimated to be 5.3 kb in length; the deduced gene product was identical in mol. mass and properties to the extracellular products of E. tarda strain ET16. The presence of EcoRI and XbaI sites within the β-hemolysin gene of E. tarda was determined from the loss of hemolytic activity in subclones. Analysis of the DNA sequence of a 2,436-bp HaeIII-HindIII fragment that included EcoRI and XbaI sites revealed three ORFs organized as an operon that encoded three predicted polypeptides of 15,874, 7,055, and 34,804 Da. A 34-kDa polypeptide expressing hemolytic activity in cell lysates of the clone DH5α(pETH3E) is very likely the β-hemolysin encoded by the third ORF. The observation that hemolytic activity appeared in the culture medium of E. tarda, but not in that of E. coli strain DH5α(pETH3E) indicates the existence of a mechanism for transporting the hemolysin across the cell envelope in E. tarda that is different from that of E. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050290
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  • 2
    Electronic Resource
    Electronic Resource
    Optimized Overcomplete Signal Representation and its Applications to Time-frequency Analysis of Electrogastrogram (1998)
    Springer
    Annals of biomedical engineering 26 (1998), S. 859-869 
    Details
    ISSN: 1573-9686
    Keywords: Spectral analysis ; Matching pursuit ; Stomach ; Gastric motility ; Electrogastrography ; Evolutionary programming ; Signal analysis ; Electrogastrogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The electrogastrogram (EGG) is a surface measurement of gastric myoelectrical activity. The normal frequency of gastric myoelectrical activity in humans is 3 cycles/min. Abnormal frequencies in gastric myoelectrical activity have been found to be associated with functional disorders of the stomach. The aim of this article was, therefore, to develop new time-frequency analysis methods for the detection of gastric dysrhythmia from the EGG. A concept of overcomplete signal representation was used. Two algorithms were proposed for the optimization of the overcomplete signal representation. One was a fast algorithm of matching pursuit and the other was based on an evolutionary program. Computer simulations were performed to compare the performance of the proposed methods in comparison with existing time-frequency analysis methods. It was found that the proposed algorithms provide higher frequency resolution than the short time Fourier transform and Wigner-Ville distribution methods. The practical application of the developed methods to the EGG is also presented. It was concluded that these methods are well suited for the time-frequency analysis of the EGG and may also be applicable to the time-frequency analysis of other biomedical signals. © 1998 Biomedical Engineering Society. PAC98: 8780+s, 0705Kf
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1114/1.69
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