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  • 1
    Electronic Resource
    Electronic Resource
    Does root glutamine synthetase control plant biomass production in Lotus japonicus L.? (1999)
    Springer
    Planta 209 (1999), S. 495-502 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Biomass production ; Glutamine synthetase ; Lotus (N metabolism) ; Nitrate uptake ; Recombinant inbred lines ; Transgenic plant (Lotus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. To investigate the contribution of root cytosolic glutamine synthetase (GS) activity in plant biomass production, two different approaches were conducted using the model legume Lotus japonicus. In the first series of experiments, it was found that overexpressing GS activity in roots of transgenic plants leads to a decrease in plant biomass production. Using 15N labelling it was shown that this decrease is likely to be due to a lower nitrate uptake accompanied by a redistribution to the shoots of the newly absorbed nitrogen which cannot be reduced due to the lack of nitrate reductase activity in this organ. In the second series of experiments, the relationship between plant growth and root GS activity was analysed using a series of recombinant inbred lines issued from the crossing of two different Lotus ecotypes, Gifu and Funakura. It was confirmed that a negative relationship exists between root GS expression and plant biomass production in both the two parental lines and their progeny. Statistical analysis allowed it to be estimated that at least 13% of plant growth variation can be accounted for by variation in GS activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050753
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  • 2
    Electronic Resource
    Electronic Resource
    Advances in the positional cloning of nodulation genes in soybean (1996)
    Springer
    Plant and soil 186 (1996), S. 1-7 
    Details
    ISSN: 1573-5036
    Keywords: arbitrary primers ; BAC ; BSA ; DAF ; genetic markers ; nodulation ; positional cloning ; symbiosis genes ; soybean ; YAC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The positional cloning of genes involved in plant control of infection and nodule formation in legumes requires the development of improved tools for the analysis of large genomes and cloning of high molecular weight DNA. We have used bulked segregant analysis (BSA) and DNA amplification fingerprinting (DAF) with arbitrary oligonucleotide primers to detect polymorphisms linked to genes important to nodulation in soybean (Glycine max L. Merrill). Three loci controlling legume nodulation (nts-1, rj1 and rj6) and one involved in early nodule development (enod2) were studied. Wild-type Bragg and EMS-induced mutants defective in autoregulation (nts382) or nodulation (nod49 and nod139) were crossed with G. soja (Sieb. and Zucc.), for the analysis of segregants in F2 and F3 populations. DNA pools from wild-type and mutant individuals, or DNA pools based on RFLP pattern, were screened with sets of structured (mini-hairpin) and unstructured primers, identifying polymorphic DNA. DAF with mini-hairpin primers, template endonuclease cleaved DAF (tecMAAP), and arbitrary signatures from amplification profiles (ASAP) were especially successful in detecting linked polymorphisms. Putative markers were confirmed by individual analysis of segregants, and some of them converted into sequence-characterized amplified regions (SCAR). These markers will be used for high density mapping of the relevant genomic regions and as landmarks for linking cloned soybean DNA from yeast and bacterial artificial chromosome (YAC and BAC) libraries. At present, the partial YAC library (avg. insert size = 200 kb) represents about 20% of the soybean genome. YAC endclones were isolated using vectorette PCR, and consisted of unique or repeated DNA. Together with YAC-specific signatures generated with mini-hairpin primers, they will be used in the construction of contigs for positional cloning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035049
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