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  • Brassica napus  (3)
  • timber pest  (2)
  • 1995-1999  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Deathwatch beetle, Xestobium rufovillosum, in historical buildings: monitoring the pest and its predators (1999)
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 97-104 
    Details
    ISSN: 1570-7458
    Keywords: deathwatch beetle ; Xestobium rufovillosum ; Coleoptera ; Anobiidae ; timber pest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Trapping and monitoring experiments were conducted in the roof spaces of four buildings infested with deathwatch beetle, Xestobium rufovillosum de Geer (Coleoptera: Anobiidae). Data from sticky traps and an ultra-violet insectocutor showed that adult deathwatch beetles were trapped from May to July. The beetles were attracted to natural and UV light, and more beetles were caught on white coloured traps than yellow, blue or red traps. Deathwatch beetles comprised 30–40% of all arthropods caught. The weekly trap catch of all arthropods, including deathwatch beetle, was positively correlated with ambient temperature. Adult beetles flew in buildings at ambient temperatures greater than 17 °C. Arthropods caught in the buildings were categorised as resident, over-wintering or non-resident arthropods. Predatory spiders comprised 13% of arthropods caught and the predatory beetle, Korynetes caeruleus de Geer, was found in all four buildings. There was no evidence of other predators or parasitoids of the deathwatch beetle
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003824910137
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  • 2
    Electronic Resource
    Electronic Resource
    Host selection behaviour of deathwatch beetle, Xestobium rufovillosum: Oviposition preference choice assays testing old vs new oak timber, Quercus sp. (1998)
    Springer
    Entomologia experimentalis et applicata 89 (1998), S. 193-199 
    Details
    ISSN: 1570-7458
    Keywords: deathwatch beetle ; Xestobium rufovillosum ; Coleoptera ; Anobiidae ; Quercus sp. ; host selection behaviour ; oviposition ; timber pest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a choice bio-assay adult female deathwatch beetles were offered two dendrochronologically dated wood blocks from oak timber to study oviposition preference behaviour. There was a clear preference for ovipositing on old wood dating from the 13th to 19th centuries rather than new wood from the 20th century. Control, same-century choice, experiments showed that beetles will oviposit on young wood and that the age of the wood does not alter the overall oviposition potential. Oviposition frequency varied with insect age. Fecundity of insects collected from an infested building was similar to that of insects maintained in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003558723537
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  • 3
    Electronic Resource
    Electronic Resource
    High frequency production of doubled haploid plants of Brassica napus cv. Topas derived from colchicine-induced microspore embryogenesis without heat shock (1996)
    Springer
    Plant cell reports 15 (1996), S. 668-671 
    Details
    ISSN: 1432-203X
    Keywords: Brassica napus ; Chromosome doubling ; Colchicine ; Doubled haploids ; Microspore embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This report describes a very high genome doubling efficiency of Brassica napus cv. Topas plants, derived from microspores induced to undergo embryogenesis with a colchicine treatment, without the use of a heat treatment. The plants showed normal growth and development, and 90% were fertile. In contrast, only 6% of the plants derived from heat-induced embryos were fertile diploids. All cytological analysis of the progeny of fertile plants showed 2n=38 chromosomes. These results show that colchicine can simultaneously induce microspore embryogenesis and double the ploidy level to produce doubled haploid plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231921
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  • 4
    Electronic Resource
    Electronic Resource
    Induction of embryogenesis with colchicine instead of heat in microspores ofBrassica napus L. cv. Topas (1996)
    Springer
    Planta 198 (1996), S. 433-439 
    Details
    ISSN: 1432-2048
    Keywords: Brassica napus ; Colchicine ; Heat shock ; Microspore embryogenesis ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis fromBrassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction inB. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00620060
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  • 5
    Electronic Resource
    Electronic Resource
    Cellular changes during heat shock induction and embryo development of cultured microspores ofBrassica napus cv. Topas (1995)
    Springer
    Protoplasma 185 (1995), S. 106-112 
    Details
    ISSN: 1615-6102
    Keywords: Brassica napus ; Embryogenesis ; Heat shock ; Induction ; Microspore embryogenesis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Brassica napus cv. Topas microspores, isolated and cultured near the time of the first pollen mitosis and subjected to a heat treatment of 24 h, can be induced to develop into haploid embryos. This is a study of microspore structure during induction and embryo determination. Early during the 32.5 °C incubation period the nucleus moved away from the edge of the cell, and granules, 30 to 60 nm in diameter, appeared in the mitochondria and as a cluster in the cytoplasm. Cells divided symmetrically and at the end of the heat treatment, acquired the features of induced bicellular structures described previously. The features persisted as the cells divided randomly within the exine for 4–7 days following heat induction. Multicellular structures released from the exine underwent periclinal divisions resulting in protoderm differentiation of the globular embryo, thus determining embryo development. The cytoplasm of early heart-stage embryos contains abundant polyribosomes. Non-embryogenic development was indicated by large accumulations of starch and/or lipid and thickened cell walls or an unorganized pattern of cell division following release of the multicellular structures from the exine. Embryogenesis is discussed in terms of induction, embryo determination and development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01272758
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