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  • Branchiopoda  (1)
  • Cry1Ea4  (1)
  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 437-441 
    ISSN: 1573-0972
    Keywords: Bacillus thuringiensis ; Cry1Ea4 ; novel protein ; sequencing ; toxicity comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A new cry gene (cry1Ea4) was cloned and sequenced from a Bacillus thuringiensis isolate native to Mexico (LBIT-147). The gene coded for a 133kDa protoxin which had greater than 99% homology with the holotype Cry1Ea1, as only four mismatches were found between the two amino acid sequences. When the Cry1Ea4 toxin was expressed in a crystal-negative strain of B. thuringiensis, bipyramidal crystals were produced. Purified crystals from this recombinant strain and from the holotype (Cry1Ea1) were bioassayed against first instar larvae of the tobacco hornworm. Statistically different mean LC50 values indicated that Cry1Ea4 was more toxic than its holotype. This increase in toxicity may be attributed to the three amino acids which differ from the holotype sequence in the toxic fragment.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: Crustacea ; Branchiopoda ; community ecology ; sympatry ; co-occurrence ; temporary pools
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We present observations on the frequency of large branchiopod associations found in north-central Mexico, and in Arizona, USA. Of a total of 25 species involved in these assemblages, 12 were common in both areas. Fifty-eight (43.3%) of the ponds in Mexico, and seventy-eight (47%) of those in Arizona had two or more species present. The combinations of species which occurred with highest frequency were Streptocephalus mackini with Thamnocephalus platyurus for Arizona, and T. platyurus, Triops sp., and Leptestheria compleximanus for Mexico. In Mexico, and Arizona, as in many parts of the world, multispecies assemblages of large branchiopods are a common phenomenon. Therefore, the ’common rule‘ of’one-phyllopod-per-habitat‘ advanced by Weise (1964) is inconsistent with field observations. Furthermore, since cases of co-occurrence of two or more congeneric species of Anostraca and Notostraca are not uncommon, generalizations about congeners not coexisting remain applicable only for the Spinicaudata and Laevicaudata. On the basis of the literature, and of observations in the field and in the laboratory, we list potential factors contributing to the co-occurrence of several species within a pond.
    Type of Medium: Electronic Resource
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