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  • Bipolaris oryzae  (2)
  • Bjerkandera adusta  (2)
  • 1995-1999  (4)
  • 1
    ISSN: 1611-4663
    Keywords: Lignin biodegradation ; Manganese peroxidase ; Bjerkandera adusta ; Acetone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The reaction of manganese peroxidase (MnP) of the white-rot fungusBjerkandera adusta with synthetic lignin dehydrogenation polymer, DHP) in acetone medium was investigated. Gel-permeation chromatography of the DHP treated by MnP demonstrated depolymerization of syringyl DHP in the reaction mixture containing 70% acetone; moreover, concomitant repolymerization occurred to give highly polymerized products. Guaiacyl DHP was only repolymerized by MnP in the same acetone solution without giving degradation products. Addition of ascorbic acid to reaction mixtures containing acetone resulted in preferential depolymerization of syringyl DHP.
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  • 2
    ISSN: 1618-2545
    Keywords: Bjerkandera adusta ; lignin peroxidase ; manganese peroxidase ; rotary-solid type fermenter ; solid culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Manganese peroxidase (MnP) and lignin peroxidase (LiP) were produced by growing a white-rot fungusBjerkandera adusta statically, on a wood meal/wheat bran culture in flasks. MnP and LiP reached their maximum activity after 6 and 19 days of inoculation, respectively. Both MnP and LiP are thought to be important enzymes in lignin biodegradation byB. adusta. Ion exchange chromatography showed thatB. adusta produced a single LiP and a single MnP enzyme in wood meal/wheat bran culture. These enzymes were separated and characterized. The molecular weight of MnP was 46,500 with a pl of 3.9. The molecular weight of LiP was estimated to be 47,000 with a pl of 3.5. Spectral analysis demonstrated that both enzymes are heme proteins. Production of these enzymes was also achieved using a rotarysolid culture fermenter. MnP, LiP and veratryl alcohol oxidase were produced byB. adusta in the fermenter.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Mycoscience 40 (1999), S. 363-366 
    ISSN: 1618-2545
    Keywords: Bipolaris oryzae ; blue light ; reactivation ; sporulation ; UV radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporulation inBipolaris oryzae was induced by UV radiation (295 nm), but the number of conidia gradually decreased with increasing duration of UV radiation longer than 1 min. The inductive effect of UV radiation can be nullified by blue light (459 nm) applied immediately before or after inductive UV radiation shorter than 1 min. In contrast, the number of conidia increased with an increasing duration of blue light applied after inductive UV radiation longer than 1 min, but not if it was applied before UV radiation. The present study firstly revealed the possibility of photoreactivation inB. oryzae sporulation.
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  • 4
    ISSN: 1618-2545
    Keywords: Bipolaris oryzae ; Colletotrichum lagenarium ; germ tube growth ; infection structure ; phototropism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The germ tubes ofColletotrichum lagenarium showed negative phototropism to UV-blue (300–520 nm) and far-red (〉700 nm) regions with maximum in the near ultraviolet (NUV) region, while monochromatic radiations of 575–700 nm (yellow-red region) induced positive phototropism with maximum in the red region. Green light (520–575 nm) was ineffective. Negative phototropism-inducing wavelength regions inhibited germ tube growth and positive phototropism-inducing wavelength regions promoted it significantly.Bipolaris oryzae did not show any phototropic response and light did not affect the germ tube growth. These results indicate that the lens effect, in combination with the light growth reaction and light growth inhibition, is the mechanism of the phototropism of germ tubes ofC. lagenarium. NUV radiation, which induced negative phototropism ofC. lagenarium, promoted appressorium formation, while red light, which induced positive phototropism, suppressed it significantly. In the case ofB. oryzae, light did not affect the infection structure formation. These results indicate that negative phototropism of germ tubes ofC. lagenarium favors the infection process by facilitating the contact of the tips of germ tubes with the host surface, while positive phototropism has the opposite effect.
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