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  • Biochemistry and Biotechnology  (1)
  • Glycine max (L.) Merr  (1)
  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Alteration of the omega-3 fatty acid desaturase gene is associated with reduced linolenic acid in the A5 soybean genotype (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 356-359 
    Details
    ISSN: 1432-2242
    Keywords: Key words Soybean ; Glycine max (L.) Merr ; Linolenic acid ; Omega-3 fatty acid desaturase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Reducing the linolenic acid (18 : 3ω  3,6,9) concentration of soybean [Glycine max (L.) Merr.] oil may lessen the need for chemical hydrogenation and enhance flavor stability. Soybean genotypes A5 and A23 have reduced linolenic acid concentration compared with current cultivars. Seed linolenic acid is synthesized primarily by the ω-3 fatty acid desaturase located in the microsomes. The objective of this research was to study whether this enzyme has a role in reducing the fatty acid levels in the soybean genotypes A5 and A23. DNA from A5 and A23 was analyzed by gel-blot hybridization with a cDNA encoding the ω-3 fatty acid desaturase. A5 and lines selected from it have a DNA fragment missing compared to A23 and lines with normal linolenic acid concentration. Seventy F4:5 lines from a population segregating for linolenic acid concentration were scored for presence or absence of the fragment. The absence of the fragment was significantly (P?0.0001) associated with a reduced linolenic acid level and accounted for 67% of the variation for linolenic acid in the population. These results suggest that the reduced linolenic acid concentration in A5 was at least partially the result of a full or partial deletion of a microsomal ω-3 desaturase gene. No DNA polymorphisms were found for the desaturase gene in A23, so no mutations could be studied in this line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050423
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  • 2
    Electronic Resource
    Electronic Resource
    Denitrification and nitric oxide reduction in an aerobic toluene-treating biofilter (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 408-415 
    Details
    ISSN: 0006-3592
    Keywords: nitric oxide ; NOx ; flue gas ; denitrification ; aerobic ; biofilter ; aerosol ; biomass control ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The presence of significant denitrification activity in an aerobic toluene-treating biofilter was demonstrated under batch and flow-through conditions. N2O concentrations of 9.2 ppmv were produced by denitrifying bacteria in the presence of 15% acetylene, in a flow-through system with a bulk gas phase O2 concentration of 〉17%. The carbon source for denitrification was not toluene but a byproduct or metabolite of toluene catabolism. Denitrification conditions were successfully used for the reduction of 60 ppmv nitric oxide to 15 ppmv at a flow rate of 3 L min-1 (EBRT of 3 min) in a fully aerated, 17% v/v O2 (superficially aerobic) biofilter. Higher NO removal efficiency (97%) was obtained by increasing the toluene supply to the biofilter. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:408-415, 1998.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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