ISSN:
1432-0983
Keywords:
Cellulase
;
Aspergillus kawachii
;
cDNA cloning
;
Expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The cDNA encoding the endo-β-1,4-glucanase (carboxymethylcellulase; CMCase-I) from Aspergillus kawachii IFO 4308 was cloned. Nucleotide-sequence analysis of the cloned cDNA insert showed a 717-bp open reading frame that encoded a protein of 239 amino-acid residues. The predicted amino-acid sequence of the mature protein had considerable homology with the protein sequence of the FI-CMCase of Aspergillus aculeatus. The cDNA was introduced into Saccharomyces cerevisiae. The expressed enzyme had carboxylmethylcellulase acitivity, identified by clear zones on a CMC-agar plate after Congo Red staining.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00311212
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