ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Chemistry  (22)
  • Life and Medical Sciences  (9)
  • Analytical Chemistry and Spectroscopy
  • 1995-1999  (31)
  • 1
    Electronic Resource
    Electronic Resource
    Neutron Diffraction Study of [K(18-crown-6)] [(PPh3)2ReH6Cr(CO)3], a Bimetallic Donor-Acceptor Complex⋆ (1998)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 1998 (1998), S. 851-854 
    Details
    ISSN: 1434-1948
    Keywords: Neutron diffraction ; Donor-acceptor complex ; Bridging hydride ligand ; Rhenium ; Chromium ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A neutron diffraction analysis was carried out at 120 K on a single crystal of [K(18-crown-6)][(PPh3)2ReH6Cr(CO)3] · THF in order to locate the hydride ligands. Three of the hydrides are bound terminally to the Re atom while the other three bridge the Re-Cr bond; the bridging hydrides are closer to the Re atom. This compound can be thought of as a donor-acceptor complex, with [(PPh3)2ReH6]- acting as the donor to the Cr(CO)3 fragment. Average distances and angles: Re-Cr = 2.58(1), Re-H(br) = 1.75(1), Cr-H(br) = 1.92(2), Re-H(t) = 1.69(1) Å; Re-H-Cr = 89.1(7)°. Final agreement factors: R(F) = 6.4% for 3443 reflections with I 〉 2σ(I), and R(F) = 8.3% for all data (4195 reflections).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Selective peptidic and peptidomimetic inhibitors of Candida albicans myristoylCoA: Protein N-myristoyltransferase: A new approach to antifungal therapy (1997)
    New York : Wiley-Blackwell
    Biopolymers 43 (1997), S. 43-71 
    Details
    ISSN: 0006-3525
    Keywords: antifungal therapy ; Candida albicans ; peptidic inhibitors ; peptidomimetic inhibitors ; myristoylCoA ; protein N-myristoyltransferase ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: MyristoylCoA: protein N-myristoyltransferase (NMT) catalyzes the cotranslational covalent attachment of a rare cellular fatty acid, myristate, to the N-terminal Gly residue of a variety of eukaryotic proteins. The myristoyl moiety is often essential for expression of the biological functions for these proteins. Attachment of C14:0 alone provides barely enough hydrophobicity to allow stable association with membranes. The partitioning of N-myrisotyl-proteins is therefore often modulated by “switches” that function through additional covalent or noncovalent modifications.Candida albicans, the principal cause of systemic fungal infection in immunocompromised humans, contains a single NMT gene that is essential for its viability. The functional properties of the acylCoA binding site of human and C. albicans NMT are very similar. However, there are distinct differences in their peptide binding sites. An ADP ribosylation factor (Arf) is included among the few cellular protein substrates of the fungal enzyme. Alanine scanning mutagenesis of an octapeptide derived from an N-terminal Arf sequence (GLYASKLS-NH2) disclosed that Gly1, Ser5, and Lys6 play predominant roles in binding. ALYASKLS-NH2 is an inhibitor competitive for peptide [Ki(app) = 15.3±6.4 μM] and noncompetitive for myristoylCoA. Remarkably, replacement of the N-terminal tetrapeptide with an 11-aminoundecanoyl group results in a competitive inhibitor (11-aminoundecanoyl-SKLS-NH2) that is ∼ 40-fold more potent [Ki(app) = 0.40 ± 0.03 μM] than the starting octapeptide. Removal of Leu-Ser from the C-terminus generates a competitive dipeptide inhibitor (11-aminoundecanoyl-SK-NH2) with a Ki(app) of 11.7 ± 0.4 μM, equivalent to that of the starting octapeptide. A derivative dipeptide inhibitor containing a C-terminal N-cyclohexylethyl lysinamide moiety has the advantage of being more potent (IC50 = 0.11 ± 0.03 μM) and resistant to digestion by cellular carboxypeptidases. Rigidifying the flexible aminoundecanoyl chain results in very potent general NMT inhibitors (IC50 = 40-50 nM). Substituting a 2-methylimidazole for the N-terminal amine and adding a benzylic α-methyl group with R streochemistry to the rigidifying element produces even more potent inhibitors (IC50 = 20-50 nM) that are up to 500-fold selective for the fungal compared to human enzyme. A related less potent member of this series of compounds in fungistatic. Its growth inhibitory effects are associated with a reduction in cellular protein N-myristoylation, judged using cellular Arf as a reporter. These studies establish that NMT is a new antifungal target. © 1997 John Wiley & Sons, Inc. Biopoly 43: 43-71, 1997
    Additional Material: 75 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 3
    Electronic Resource
    Electronic Resource
    Heparin-binding EGF-like growth factor in the human prostate: Synthesis predominantly by interstitial and vascular smooth muscle cells and action as a carcinoma cell mitogen (1998)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 68 (1998), S. 328-338 
    Details
    ISSN: 0730-2312
    Keywords: cell proliferation ; tumor progression ; EGF receptor ; ErbB ; HER1 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is an activating ligand for the EGF receptor (HER1/ErbB1) and the high-affinity receptor for diphtheria toxin (DT) in its transmembrane form (proHB-EGF). HB-EGF was immunolocalized within human benign and malignant prostatic tissues, using monospecific antibodies directed against the mature protein and against the cytoplasmic domain of proHB-EGF. Prostate carcinoma cells, normal glandular epithelial cells, undifferentiated fibroblasts, and inflammatory cells were not decorated by the anti-HB-EGF antibodies; however, interstitial and vascular smooth muscle cells were highly reactive, indicating that the smooth muscle compartments are the major sites of synthesis and localization of HB-EGF within the prostate. In marked contrast to prostatic epithelium, proHB-EGF was immunolocalized to seminal vesicle epithelium, indicating differential regulation of HB-EGF synthesis within various epithelia of the reproductive tract. HB-EGF was not overexpressed in this series of cancer tissues, in comparison to the benign tissues. In experiments with LNCaP human prostate carcinoma cells, HB-EGF was similar in potency to epidermal growth factor (EGF) in stimulating cell growth. Exogenous HB-EGF and EGF each activated HER1 and HER3 receptor tyrosine kinases and induced tyrosine phosphorylation of cellular proteins to a similar extent. LNCaP cells expressed detectable but low levels of HB-EGF mRNA; however, proHB-EGF was detected at the cell surface indirectly by demonstration of specific sensitivity to DT. HB-EGF is the first HER1 ligand to be identified predominantly as a smooth muscle cell product in the human prostate. Further, the observation that HB-EGF is similar to EGF in mitogenic potency for human prostate carcinoma cells suggests that it may be one of the hypothesized stromal mediators of prostate cancer growth. J. Cell. Biochem. 68:328-338, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 4
    Electronic Resource
    Electronic Resource
    Hydrocarbon/hydrogen mixed gas permeation in poly(1-trimethylsilyl-1-propyne) (PTMSP), poly(1-phenyl-1-propyne) (PPP), and PTMSP/PPP blends (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 34 (1996), S. 2613-2621 
    Details
    ISSN: 0887-6266
    Keywords: poly(1-trimethylsilyl-1-propyne) ; poly(1-phenyl-1-propyne) ; blends ; hydrocarbons ; hydrogen ; mixed gas transport ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The gas permeation properties of poly(1-trimethylsilyl-1-propyne) (PTMSP), poly(1-phenyl-1-propyne) (PPP), and blends of PTMSP and PPP have been determined with hydrocarbon/hydrogen mixtures. For a glassy polymer, PTMSP has unusual gas permeation properties which result from its very high free volume. Transport in PPP is similar to that observed in conventional, low-free-volume glassy polymers. In experiments with n-butane/hydrogen gas mixtures, PTMSP and PTMSP/PPP blend membranes were more permeable to n-butane than to hydrogen. PPP, on the other hand, was more permeable to hydrogen than to n-butane. As the PTMSP composition in the blend increased from 0 to 100%, n-butane permeability increased by a factor of 2600, and n-butane/hydrogen selectivity increased from 0.4 to 24. Thus, both hydrocarbon permeability and hydrocarbon/hydrogen selectivity increase with the PTMSP content in the blend. The selectivities measured with gas mixtures were markedly higher than selectivities calculated from the corresponding ratio of pure gas permeabilities. The difference between mixed gas and pure gas selectivity becomes more pronounced as the PTMSP content in the blend increases. The mixed gas selectivities are higher than pure gas selectivities because the hydrogen permeability in the mixture is much lower than the pure hydrogen permeability. For example, the hydrogen permeability in PTMSP decreased by a factor of 20 as the relative propane pressure (p/psat) in propane/hydrogen mixtures increased from 0 to 0.8. This marked reduction in permanent gas permeability in the presence of a more condensable hydrocarbon component is reminiscent of blocking of permanent gas transport in microporous materials by preferential sorption of the condensable component in the pores. The permeability of PTMSP to a five-component hydrocarbon/hydrogen mixture, similar to that found in refinery waste gas, was determined and compared with published permeation results for a 6-Å microporous carbon membrane. PTMSP exhibited lower selectivities than those of the carbon membrane, but permeability coefficients in PTMSP were nearly three orders of magnitude higher. © 1996 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 5
    Electronic Resource
    Electronic Resource
    A new continuous biofilm bioreactor for immobilized oil-degrading filamentous fungi (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 49 (1996), S. 20-25 
    Details
    ISSN: 0006-3592
    Keywords: filamentous fungi ; immobilization ; biofilm bioreactor ; oil emulsion ; degradation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new type of horizontal biofilm bioreactor for continuous bioconversion of emulsified oily substrate by immobilized growing biofilm of filamentous fungi was designed, constructed, and feasibility tested. The new reactor design provides “self”-immobilization of homogenized mycelium leading to even biofilm development. This was accomplished by using stainless steel screens of optimal mesh, mounted in parallel and stretching outward from a main rotating axis of a biological rotating contractor. Each screen was equipped with a pair of stainless steel blades mounted on supports allowing for continuous biofilm “shaving” beyond a predetermined thickness, thus retaining freshly growing active biofilm surface. The feasibility of the new bioreactor was demonstrated by decalactone production from emulsified castor oil by immobilized filamentous fungi (Tyromyces sambuceus). The combination of oriented metal screens and moving blades was found to be highly effective for a model system in maintaining stable substrate emulsion in the reactor in either batchwise or continuous processing, as well as maintaining biofilm thickness with continuous removal of excess growing hyphae. © 1996 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 6
    Electronic Resource
    Electronic Resource
    Risk based package design (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 16 (1997), S. 14-17 
    Details
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The shipment of hazardous chemicals can pose signficant risk to the general public and the environment. These shipments are made in a variety of packages ranging from small bottles to large tank trucks, tank cars, and barges. However, the many standards and regulations that have been established to govern the design and use of these packages define what many consider to be the minimum requirements for risk management. This paper presents a methodology that can be used to more thoroughly identify the risk minimization options and verify the design of a package for a particular service. This method is based on the concept of a threat analysis of the proposed movement of the hazardous chemical. The threat analysis looks for unusual (but realistic) threats to the package that may result in the release of the hazardous chemical to the environment. Such unusual threats may include events such as: Dropping of the package during loading; Accident enroute; External fire during shipment; Random acts of vandalism (using the package as a practice target); Puncture (fork lift collision with package, rail/truck accident); Crushing (sudden starting or stopping). By conducting an engineering analysis of the strength and ability of the container to withstand these unusual events, a package design that can withstand the threats indentified in the threat analysis can be defined.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prs.680160107
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Fixation and stabilization of Escherichia coli cells displaying genetically engineered cell surface proteins (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 52 (1996), S. 625-630 
    Details
    ISSN: 0006-3592
    Keywords: stabilization ; β-lactamase ; bacterial cell surface engineering ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A large biotechnological potential is inherent in the display of proteins (e.g., enzymes, single-chain antibodies, on the surface of bacterial cells) (Georgiou et al., 1993). Applications such as immobilized whole-cell biocatalysts or cellular adsorbents require cell fixation to prevent disintegration, stabilization of the anchored protein from leakage, denaturation or proteolysis, and total loss of cell viability, preventing medium and potential product contamination with cells. In this article we describe the adaptation of a simple two-stage chemical crosslinking procedure based on “bi-layer encagement” (Tor et al., 1989) for stabilizing Escherichia coli cells expressing an Lpp-OmpA (46-159)-β-lactamase fusion that displays β-lactamase on the cell surface. Bilayer crosslinking and coating the bacteria with a polymeric matrix is accomplished by treating the cells first with either glutaraldehyde or polyglutaraldehyde, followed by secondary crosslinking with polyacrylamide hydrazide. These treatments resulted in a 5- to 25-fold reduction of the thermal inactivation rate constant at 55°C of surface anchored β-lactamase and completely prevented the deterioration of the cells for at least a week of storage at 4°C. The stabilization procedure developed paves the way to scalable biotechnological applications of E. coli displaying surface anchored proteins as whole-cell biocatalysts and adsorbents. © 1996 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 8
    Electronic Resource
    Electronic Resource
    Regulation of P120 mRNA levels during lymphocyte stimulation: Evidence that the P120 gene shares properties with early and late genes (1996)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 60 (1996), S. 458-468 
    Details
    ISSN: 0730-2312
    Keywords: nucleolar protein ; rRNA ; G1-phase ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: P120 is a growth-regulated nucleolar protein, the expression of which is required for G1- to S-phase transition in lymphocytes. P120 appears to be involved in ribosomal biogenesis presumptively through its putative role as a rRNA methyltransferase. To better understand the role of P120 in cell cycle progression, we examined the regulation of the P120 gene in resting lymphocytes and in mitogen-stimulated lymphocytes as they progress from G1-phase toward S-phase. P120 mRNA was detected after the immediate early gene c-fos and persisted as the cells approached S-phase. A decrease in P120 mRNA coincided with the expression of histone H3 mRNA. The level of P120 mRNA increased as cells proceeded through G1-phase, and this increase was attributed to a more than threefold increase in the P120 transcription rate and an increase in P120 mRNA stability. The P120 gene is transcribed in resting lymphocytes, although the steady-state level of P120 is small or nonexistent. P120 mRNA accumulates in resting cells in the presence of the protein synthesis inhibitor cycloheximide. Furthermore, the steady-state level of P120 mRNA increases in the presence of cycloheximide after PHA-stimulation; this level does not increase in cells not treated with this protein synthesis inhibitor. The presence of cycloheximide increases both the transcription rate of the P120 gene and the stability of P120 mRNA. These studies indicate that P120 expression is cell cycle regulated in a complex manner and that the P120 gene has properties of both early and late genes. This time ordered regulation for P120 expression may represent a necessary step for the cell cycle associated increase in ribosomal biogenesis that is required for G1- to S-phase transition. © 1996 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 9
    Electronic Resource
    Electronic Resource
    Synthesis and thermal transitions of a soluble, main chain, nematic liquid crystalline polymer exhibiting a kinetically trapped, disordered structure (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 34 (1996), S. 1347-1361 
    Details
    ISSN: 0887-6266
    Keywords: liquid crystalline polymer ; nematic ; isotropic/nematic transition ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: An aromatic copolyester composed of 25 mol % phenyl hydroquinone, 10 mol % isophthalic acid, 40 mol % chloroterephthalic acid, and 25 mol % t-butyl hydroquinone (PICT) has been synthesized. This amorphous, glassy polymer is soluble in common organic solvents such as methylene chloride. Thin, solution-cast films may be prepared which are in a metastable, vitrified, optically isotropic state. On first heating of an isotropic film at 20°C/min in a calorimeter, one glass transition is observed at low temperature (approximately 49°C) and is ascribed to the glass/rubber transition of the metastable, isotropic polymer. This thermal event is followed by a small exotherm due to the development of order during the scan, which results in a second Tg at approximately 125°C. This Tg is associated with the glass/rubber transition of the ordered polymer. Nematic order can be developed by thermal annealing. The lower Tg increases toward the upper Tg as annealing time is increased. For an initially isotropic film annealed at 90°C, the increase of the lower Tg with annealing time and the increase in birefringence observed by optical microscopy are governed by similar kinetics. Isotropization occurs in the temperature range of 250-300°C. The nematic polymer is slightly more dense than its isotropic analog. No detectable differences between isotropic and nematic samples were observed in rotating frame proton spin lattice relaxation times. © 1996 John Wiley & Sons, Inc.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
  • 10
    Electronic Resource
    Electronic Resource
    Pure hydrocarbon sorption properties of poly(1-trimethylsilyl-1-propyne) (PTMSP), poly(1-phenyl-1-propyne) (PPP), and PTMSP/PPP blends (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 34 (1996), S. 1925-1934 
    Details
    ISSN: 0887-6266
    Keywords: poly(1-trimethylsilyl-1-propyne) ; poly(1-phenyl-1-propyne) ; blends ; sorption ; hydrocarbons ; sorption models ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Propane and n-butane sorption in blends of poly(1-trimethylsilyl-1-propyne) (PTMSP) and poly(1-phenyl-1-propyne) (PPP) have been determined. Solubilities of propane and n-butane increased as the PTMSP content in the blends increased. This result is consistent with the higher free volume of PTMSP-rich blends and the better thermodynamic compatibility between PTMSP and these hydrocarbons. Propane and n-butane sorption isotherms were well described by the dual-mode model for sorption in glassy polymers. PTMSP/PPP blends are strongly phase-separated, heterogeneous materials. A noninteracting domain model developed for sorption in phase-separated glassy polymer blends suggests that sorption in the Henry's law regions (i.e., the equilibrium, dense phase of the blends) is consistent with the model. However, Langmuir capacity parameters in the blends are lower than predicted from the domain model, suggesting that the amount of nonequilibrium excess free volume associated with the Langmuir sites depends on blend composition. © 1996 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...