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  • Agrobacterium tumefaciens  (1)
  • Anthomyiidae  (1)
  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Choices and Consequences of Oviposition by Hylemya (Delia) Sp. (Diptera: Anthomyiidae) (1998)
    Springer
    Journal of insect behavior 11 (1998), S. 371-381 
    Details
    ISSN: 1572-8889
    Keywords: oviposition behavior ; host selection ; offspring performance ; predispersal seed predation ; Anthomyiidae ; Hylemya ; Delia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hylema sp. 5 females oviposit on the undersides of sepals of developing buds of both Ipomopsis aggregata and Polemonium foliosissimum. Eggs deposited on the latter are significantly more likely to be fully protected by the sepal than are eggs deposited on the former. Unexposed eggs have a significantly greater likelihood of successfully developing to the larval stage than do exposed eggs. The difference in frequency of egg exposure on the two plant species can be attributed to differences in sepal morphology: I. aggregata sepals are significantly narrower than those of P. foliosissimum. The hypothesis that females preferentially oviposit on larger flowers was unconfirmed by a manipulative choice experiment. Plants differing in the size of their flowers were potted together and presented to Hylemya in arrays in the field. Flowers of the larger-flowered pair were no more likely to be oviposited on than flowers of the smaller-flowered pair. However, there were significant negative correlations between the corolla length and the percentage of flowers laid on per day at each of two sites. There was also a significant positive correlation between the corolla width and the percentage of flowers laid on at one site. Thus females appear to be using some measure of flower morphology, or a correlated trait, in making oviposition decisions. The degree to which Hylemya is making suboptimal choices between host plant species is discussed and requires further examination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020902714225
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic transformation through the use of hyperhydric tobacco meristems (1996)
    Springer
    Molecular breeding 2 (1996), S. 73-80 
    Details
    ISSN: 1572-9788
    Keywords: Agrobacterium tumefaciens ; chimera ; microprojectile bombardment ; rolC ; vitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Exposed shoot meristems from normal and hyperhydric (vitrified) tobacco, Nicotiana tabacum, were bombarded with gold particles either coated with plasmid DNA containing neomycin phosphotransferase (NPTII), rolC and β-glucuronidase (GUS) genes (plasmid pGA-GUSGFrolC) or left uncoated. Meristems bombarded with uncoated particles were co-cultivated with Agrobacterium tumefaciens strain EHA 101 harboring the binary vector pGA-GUSGFrolC. Whole-plant transformants were produced from 4 of 40 hyperhydric meristems bombarded with uncoated particles followed by co-cultivation with A. tumefaciens. One transgenic plant was obtained from 40 normal, non-hyperhydric meristems treated. Transformation was verified by growth on kanamycin-containing medium, GUS assays, PCR, and Southern analysis. The plants tested through Southern analysis appeared to have 2 or more copies of the transgene insert. Seeds obtained from self-pollination of these transgenic plants segregated 3:1 or 15:1 (kanamycin resistant:sensitive) when germinated on medium containing 100 mg/l kanamycin, indicating transfer of foreign genes through the sexual cycle. Whole-plant transformants were not produced from 50 normal tobacco meristems bombarded with plasmid-coated gold particles and not exposed to engineered A. tumefaciens, but 1 plant of 60 bombarded hyperhydric meristems produced transgenic roots, the result of a chimera. We suggest that hyperhydric meristems are more readily transformed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171353
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