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  • 1
    Electronic Resource
    Electronic Resource
    Aflatoxins in the autopsy brain tissue of children in Nigeria (1995)
    Springer
    Mycopathologia 132 (1995), S. 35-38 
    Details
    ISSN: 1573-0832
    Keywords: Brain ; Aflatoxin ; Children ; Nigeria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Autopsy brain (cerebrum) specimens from 18 kwashiorkor children and 19 children who had died from a variety of other diseases, at the Obafemi Awolowo Teaching Hospital complex, Ile-Ife, Nigeria, were analysed for the presence of aflatoxins using high-performance liquid chromatography. Aflatoxins were detected in 81%, 15 specimens in each group. More than one type of aflatoxin was detected in 14 (37.8%) of all the specimens. Aflatoxin B1 and its reversible metabolite, aflatoxicol, were detected in 11 brain specimens of patients with kwashiorkor and 6 of those who died of other miscellaneous diseases; out of these 6, two died from measles and its complications. The frequent detection of aflatoxins in the brains of these children and sometimes in multiple forms may suggest that aflatoxins are stored in the brain tissue which could be related to the lipophilic nature of these compounds. These findings also suggest that although many children in the tropics are exposed to aflatoxins, the accumulation of aflatoxin B1 and aflatoxicol in the brains of kwashiorkor children may be a result of an impaired metabolism of these compounds by these children.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01138602
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  • 2
    Electronic Resource
    Electronic Resource
    Superoxide dismutase 1: Identification of a novel mutation in a case of familial amyotrophic lateral sclerosis (1996)
    Springer
    Human genetics 〈Berlin〉 98 (1996), S. 48-50 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mutation analysis of the superoxide dismutase gene SOD1 in a familial case of amyotrophic lateral sclerosis revealed a T→C transition at codon 151 of exon 5. This mutation results in the substitution of an isoleucine for a threonine. It appears to affect formation of dimers of the protein and is the most C-terminal amino acid change in SOD1 described to date.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050157
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of Bax, Bcl-2, Waf-1, and PCNA gene products in an immortalized human endothelial cell line undergoing p53-mediated apoptosis (1997)
    Springer
    Apoptosis 2 (1997), S. 442-454 
    Details
    ISSN: 1573-675X
    Keywords: Apoptosis ; endothelial ; p53
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Transfection of the wild-type p53 gene into an immortalized human endothelial cell line (ECV-304) by recombinant adenoviral delivery resulted in high level expression of the wild-type p53 protein and induction of apoptosis. Increases in the number of apoptotic cells were observed within 12 h after infection of ECV-304 cells with recombinant p53 adenovirus, as deter-mined by the appearance of internucleosomal DNA fragmentation ladders and by TUNEL and electron microscopic analyses. Control cells infected with a β-galactosidase recombinant adenovirus exhibited little or no increase in apoptosis over uninfected cells. The expression of Waf-1 and Bax gene products were in-creased substantially in apoptotic ECV-304 cells as determined by Northern blot, reverse transcription-PCR and immunoblotting analyses. Lesser increases in the expression of the PCNA gene were detected in ECV-304 cells undergoing apoptosis. Both control and apoptotic ECV-304 cells did not express detectable levels of Bcl-2 mRNA or protein in Northern blotting and immunoblotting analyses, respectively. The data suggest a role for the Bax gene product in p53-mediated apoptosis of endothelial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026418010549
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  • 4
    Electronic Resource
    Electronic Resource
    Two-dimensional gel analysis of apoptosis-specific p53 isoforms induced by 2-methoxyestradiol in human lung cancer cells (1998)
    Springer
    Apoptosis 3 (1998), S. 421-430 
    Details
    ISSN: 1573-675X
    Keywords: Apoptosis ; non-small cell lung carcinoma ; p53 ; phosphorylation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The natural metabolic byproduct of estradiol, 2-methoxyestradiol (2-MeOE2), induces apoptosis in human lung cancer cells by a p53-dependent mechanism. The expression of wild-type p53 isoforms was investigated in H1299 non-small cell lung carcinoma cells induced into apoptosis by 2-MeOE2. H1299 cells lack endogenous p53 and undergo predominantly a G1 arrest when infected with a recombinant wild-type p53 adenovirus. However, when H1299 cells transfected with p53 were treated with 2-MeOE2, they underwent rapid and extensive apoptosis. H1299 cells expressing mutant his273 p53 were unaffected by 2-MeOE2, indicating a dependence of 2-MeOE2-mediated apoptosis on the presence of a functional p53. Analysis of wild-type p53 phosphoisoforms in H1299 cells by two-dimensional gel electrophoresis revealed that 2-MeOE2 induced a unique group of acidic p53 isoforms. Although most of the wild-type p53 in untreated H1299 cells migrated as at least five diffuse species with isoelectric points from pH 5.5–6.3, as many as nine additional forms migrating toward the acidic region with pI values from 4.4–5.3 were detected in 2-MeOE2-treated apoptotic cells. Two other agents known to induce apoptosis, vinblastine and actinomycin D, induced a similar pattern of acidic p53 species as that observed for 2-MeOE2. The results indicated that the induction of apoptosis in H1299 cells by 2-MeOE2 is dependent on the upregulation of specific p53 isoforms. Identification of the specific p53 phosphoisoforms induced by MeOE2 will be an important step in understanding the regulation and function of p53 in apoptosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009610603068
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  • 5
    Electronic Resource
    Electronic Resource
    Induction and alternative splicing of the Bax gene mediated by p53 in a transformed endothelial cell line (1999)
    Springer
    Apoptosis 4 (1999), S. 109-114 
    Details
    ISSN: 1573-675X
    Keywords: Bax ; cell type-specific ; p53 ; reverse transcription-PCR.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Overexpression of the normal p53 protein in tumor cell lines is known to induce apoptosis and a potential mediator of this response is the apoptotic inducer, Bax. The expression of Bax mRNA products were investigated in the ECV-304 endothelial cell tumor line and primary human umbilical vein endothelial cells (HUVEC) that were induced to overexpress the p53 protein. Induction of p53 in ECV-304 and HUVEC cells was mediated by infection with a p53 recombinant adenovirus (AdCMV-p53). The expression of Bax transcripts in p53-induced cells was investigated by reverse-transcription polymerase chain reaction (RT-PCR). The Baxα mRNA species was detected in both ECV-304 and HUVEC cells. Surprisingly, Baxα expression was reduced several-fold in ECV-304 endothelial cells overproducing p53 and no change in Baxα was detected in HUVEC cells after induction of p53. However, the Baxδ spliced transcript was observed to be induced by p53 in the ECV-304 tumor cell line. Baxα was the predominant species expressed in normal human endothelial cells but, in contrast to the immortalized ECV-304 endothelial cell line, induction of p53 failed to alter the expression of Baxα or to induce any other Bax transcripts. HUVEC cells were more resistant to p53, since at least 80% of the HUVEC cell population survived the overexpression of p53 after 24 h of infection with AdCMV-p53. An ECV-304-derived cell line (DECV) resistant to p53-mediated apoptosis did not show any changes in expression of Bax mRNA products, even in the presence of high levels of p53. ECV-304 endothelial cells that expressed the Baxδ species underwent apoptosis much more rapidly and more extensively after induction of p53, suggesting that the Baxδ species enhances p53-mediated apoptosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009618811038
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  • 6
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    Superoxide dismutase 1: Identification of a novel mutation in a case of familial amyotrophic lateral sclerosis (1996)
    Springer
    Details
    Publication Date: 1996-05-30
    Print ISSN: 0340-6717
    Electronic ISSN: 1432-1203
    Topics: Biology , Medicine
    Published by Springer
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