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  • Articles  (2)
  • ADP-glucose pyrophosphorylase  (1)
  • Immunocytochemistry  (1)
  • Brassica napus
  • Somaclonal variation
  • Springer  (2)
  • 1995-1999  (2)
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  • Articles  (2)
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  • Springer  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Adenosine diphosphate glucose pyrophosphorylase genes in wheat: differential expression and gene mapping (1995)
    Springer
    Planta 197 (1995), S. 1-10 
    Details
    ISSN: 1432-2048
    Keywords: ADP-glucose pyrophosphorylase ; Gene ex ; pression (Agp1, Agp2) ; Hexaploid wheat-Starch synthesis ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA clone representing the large (shrunken-2) subunit of ADP-glucose pyrophosphorylase (AGP; EC 2.7.7.27) has been isolated from a cDNA library prepared from developing grain of hexaploid wheat (Triticum aestivum L., cv. Chinese Spring). The 2084-bp cDNA insert contains an open reading frame of 1566 nucleotides and primer-extension analysis indicated that the 5′ end is 10 nucleotides shorter than the mRNA. The deduced protein contains 522 amino acids (57.8 kDa) and includes a putative transit peptide of 62 amino acids (6.5 kDa). The similarity of the deduced protein to the small subunit of AGP and to other AGP genes from plants and microorganisms is discussed. Northern hybridisation shows that the Agp1 genes (encoding the small subunit in the wheat endosperm) and the Agp2 genes (encoding the large subunit in the wheat endosperm) are differentially expressed in the wheat grain. Transcripts from both gene sets accumulate to high levels in the endosperm during grain development with the majority of the expression in the endopsperm rather than the embryo and pericarp layers. Although enzyme activity is detected in developing grains prior to 10 d post anthesis, only the Agp1 genes are active at this time (the Agp2 genes are not expressed until 10 d post anthesis). The possibility that the enzyme expressed during early grain development is a homotetramer of small subunits is discussed. The Agp1 and Agp2 genes are arranged as triplicate sets of single-copy homoeoloci in wheat. The Agp2 genes are located on the long arms of chromosomes 1A, 1B and 1D, about 80 cM from the centromere. The Agp1 genes have been mapped to a position just distal to the centromere on the long arms of chromosomes 7A, 7B and 7D.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239933
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Presence of Locusta diuretic hormone in endocrine cells of the ampullae of locust Malpighian tubules (1996)
    Springer
    Cell & tissue research 285 (1996), S. 331-339 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Diuretic hormone ; Endocrine cells ; Midgut ; Malpighian tubules ; Bioassay ; Immunocytochemistry ; Locusta migratoria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. This is an investigation of an endocrine cell type in the midgut of the migratory locust Locusta migratoria. This cell type is found in the posterior region of the midgut and is especially common in the ampullae through which Malpighian tubules drain into the gut at the midgut-hindgut junction. Strong Locusta diuretic hormone-like immunoreactivity in these cells was colocalized with FMRFamide- and substance P-like immunoreactivities. At the ultrastructural level, immunoreactivity for Locusta diuretic hormone was found in spherical granules (mean diameter of 450 nm), the contents of which showed variable electron density. Fractionation of a methanolic extract of the ampullae by reversed-phase high performance liquid chromatography revealed the presence of two peaks of Locusta diuretic hormone-like immunoreactive material, both of which stimulate cyclic AMP production by isolated Malpighian tubules. The more hydrophobic material is most likely Locusta diuretic hormone, which has the same retention time when chromatographed under identical conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050650
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