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  • Springer  (73)
  • National Academy of Sciences  (5)
  • 1995-1999  (78)
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Year
  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 209-212 
    ISSN: 1570-7458
    Keywords: Popillia japonica ; Scarabaeidae ; herbivory ; induced response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1476-5535
    Keywords: Keywords: raloxifene; glucuronidation; Streptomyces sp NRRL 21489
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Raloxifene, also known as Evista®, has recently been approved for the prevention of osteoporosis. Three glucuronidated compounds: raloxifene-6-glucuronide, raloxifene-27-glucuronide, and raloxifene-6,27-diglucuronide are known metabolites of raloxifene in man. Reference standards of the three glucuronides were needed for clinical trials. Although chemical routes exist to make the two mono-glucuronides, these routes were unable to provide material to meet the needs of clinical trial standards. No chemical route existed to synthesize the di-glucuronide. A bioconversion process using the microorganism Streptomyces sp NRRL 21489 was identified and scaled up. The biotranformation products were prepared in a tank fermentation, purified, and characterized by UV, LC/MS and NMR spectroscopy.
    Type of Medium: Electronic Resource
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  • 13
    ISSN: 1573-0417
    Keywords: laminated sediments ; varves ; palaeoceanography ; palaeolimnology ; palaeoclimatology ; backscattered electron imagery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Conventional high resolution studies of varved sediments are able to identify clastic and biogenic laminae, but are often unable to resolve the nature of fine-scale lamination contained therein. This intra-annual signal provides us with the highest potential resolution from the sedimentary record and can be resolved using scanning electron microscopy (SEM). Six case studies from lacustrine and marine settings are presented to illustrate the combination of clastic and biogenic fabric types typically found in laminated sediments. Clastic laminae fabrics include those which originate through grain settling and those which are ‘event deposits’. The correct identification of event deposits is essential if varves are to be used chronologically. SEM-based biogenic laminae fabric studies have identified seasonal faunal successions where individual laminae may be less than 100 μ thick and most recently, deep chlorophyll maxima (DCM) summer diatom floras, providing an insight into seasonal scale processes. High resolution lamina fabric studies can provide a basis for generating records of seasonal and inter-annual variability, thus contributing to our understanding of lacustrine and marine processes and palaeoenvironmental interpretation.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Leber’s hereditary optic neuropathy (LHON) is a maternally inherited disorder of the optic nerves. It has been proposed that the specific mutations in the mitochondrial DNA (mtDNA) that are associated with LHON require and X-chromosomally encoded permissive factor in order to become expressed. This would explain both the preponderance of male patients and the fact that most carriers of specific mtDNA mutations remain unaffected. Although linkage studies have been negative so far, the existence of such a factor has not been ruled out. We investigated the genealogical data of 24 large LHON pedigrees and concluded that the presumed X-linked factor would be recessively inherited and that at least 57% of the affected females would be heterozygous. Therefore, these females must be the victim of nonrandom X-chromosomal inactivation (skewed lyonization). However, analysis of X-chromosomal methylation patterns in 16 LHON-affected females revealed substantial skewing in only 15%–20% of cases, which is not significantly different from the patterns in 49 controls. Moreover, we found the frequency of LHON in daughters of affected heterozygous females to be twice to three times as high as in daughters of unaffected heterozygous females, which cannot be explained by an X-chromosomally inherited factor. We conclude that the results of our investigations do not support the hypothesis that LHON is a digenic disease with an X-linked factor being the main cause of loss of vision in the presence of relevant mtDNA mutations.
    Type of Medium: Electronic Resource
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  • 15
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Single-strand conformational polymorphisms (SSCP) of the connexin32 gene were analyzed in 121 patients possibly affected by Charcot-Marie-Tooth (CMT) disease. The 121 patients were selected from 443 possible CMT/HNPP (hereditary neuropathy with liability to pressure palsies) patients based on genetic linkage to Xq13.1, absence of the 17p12 duplication and deletion, and absence of point mutations in PMP22 and P0. We found five new mutations at nucleotides 105 (C-T), 316 (C-G), 321 (C-T), 328 (T-C), and 657 (G-C), and three mutations at nucleotide 126 (C-T), 249 (G-A), and 477 (G-A) previously described in other unrelated families. The nucleotide changes resulted in seven amino-acid substitutions and one premature stop codon.
    Type of Medium: Electronic Resource
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  • 16
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Leber's hereditary optic neuropathy (LHON) is a maternally inherited disorder of the optic nerves. It has been proposed that the specific mutations in the mitochondrial DNA (mtDNA) that are associated with LHON require and X-chromosomally encoded permissive factor in order to become expressed. This would explain both the preponderance of male patients and the fact that most carriers of specific mtDNA mutations remain unaffected. Although linkage studies have been negative so far, the existence of such a factor has not been ruled out. We investigated the genealogical data of 24 large LHON pedigrees and concluded that the presumed X-linked factor would be recessively inherited and that at least 57% of the affected females would be heterozygous. Therefore, these females must be the victim of nonrandom X-chromosomal inactivation (skewed lyonization). However, analysis of X-chromosomal methylation patterns in 16 LHON-affected females revealed substantial skewing in only 15%–20% of cases, which is not significantly different from the patterns in 49 controls. Moreover, we found the frequency of LHON in daughters of affected heterozygous females to be twice to three times as high as in daughters of unaffected heterozygous females, which cannot be explained by an X-chromosomally inherited factor. We conclude that the results of our investigations do not support the hypothesis that LHON is a digenic disease with an X-linked factor being the main cause of loss of vision in the presence of relevant mtDNA mutations.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 247 (1997), S. 11-16 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This contribution can only be a small collection of ideas and experiences from my (personal) publishing point of view. The subject area assigned is very generic and there are indeed many developments going on, so I had to be very selective and restrictive, while avoiding redundancies as much as I could. I have included some metaphors and paradigms, such as the shift from print publications to electronic information. The traditional role of publishers in the information chain is compared with the new opportunities that electronic publishing is offering now and may offer in the foreseeable future. From DTP to DTD, unplugged and unbundled information, linearity, appropriateness, packaging and customizing, filters, intelligent agents, quality, integrity and authenticity are some of the items hidden in the text. The overall conclusion is: the Internet still can learn a lot from print...!
    Type of Medium: Electronic Resource
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  • 18
    ISSN: 1573-8787
    Keywords: explicitly locally stratified ; deductive databases ; logic programming ; magic sets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract We give a simple transformation from normal programs with no stratification (local, weak, modular, etc.) into a subclass of the locally stratified programs, called Explicitly Locally Stratified (ELS) programs, for which there are efficient evaluation techniques. One set of predicates are generated for the true tuples and a different set of predicate are generated for the true and undefined tuples. A similar transformation is given that incorporates a magic sets like transformation. Previous approaches to magic sets transformations of unstratified programs either restricted the class of sips used or generated a program that required special treatment of the magic sets predicates. Our transformation does not suffer from these flaws.
    Type of Medium: Electronic Resource
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  • 19
    ISSN: 1432-0649
    Keywords: PACS: 42.55.Rz; 42.55.Xi; 42.60.Gd
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: 4 laser and this produced 1.5-ns pulses of 7 kW peak power at a repetition rate of 20 kHz.
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  • 20
    ISSN: 1573-2614
    Keywords: Measurement techniques: pulse oximetry ; Monitoring: oxygen ; Equipment: pulse oximeters ; computers ; Oxygen: saturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine
    Notes: Abstract We have developed a simple system for internal validation of oximetry data collected over many hours from the Nellcor N-200 pulse oximeter (Nellcor, Inc., Hayward, CA). This system uses signals from the oximeter alone and a validation algorithm that is based in a computer connected to the oximeter. Unlike other validation systems, this system does not require connections to other monitors. The system was tested on 10 acutely ill newborns in an intensive care nursery over 16 hr of continuous recording for each infant (birthweight, 2.50±0.73 kg; age, 3.4±3.2 days). Oximetry data were accepted as valid using the new system if they surpassed a minimum level of quality (empirically derived, and equal to a 60% fractional success in pulse detection). The validated oximetry data were compared to data obtained using a conventional “compared to the electrocardiogram (ECG)” algorithm. For the new and the conventional algorithms, the distributions of validated SpO2 percents were nearly identical, with data rejection rates of 28.9% for the new system and 37.3% for the conventional system. In the newborns, the new system was used to demonstrate that as the mean saturations decreased, there were striking increases in variability about the reported mean saturation (p 〈 0.001). While variability in infant SpO2 is a well-known phenomenon, the amount seen here was unexpected. For example, the range of true saturations frequently recorded was quite wide at a reported mean SpO2 of 90% (from 81 to 94%; but, the range was only from 92 to 98% at a mean SpO2 of 96%). These findings demonstrate the usefulness of the new system and, if substantiated in more detailed studies, have important implications for the use of pulse oximeters to assess oxygenation in newborns.
    Type of Medium: Electronic Resource
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