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  • Gene clusters  (2)
  • Climate  (1)
  • Springer  (3)
  • Molecular Diversity Preservation International (MDPI)
  • 1995-1999  (3)
  • 1
    ISSN: 1617-4623
    Keywords: Cytochrome P450 genes ; Drosophila melanogaster ; Gene clusters ; Multigene family ; Insecticide resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twelve cytochrome P450 cDNA fragments were cloned fromDrosophila melanogaster by reverse transcriptase/PCR (RT/PCR) using degenerate oligonucleotide primers. The corresponding genes belong to several subfamilies of the CYP4 and CYP9 P450 families. Only two of these genes,Cyp4d1 andCyp4d2, have previously been described.In situ hybridization of each of the cDNA fragments showed two clusters of genes; one near the tip of theX chromosome and the other on the left arm of chromosome2. Interestingly the latter cluster comprises widely divergent genes belonging both to the CYP9 and CYP4 families and also to the CYP6 family (Cyp6a2). Putative allelic variants of several of the genes were found in different insecticide-resistant and -susceptible strains (Hikone R, Haag 79 and Oregon R). The identification of these genes and alleles will allow us to clarify the involvement of P450s in xenobiotic metabolism and will facilitate a genetic analysis of P450 functions in insects.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Key words Cytochrome P450 genes ; Drosophila melanogaster ; Gene clusters ; Multigene family ; Insecticide resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Twelve cytochrome P450 cDNA fragments were cloned from Drosophila melanogaster by reverse transcriptase/PCR (RT/PCR) using degenerate oligonucleotide primers. The corresponding genes belong to several subfamilies of the CYP4 and CYP9 P450 families. Only two of these genes, Cyp4d1 and Cyp4d2, have previously been described. In situ hybridization of each of the cDNA fragments showed two clusters of genes; one near the tip of the X chromosome and the other on the left arm of chromosome 2. Interestingly the latter cluster comprises widely divergent genes belonging both to the CYP9 and CYP4 families and also to the CYP6 family (Cyp6a2). Putative allelic variants of several of the genes were found in different insecticide-resistant and -susceptible strains (Hikone R, Haag 79 and Oregon R). The identification of these genes and alleles will allow us to clarify the involvement of P450s in xenobiotic metabolism and will facilitate a genetic analysis of P450 functions in insects.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5052
    Keywords: Climate ; Potential vegetation ; Cluster analysis ; Principal component analysis ; Temperature ; Precipitation ; Spain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Monthly precipitation and monthly mean temperature records from 255 meteorological stations in Central Spain (Spanish Sistema Central) were analyzed by cluster and principal component analyses. Classification separated 14 groups according to altitude, geographical distribution, as well as the combination of rainfall and temperature. The first cluster emerged as significantly higher and colder than the second. Then, two ordinations were carried out: one for the 14 groups extracted from the classification and a second for the total station pool. The second ordination was compared with potential natural vegetation data taken around each station. The first ordination summarized the principal climatic characteristics of the Spanish Sistema Central: its behaviour is that of a typical Mediterranean mountainous territory combining summer aridity and variation of temperature with altitude and the influence of winter winds. The ordination of all stations reflected a thermal, rainfall and summer aridity gradient. Meteorological stations situated at the highest altitude or with highest precipitation records characterized by scrub communities or pine woods and Quercus pyrenaica forests, appeared well-separated along the first two axes. Only the most thermophilous Q. rotundifolia associations could be clearly separated. The fourth axis was useful in clarifying some vegetation overlap of deciduous and sclerophyllous forests, along an east-west summer aridity gradient. Nomenclature: Tutin et al. (1964-1980) for plants; Rivas-Martínez (1987) and Rivas-Martínez et al. (1987) for syntaxa.
    Type of Medium: Electronic Resource
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