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  • 1
    Electronic Resource
    Electronic Resource
    Microbial plankton across Drake Passage (1996)
    Springer
    Polar biology 16 (1996), S. 613-622 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We determined biomass and activity of microbial plankton across the Polar Front (PF) in Drake Passage during January 1994. Temperature was around 0°C south and between 3 and 5°C north of the PF. Both biomass and activities of microorganisms were significantly lower in the Antarctic waters south of the PF than in the sub-Antarctic waters north of it. Thus, values of chlorophyll a, integrated between 0 and 200 m, reached 150 mgm−2 north, but only 25 mg m−2 south of the PF. Likewise, bacteria varied between 1014 and 4×1013 cells m−2. However, the abundance of heterotrophic nanoflagellates was extremely low throughout Drake Passage (around 3×1010 cells m−2). Bacterial doubling times were long (mean of 25 days). Bacterivory was estimated from the abundance of predators and prey and from temperature. The grazing impact on bacterioplankton biomass was insignificant (less that 0.05% per day) and low on bacterial heterotrophic production (15% per day). Neither biomass nor the activities of microorganisms were found to increase at the PF. The microbial food web was uncoupled and the bacteria did not seem to be controlled by predation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02329059
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  • 2
    Electronic Resource
    Electronic Resource
    Possible etiologic agents for toxic oil syndrome: Fatty acid esters of 3-(N-phenylamino)-1,2-propanediol (1995)
    Springer
    Archives of environmental contamination and toxicology 28 (1995), S. 259-264 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract The etiologic agent(s) that was responsible for the 1981 toxic oil syndrome [TOS] epidemic in Spain has not been identified. Liquid chromatography combined with atmospheric pressure ionization tandem mass spectrometry was used for the analysis of oils associated with TOS. Analyses focused on measuring 3-(N-phenylamino)-1,2-propanediol [PAP], the 3-oleyl ester of PAP [MEPAP], and the 1,2-di-oleyl ester of PAP [DEPAP]. DEPAP and MEPAP were found more frequently and at higher concentrations in TOS case-associated oils than in control oils with odds ratios of 13.7 (95% CI 5.0–38) and 21.9 (95% 6.1–78), respectively. Other fatty acid esters of PAP are also likely to be present in the TOS case-associated oils. More significantly, DEPAP and MEPAP were found in aniline-denatured rapeseed oil refined at ITH, the oil refining company with the clearest link to TOS cases, yet these PAP esters were not detected in unrefined aniline-denatured samples of rapeseed oil delivered to ITH. These results show that the esters of PAP were products of the ITH refining process and were not formed spontaneously during storage. PAP esters were not detected in samples of other aniline-denatured rapeseed oils that were refined elsewhere, and which were not associated with illness. These findings provide strong support for the hypothesis that one or more of the fatty acid esters of PAP were the etiologic agents for TOS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217625
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of phototrophic sulfur bacteria through the analysis of lmwRNA band patterns (1998)
    Springer
    Archives of microbiology 170 (1998), S. 269-278 
    Details
    ISSN: 1432-072X
    Keywords: Key words Phototrophic sulfur bacteria ; Chromatiaceae ; Identification ; lmwRNA ; 5S RNA ; tRNA ; Taxonomic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several phototrophic sulfur bacteria were identified preliminarily through the analysis of the low-molecular-weight RNA fraction (lmwRNA) of bacterial cells. This fraction includes the ribosomal 5S RNA and several transfer RNAs. These molecules were separated by high-resolution electrophoresis in polyacrylamide gels, and the resulting band patterns were used as fingerprints for the identification of the organisms. We examined a large number of well-characterized reference strains together with a broad range of purple sulfur bacterial isolates from freshwater and marine environments. A cluster analysis was run using the similarity matrix calculated from the band patterns. Despite the shortcomings of the method, close relatives were clustered together yielding a number of groups consistent with the phylogenetic arrangement established through the analyses of a few available 16S rRNA gene sequences. Thus, the classification obtained gives further support to rearrangement of the group as the analyses of 16S rRNA gene sequences had previously suggested. We conclude that the analysis of lmwRNA band patterns is a rapid and simple tool for grouping and preliminarily identifying new isolates of phototrophic sulfur bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050642
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  • 4
    Electronic Resource
    Electronic Resource
    Pentachlorophenol and Pentachloroanisole in Oil Samples Associated with the Toxic Oil Syndrome (1999)
    Springer
    Bulletin of environmental contamination and toxicology 62 (1999), S. 1-7 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001289900833
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  • 5
    Electronic Resource
    Electronic Resource
    Increased rate of nondisjunction in sex cells derived from low-quality semen (1998)
    Springer
    Human genetics 〈Berlin〉 102 (1998), S. 129-137 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The relationship between chromosomal nondisjunction and semen quality was studied in two groups of males who differ highly in their semen quality: 12 individuals with low-quality semen caused by varicocele, and 8 subjects with high-quality semen, selected from sperm donors for in vitro fertilization. Chromosomal nondisjunction was inferred from the rate of disomy found in mature sperm cells. To determine the rate of disomy, we applied fluorescence in situ hybridization using satellite-specific probes for chromosomes 1, 15, 18, X and Y. In sperm cells of males with low-quality semen, the mean rate of disomy for each of the autosomes and of hetero-disomy for the sex chromosomes (XY) was significantly higher than that observed in the high-quality semen samples: more than 15-fold higher for chromosomes 1 and 15, and 7-fold higher for chromosomes 18 and XY. Yet, the homo-disomy rate for each of the sex chromosomes (XX and YY) was almost the same in both types of semen. The large discrepancy between the low- and high-quality semen in the rate of sex chromosome hetero-disomy versus the similar rate of homo-disomy strongly suggests that the abnormal chromosomal segregation in meiocytes of males with low-quality semen resulted from chromosomal nondisjunction at the first meiotic division. The results indicate that men showing poor semen quality are at an increased risk for meiotic nondisjunction, similar to women at the end of their reproductive years.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050665
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  • 6
    Electronic Resource
    Electronic Resource
    Identification and characterization of mutations in patients with holocarboxylase synthetase deficiency (1999)
    Springer
    Human genetics 〈Berlin〉 104 (1999), S. 143-148 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Holocarboxylase synthetase deficiency (HCS) is an autosomal recessive disorder characterized by metabolic ketoacidosis, abnormal urine organic metabolites, and dermatitis. These symptoms are improved by pharmacological doses of biotin. In this study, we have analyzed seven patients with HCS deficiency found in European and Middle Eastern countries by using reverse transcription/polymerase chain reaction/single-stranded conformation polymorphism and a sequencing analysis. Although we had previously reported that two mutations were frequent in Japanese patients, no frequent mutations were found in the patients analyzed in this study. Seven novel mutations were identified in the cDNA of the patients; these included three missense mutations, two single-base deletions that resulted in a termination codon, a three-base in-frame deletion, and a 68-bp deletion. A new polymorphism C1121T was also identified in four alleles. A transient expression study demonstrated that the HCS activities of three missense mutations and one amino acid deletion were 1%–14% that of wild-type cDNA; in contrast, the activities of the two single-base deletions followed by a termination codon and Asp571Asn were nearly undetectable. These data suggest that a variety of mutations is responsible for decreasing HCS activity and that the aspartate residue at amino acid position 571 may be crucial for the catalytic activity of HCS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050927
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  • 7
    Electronic Resource
    Electronic Resource
    Comparison of different methods for the determination of the water content and the dry mass correction factor in various plant samples (1998)
    Springer
    Fresenius' journal of analytical chemistry 360 (1998), S. 601-604 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The water content was measured by microwave-, oven-, and freeze-drying in different vegetables like beans, corn, potato, alfalfa, garlic, and onions. Apart from beans and bean shells, all procedures resulted in similar values. Oven-drying seemed to be less suitable for beans. In samples which were in equilibrium with the laboratory environment for a longer time, the correction factor to dry mass was determined using a freeze-drying device, infrared-, microwave- and oven-drying. All the procedures yielded similar values for this factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050765
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  • 8
    Electronic Resource
    Electronic Resource
    Microbial plankton across Drake Passage (1996)
    Springer
    Polar biology 16 (1996), S. 613-622 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We determined biomass and activity of microbial plankton across the Polar Front (PF) in Drake Passage during January 1994. Temperature was around 0°C south and between 3 and 5°C north of the PF. Both biomass and activities of microorganisms were significantly lower in the Antarctic waters south of the PF than in the sub-Antarctic waters north of it. Thus, values of chlorophyll a, integrated between 0 and 200 m, reached 150 mg m-2 north, but only 25 mg m-2 south of the PF. Likewise, bacteria varied between 1014 and 4×1013 cells m-2. However, the abundance of heterotrophic nanoflagellates was extremely low throughout Drake Passage (around 3×1010 cells m-2). Bacterial doubling times were long (mean of 25 days). Bacterivory was estimated from the abundance of predators and prey and from temperature. The grazing impact on bacterioplankton biomass was insignificant (less that 0.05% per day) and low on bacterial heterotrophic production (15% per day). Neither biomass nor the activities of microorganisms were found to increase at the PF. The microbial food web was uncoupled and the bacteria did not seem to be controlled by predation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003000050096
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  • 9
    Electronic Resource
    Electronic Resource
    MrK86: A 'Not-So-Dark' Galaxy (1998)
    Springer
    Astrophysics and space science 263 (1998), S. 151-154 
    Details
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have studied the velocity field of the Blue Compact Dwarf galaxy Mrk86 using an Hα Fabry-Perot image and 14 long-slit optical spectra. From the ionized gas velocities measured we have found that Mrk86 follows a solid-body rotation law with a central angular velocity of 34 ± 5 km s-1 kpc-1. The comparison of the modeled rotation curve with our velocity data indicates a clear dominance of the stellar mass component over the dark matter. Related with the galaxy most intense star forming knot, a very strong gradient steeping is observed. Finally, we give kinematic evidence for the Mrk86-C expanding bubble.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002185522181
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  • 10
    Electronic Resource
    Electronic Resource
    NIR Properties of the UCM Star-Forming Galaxies (1998)
    Springer
    Astrophysics and space science 263 (1998), S. 147-150 
    Details
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have obtained near-infrared (JK) imaging for 67 star-forming galaxies from the Universidad Complutense de Madrid survey. Aperture photometry at one and three disk-scale lengths have been performed on the J, K and r-band images. By comparing the r - J, J - K colors and equivalent widths of Hα with evolutionary synthesis models we derive the mass of newly formed stars, which is typically a 1 per cent of the galaxy stellar mass. The galaxy stellar masses obtained range between 3×109 and 3×1011 M⊙. The distribution of burst strengths and specific star formation rates indicate a low level of star formation activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002133505342
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