ISSN:
1573-4927
Keywords:
MX1 GENE
;
WILD MICE
;
IFN-ALPHA/BETA
;
INFLUENZA VIRUS
;
DNA SEQUENCE
;
PCR-RFLV
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The mouse Mx1 gene encodes an interferon(IFN)-inducible nuclear protein and confers resistanceto influenza virus infection. The standard laboratorymouse strains all carry the Mx1- allele andare susceptible to influenza virus. In this study, severalmouse strains established from wild mice were tested todetermine their Mx1+ or Mx1-allele status with polymerase chain reaction-restrictionfragment length variation (PCR-RFLV), sequence analysis, reversetranscription (RT)-PCR, and immunofluorescence staining.All of the mouse strains originating from wild mice werefound uniformly to carry the Mx1+ allele.Therefore, it is conceivable that the Mx1+allele in wild populations serves a function againstsome pathogens related to orthomyxoviruses. The PCR-RFLVand sequence analysis allowed us to classify theMx1+ alleles of the laboratory and wild-origin mouse strainsinto distinct classes. RT-PCR and immunofluorescencestaining demonstrated that the Mx1 transcripts andproteins were induced by IFN-alpha/beta in macrophages from wild mouse species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018741312058
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