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  • Springer  (373)
  • Copernicus
  • PANGAEA
  • 1995-1999  (385)
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  • 1
    ISSN: 1420-9071
    Keywords: Glutathione ; free radicals ; antioxidants ; oxidant stressors ; buthionine ; menadione
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only inO. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect inO. danica were judged comparable to those found in animal cells whereas the resultsE. coli were not entirely equivalent. TheO. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Growth hormone (GH) exerts its regulatory functions in controlling metabolism, balanced growth and differentiated cell expression by acting on specific receptors which trigger a phosphorylation cascade, resulting in the modulation of numerous signalling pathways dictating gene expression. A panel of five monoclonal antibodies was used in mapping the presence and somatic distribution of the GH receptor by immunohistochemistry in normal and neoplastic tissues and cultured cells of human, rat and rabbit origin. A wide distribution of the receptor was observed in many cell types. Not all cells expressing cytoplasmic GH receptors displayed nuclear immunoreactivity. In general, the relative proportion of positive cells and intensity of staining was higher in neoplastic cells than in normal tissue cells. Immunoreactivity showed subcellular localisation of the GH receptor in cell membranes and was predominantly cytoplasmic, but strong nuclear immunoreaction was also apparent in many instances. Intense immunoreactivity was also observed in the cellular Golgi area of established cell lines and cultured tissue-derived cells in exponential growth phase, indicating cells are capable of GH receptor synthesis. The presence of intracellular GH receptor, previously documented in normal tissues of mostly animal origin, is the result of endoplasmic reticulum and Golgi localisation. Heterogeneity of immunoreactivity was found in normal and neoplastic tissue with a variable range of positive cells. The nuclear localisation of immunoreactivity is the result of nuclear GH receptor/binding protein, identically to the cytosolic and plasma GH-binding protein, using a panel of five monoclonal antibodies against the GH receptor extracellular region. The expression of GH receptors, not only on small proliferating tumour cells such as lymphocytes, but also on well differentiated cells including keratinocytes, suggests that GH is necessary not only for differentiation of progenitor cells, but also for their subsequent clonal expansion, differentiation and maintenance.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1327
    Keywords: Key words Superoxide dismutase ; Manganese enzyme ; Crystal structure ; Metalloprotein ; DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract  The three-dimensional structure of the manganese-dependent superoxide dismutase (MnSOD) from Escherichia coli has been determined by X-ray crystallography at 2.1 Å resolution. The protein crystallizes with two homodimers in the asymmetric unit, and a model comprising 6528 protein atoms (residues 1–205 of all four monomers), four manganese ions and 415 water molecules has been refined to an R factor of 0.188 (R free 0.218). The structure shows a high degree of similarity with other MnSOD and FeSOD enzymes. The Mn centres are 5-coordinate, trigonal bipyramidal, with His26 and a solvent molecule, probably a hydroxide ion, as apical ligands, and His81, Asp167 and His171 as equatorial ligands. The coordinated solvent molecule is linked to a network of hydrogen bonds involving the non-coordinated carboxylate oxygen of Asp167 and a conserved glutamine residue, Gln146. The MnSOD dimer is notable for the way in which the two active sites are interconnected and a "bridge" comprising His171 of one monomer and Glu170 of the other offers a route for inter-site communication. Comparison of E. coli MnSOD and FeSOD (a) reveals some differences in the dimer interface, (b) yields no obvious explanation for their metal specificities, and (c) provides a structural basis for differences in DNA binding, where for MnSOD the groove formed by dimerization is complementary in charge and surface contour to B-DNA.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 1996-06-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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  • 5
    Publication Date: 1998-01-28
    Print ISSN: 0018-2222
    Electronic ISSN: 1432-119X
    Topics: Biology , Medicine
    Published by Springer
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  • 6
    Publication Date: 2023-06-27
    Keywords: 16 km ENE Cape Roberts; Cape Roberts Project; Comment; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; off Cape Roberts, Ross Sea, Antarctica; Sampling/drilling ice; δ13C, carbonate; δ18O, carbonate; δ18O, water
    Type: Dataset
    Format: text/tab-separated-values, 28 data points
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  • 7
    Publication Date: 2023-06-27
    Keywords: 16 km ENE Cape Roberts; Calcareous fossils; Calcium carbonate; Cape Roberts Project; Chert; Clay minerals; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; Heavy minerals; Intrusive, igneous rock; Iron carbonate, siderite; Kalifeldspar; Metamorphite; Mica; off Cape Roberts, Ross Sea, Antarctica; Opaque minerals; Plagioclase; Point counting with SEM/EDAX; Porosity; Pyroxene; Quartz; Sampling/drilling ice; Sedimentary rock; Sulfate; Volcanic glass; Volcanite
    Type: Dataset
    Format: text/tab-separated-values, 171 data points
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  • 8
    Publication Date: 2023-06-27
    Keywords: 16 km ENE Cape Roberts; Calcium carbonate; Cape Roberts Project; Comment; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; Energy dispersive X-ray analysis, EDAX; Iron carbonate, siderite; Magnesium carbonate, magnesite; Manganese carbonate, rhodochrosite; off Cape Roberts, Ross Sea, Antarctica; Sampling/drilling ice; Strontium carbonate, strontianite
    Type: Dataset
    Format: text/tab-separated-values, 120 data points
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  • 9
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    Unknown
    PANGAEA
    In:  Supplement to: Fielding, Christopher R; Baker, Julian C; Woolfe, Ken; Howe, John; Lavelle, Mark (1998): Reassessment of the Miocene-Quaternary boundary in CRP-1, Cape Roberts Project, Antarctica. Terra Antartica, 5(3), 425-426, hdl:10013/epic.28334.d001
    Publication Date: 2023-07-10
    Description: During the course of the 1997 drilling campaign, lithostratigraphic boundaries were assigned to the CRP-l core on the basis of perceived changes in lithology. The geologically most important boundary in the core, between the Miocene and overlying Quaternary sections, was placed at 43.55 mbsf. This horizon was described in the core logs (Cape Roberts Science Team, 1998) as a contact between muddy, finegrained sandstone (which were assigned a Lower Miocene age based on diatom biostratigsaphy) and overlying diamictons containing Quaternary diatoms. This boundary is a major unconformity, recognisable on seismic reflection records. As such, it has considerable significance in the ongoing scientific analysis of the drillcore. During a re-examination of the core, focusing on the archive half held at the Antarctic Geology Research Facility of the Florida State University at Tallahassee; the authors noted that the core across the published boundary (43.55 mbsf) did not show any lithological change, but logged a sharp contact between dark olive grey, muddy sandstone and overlying diamicton at 43.15 mbsf (Fig. l). We suggest, therefore, that the core log in appendix l of Cape Roberts Science Team (1998) is misleading over this interval. In order to test the veracity of the suggested boundary revision, a series of thin-sections was examined and point-counted for framework grain abundances.
    Keywords: 16 km ENE Cape Roberts; Calcite; Cape Roberts Project; Chert; Claystone; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; Epoch; Grain size, mean radius; Kalifeldspar; Micrite; off Cape Roberts, Ross Sea, Antarctica; Opaque minerals; Plagioclase; Porosity; Pyroxene; Quartz; Sampling/drilling ice; Sorting description; Thin section analysis/measurements; Volcanic glass; Volcanite
    Type: Dataset
    Format: text/tab-separated-values, 75 data points
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  • 10
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    Unknown
    PANGAEA
    In:  EPIC3Terra Antartica, Bremerhaven, PANGAEA, 5(3), pp. 425-426
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: PANGAEA Documentation , notRev
    Format: application/pdf
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