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  • Benzophenone imine derivatives  (1)
  • Endothelial  (1)
  • Springer  (2)
  • American Physical Society
  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 17 (1995), S. 33-39 
    ISSN: 1573-0603
    Keywords: Airway circulation ; Bronchial artery ; Cell culture ; Endothelial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methods are described for the isolation of endothelial cells from sheep bronchial artery by treatment with collagenase. Cells obtained from the perfusate of the collagenase-treated vessel are cultured. Identified by their cobblestone morphology, endothelial cell colonies of approximately 50 cells are selected by a cloning cylinder and subcultured using trypsin. Endothelial cells are characterized by the formation of vessel-like tubes on basement membrane and by incorporation of DiI-acetylated-low density lipoprotein.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chiral stationary phases ; Benzophenone imine derivatives ; Amino acid esters ; Enantiomer separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The liquid-chromatographic separation of the enantiomers of amino acid esters as benzophenone Schiff-base derivatives on polysaccharide-derived chiral stationary phases (CSPs) is described. The performance of Chiralcel OF was superior to that of the other CSPs for resolution of benzophenone imine derivatives of amino acid ethyl and methyl esters. The enantiomers of most of the amino acid esters examined as their benzophenone imine derivatives were resolved to baseline on Chiralcel OF. The L-(−) enantiomers of all the analytes were preferentially retained on Chiralcel OF. The resolution of several imine derivatives of amino acid esters was investigated, as was the effect of eluent composition on the resolution of amino acid ethyl esters as their benzophenone imine derivatives.
    Type of Medium: Electronic Resource
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