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  • Molecular markers  (54)
  • Springer  (54)
  • American Institute of Physics (AIP)
  • American Physical Society
  • Copernicus
  • 1995-1999  (54)
Collection
Keywords
Publisher
  • Springer  (54)
  • American Institute of Physics (AIP)
  • American Physical Society
  • Copernicus
  • Wiley-Blackwell  (1)
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Association of a major groat oil content QTL and an acetyl-CoA carboxylase gene in oat (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 884-894 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oat ; Oil content ; Acetyl-CoA carboxylase ; Molecular markers ; Candidate gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Oat groats are unique among cereals for the high level and the embryo-plus-endosperm localization of lipids. Genetic manipulation of groat quality traits such as oil is desired for optimizing the value of oat in human and livestock diets. A locus having a major effect on oil content in oat groats was located on linkage group 11 by single-factor analysis of variance, simple interval mapping and simplified composite interval mapping. A partial oat cDNA clone for plastidic acetyl-CoA carboxylase (ACCase), which catalyzes the first committed step in de novo fatty acid synthesis, identified a polymorphism linked to this major QTL. Similar QTL and ACCase locus placements were obtained with two recombinant inbred populations, ‘Kanota’בOgle’ (KO) and ‘Kanota’בMarion’ (KM), containing 137 and 139 individual lines, respectively. By having a common parent these populations provide biological replication of the results in that significant genomic regions should be evident in analyses of multiple cross combinations. The KO population was mapped with 150 RFLP loci distributed over the genome and was grown in five diverse environments (locations and years) for measurement of groat oil content. The KM population was mapped with 60 RFLP loci and grown in three environments. The QTL linked to AccaseA on linkage group 11 accounted for up to 48% of the phenotypic variance for groat oil content. These results provide strong support for the hypothesis that ACCase has a major role in determining groat oil content. Other QTLs were identified in both populations which accounted for an additional 10–20% of the phenotypic variance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051147
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro androgenesis and segregation distortion in Brassica napus L.: spontaneous versus colchicine-doubled lines (1997)
    Springer
    Plant cell reports 16 (1997), S. 464-468 
    Details
    ISSN: 1432-203X
    Keywords: Key words Brassicaceae ; Microspore cultures ; Haploid plants ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 750 plantlets were regenerated from 1,400 embryos produced through microspore cultures from one F1 plant of the cross `Darmor-bzh' × `Yudal'. Fifty-three percent of the regenerants were evaluated by flow cytometric analysis, which revealed that 31% were spontaneous diploid (SD), 63% were still haploid and the remaining 6% plants had other ploidy levels. Available segregation data (266 markers) produced on this androgenic progeny were used to study the interference between segregation distortion and the mode of chromosome doubling of androgenc lines. On the basis of the present results it is not possible to conclude that distortions are peculiar to one type of regenerated plant; only a difference in the intensity of the bias might be assumed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092767
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro androgenesis and segregation distortion inBrassica napus L.: spontaneous versus colchicine-doubled lines (1997)
    Springer
    Plant cell reports 16 (1997), S. 464-468 
    Details
    ISSN: 1432-203X
    Keywords: Brassicaceae ; Microspore cultures ; Haploid plants ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 750 plantlets were regenerated from 1,400 embryos produced through microspore cultures from one F1 plant of the cross ‘Darmor-bzh’ x ‘Yudal’. Fifty-three percent of the regenerants were evaluated by flow cytometric analysis, which revealed that 31% were spontaneous diploid (SD), 63% were still haploid and the remaining 6% plants had other ploidy levels. Available segregation data (266 markers) produced on this androgenic progeny were used to study the interference between segregation distortion and the mode of chromosome doubling of androgenc lines. On the basis of the present results it is not possible to conclude that distortions are peculiar to one type of regenerated plant; only a difference in the intensity of the bias might be assumed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092767
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 219-227 
    Details
    ISSN: 1432-2242
    Keywords: Key words DNA polymorphisms ; Genetic distances ; Molecular markers ; Yield prediction ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The challenge to maize breeders is to identify inbred lines that produce highly heterotic hybrids. In the present study we surveyed genetic divergence among 13 inbred lines of maize using DNA markers and assessed the relationship between genetic distance and hybrid performance in a diallel set of crosses between them. The parental lines were assayed for DNA polymorphism using 135 restriction fragment length polymorphisms (RFLPs) and 209 amplified-fragment polymorphisms (AFLPs). Considerable variation among inbreds was detected with RFLP and AFLP markers. Moreover AFLPs detect polymorphisms more efficiently in comparison to RFLPs, due to the larger number of loci assayed in a single PCR reaction. Genetic distances (GDs), calculated from RFLP and AFLP data, were greater among lines belonging to different heterotic groups compared to those calculated from lines of the same heterotic group. Cluster analysis based on GDs revealed associations among lines which agree with expectations based on pedigree information. The GD values of the 78 F1 crosses were partioned into general (GGD) and specific (SGD) components. Correlations of GD with F1 performance for grain yield were positive but too small to be of predictive value. The correlations of SGDs, particularly those based on AFLP data, with specific combining-ability effects for yield may have a practical utility in predicting hybrid performance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050730
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  • 5
    Electronic Resource
    Electronic Resource
    A molecular genetic map of cassava (Manihot esculenta Crantz) (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 431-441 
    Details
    ISSN: 1432-2242
    Keywords: Key words Cassava ; Molecular markers ; Genetic mapping ; Polyploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM 2177-2’, elite cassava cultivars from Nigeria and Colombia, respectively. The map consists of 20 linkage groups spanning 931.6 cM or an estimated 60% of the cassava genome. Average marker density is 1 per 7.9 cM. Since the mapping population is an F1 cross between heterozygous parents, with unique alleles segregating from either parent, a second map was constructed from the segregation of 107 RFLPs, 50 RAPDs, 1 microsatellite, and 1 isoenzyme marker from the male parent. Comparison of intervals in the male-and female-derived maps, bounded by markers heterozygous in both parents, revealed significantly less meiotic recombination in the gametes of the female than in the male parent. Six pairs of duplicated loci were detected by low-copy genomic and cDNA sequences used as probes. Efforts are underway to saturate the cassava map with additional markers, to join the male- and female-derived maps, and to elucidate genome organization in cassava.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050580
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  • 6
    Electronic Resource
    Electronic Resource
    RFLP and RAPD markers linked to the rosy leaf curling aphid resistance gene (Sd1) in apple (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 528-533 
    Details
    ISSN: 1432-2242
    Keywords: Key words Apple ; Dysaphis devecta ; Insect resistance ; Marker-assisted selection ; Molecular markers ; Plant breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sd 1 is a dominant gene for resistance to biotypes 1 and 2 of the rosy leaf curling aphid, Dysaphis devecta Wlk., which can cause economic damage to apple trees. This report describes the identification of three RFLP and four RAPD markers linked to Sd 1 in a cross between the D. devecta susceptible variety ‘Prima’ (sd 1 sd 1) and the resistant variety ‘Fiesta’ (Sd 1 sd 1). Potted trees were artificially infested in the glasshouse, and the ratio of resistant:susceptible plants supported the hypothesis that the resistance was under the control of a single dominant gene. The position of the gene was mapped to a single locus on a ‘Fiesta’ chromosome, within 2 cM of three tightly linked RFLP markers (MC064a, 2B12a and MC029b); the four RAPD markers were located further away (between 13 and 46 cM). This is the first report of molecular markers for an aphid resistance gene in tree fruit crops. The potential application of these markers in a marker-assisted resistance breeding programme is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050447
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular markers linked to genes affecting plant height in wheat using a doubled-haploid population (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 933-940 
    Details
    ISSN: 1432-2242
    Keywords: Key words Plant height ; Molecular markers ; QTL ; Wheat ; Doubled-haploid lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Plant height in wheat (Triticum aestivum L. em Thell) is known to be under polygenic control. Crosses involving genes Rht-B1 and Rht-D1, located on chromosomes 4BS and 4DS, respectively, have shown that these genes have major effects. Two RFLP loci were found to be linked to these two genes (Xfba1-4B with Rht-B1 and Xfba211-4D with Rht-D1) by genotyping a population of F1-derived doubled-haploid lines [‘Courtot’ (Rht-B1b+Rht-D1b)בChinese Spring’]. Using a well-covered molecular marker map, we detected three additional regions and one interaction influencing plant height. These regions, located on chromosome arms 4BS (near the locus Xglk556-4B), 7AL (near the locus Xglk478-7A) and 7BL (near the locus XksuD2-7B) explained between 5% and 20% of the variability for this trait in this cross. The influence of 2 loci from chromosome 4B (Xfba1-4B and Xglk556-4B) suggests that there could be a duplication of Rht-B1 on this chromosome originating from Cv ‘Courtot’. Moreover, an interaction effect between loci from chromosome arms 1AS (near the locus Xfba393-1A) and 1BL (near the locus Xcdo1188-1B) was comparable to or even higher than those of the Rht-B1b and Rht-D1b alleles. A model including the main effects of the loci from chromosomes 4B and 4D (Xfba1-4B, Xglk556-4B and Xfba211-4D) and the interaction effect between Xfba393-1A and Xcdo1188-1B is proposed, which explains about 50% of the variation in plant height. The present results are discussed in relation to those obtained using nullisomic or substitution lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050823
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic analysis of the dwarfing gene Rht8 in wheat. Part II. The distribution and adaptive significance of allelic variants at the Rht8 locus of wheat as revealed by microsatellite screening (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1110-1120 
    Details
    ISSN: 1432-2242
    Keywords: Key words Adaptability ; Dwarfing gene (Rht8) ; Microsatellite ; Molecular markers ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Wheat microsatellite WMS 261 whose 192-bp allele has been shown to be diagnostic for the commercially important dwarfing gene Rht8 was used to screen over 100 wheat varieties to determine the worldwide spread of Rht8. The results showed Rht8 to be widespread in southern European wheats and to be present in many central European wheats including the Russian varieties ‘Avrora’, ‘Bezostaya’ and ‘Kavkaz’. Rht8 appears to be of importance to South European wheats as alternative giberellic acid (GA)-insensitive dwarfing genes do not appear to be adapted to this environment. The very successful semi-dwarf varieties bred by CIMMYT, Mexico, for distribution worldwide have been thought to carry Rht8 combined with GA-insensitive dwarfing genes. Additional height reduction would have been obtained from pleiotropic effects of the photoperiod-response gene Ppd1 that is essential to the adaptability of varieties bred for growing under short-winter days in tropical and sub-tropical areas. The microsatellite analysis showed that CIMMYT wheats lack Rht8 and carry a WMS 261 allelic variant of 165 bp that has been associated with promoting height. This presumably has adaptive significance in partly counteracting the effects of other dwarfing genes and preventing the plants being too short. Most UK, German and French wheats carry an allelic variant at the WMS 261 locus with 174 bp. This could be selected because of linkage with the recessive photoperiod-sensitive ppd1 allele that is thought to offer adaptive significance northern European wheats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050846
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  • 9
    Electronic Resource
    Electronic Resource
    Inheritance of parthenogenesis in Poa pratensis L.: auxin test and AFLP linkage analyses support monogenic control (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 74-82 
    Details
    ISSN: 1432-2242
    Keywords: Key words Apomixis ; Auxin test analysis ; Bulked segregant analysis ; Gene mapping ; Kentucky bluegrass ; Parthenogenesis ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Gametophytic apomixis in Kentucky bluegrass (Poa pratensis L.) involves the parthenogenetic development of unreduced eggs from aposporic embryo sacs. Attempts to transfer the apomictic trait beyond natural sexual barriers require further elucidation of its inheritance. Controlled crosses were made between sexual clones and apomictic genotypes, and the parthenogenetic capacity of (poly)diploid hybrids was ascertained by the auxin test. A bulked segregant analysis with RAPD and AFLP markers was then used to identify a genetic linkage group related to the apomictic mode of reproduction. This approach enabled us to detect both an AFLP marker located 6.6 cM from the gene that putatively controls parthenogenesis and a 15.4-cM genomic window surrounding the target locus. A map of the P. pratensis chromosome region carrying the gene of interest was constructed using additional RAPD and AFLP markers that co-segregated with the parthenogenesis locus. Highly significant linkage between parthenogenesis and a number of AFLP markers that also appeared to belong to a tight linkage block strengthens the hypothesis of monogenic inheritance of this trait. If a single gene is assumed, apomictic polyploid types of P. pratensis would be simplex for a dominant allele that confers parthenogenesis, and this genetic model would be further supported by the bimodal distribution of the degree of parthenogenesis exhibited in the (poly)diploid progenies from sexual x apomictic matings. The molecular tagging of apomixis in P. pratensis is an essential step towards marker-assisted breeding and map-based cloning strategies aimed at investigating and manipulating its mode of reproduction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050868
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  • 10
    Electronic Resource
    Electronic Resource
    Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1248-1255 
    Details
    ISSN: 1432-2242
    Keywords: Key words Zea mays L. ; Genetic relationship ; Molecular markers ; DNA-fingerprinting ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  DNA-based fingerprinting technologies have proven useful in genetic similarity studies. RFLP is still most commonly used in the estimation of genetic diversity in plant species, but the recently developed PCR-based marker techniques, RAPDs, SSRs and AFLPs, are playing an increasingly important role in these investigations. Using a set of 33 maize inbred lines we report on a comparison of techniques to evaluate their informativeness and applicability for the study of genetic diversity. The four assays differed in the amount of polymorphism detected. The information content, measured by the expected heterozygosity and the average number of alleles, was higher for SSRs, while the lowest level of polymorphism was obtained with AFLPs. However, AFLPs were the most efficient marker system because of their capacity to reveal several bands in a single amplification. In fact, the assay efficiency index was more than ten-fold higher for AFLPs compared to the other methods. Except for RAPDs, the genetic similarity trees were highly correlated. SSR and AFLP technologies can replace RFLP marker in genetic similarity studies because of their comparable accuracy in genotyping inbred lines selected by pedigree. Bootstrap analysis revealed that, in the set of lines analysed, the number of markers used was sufficient for a reliable estimation of genetic similarity and for a meaningful comparison of marker technologies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051017
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