Publication Date:
1998-03-21
Description:
The mechanism by which mammalian circadian clocks are entrained to light-dark cycles is unknown. The clock that drives behavioral rhythms is located in the suprachiasmatic nucleus (SCN) of the brain, and entrainment is thought to require induction of genes in the SCN by light. A complementary DNA subtraction method based on genomic representational difference analysis was developed to identify such genes without making assumptions about their nature. Four clones corresponded to genes induced specifically in the SCN by light, all of which showed gating of induction by the circadian clock. Among these genes are c-fos and nur77, two of the five early-response genes known to be induced in the SCN by light, and egr-3, a zinc finger transcription factor not previously identified in the SCN. In contrast to known examples, egr-3 induction by light is restricted to the ventral SCN, a structure implicated in entrainment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Morris, M E -- Viswanathan, N -- Kuhlman, S -- Davis, F C -- Weitz, C J -- New York, N.Y. -- Science. 1998 Mar 6;279(5356):1544-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9488654" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antisense Elements (Genetics)
;
Blotting, Southern
;
Circadian Rhythm
;
Cloning, Molecular
;
Cricetinae
;
DNA, Complementary
;
DNA-Binding Proteins/*genetics
;
Early Growth Response Protein 3
;
*Gene Expression Regulation
;
*Genes, fos
;
*Light
;
Male
;
Mesocricetus
;
Nuclear Receptor Subfamily 4, Group A, Member 1
;
Polymerase Chain Reaction
;
RNA, Messenger/analysis/genetics
;
Receptors, Cytoplasmic and Nuclear
;
Receptors, Steroid
;
Suprachiasmatic Nucleus/*physiology
;
Transcription Factors/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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