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Digitale Medien

Prenatal development of the integument in Delphinidae (Cetacea: Odontoceti) (1995)

Meyer, Wilfried ; Neurand, Klaus ; Klima, Milan

New York, NY : Wiley-Blackwell

Journal of Morphology 223 (1995), S. 269-287

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ISSN: 0362-2525

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: The prenatal development of epidermis, dermis, and hypodermis was studied in embryos of different ago of two delphinid species (Stenella attenuata, Delphinus delphis), using light and transmission electron microscopical methods. The delphinid embryo is covered by a multilayered tissue formed by four different epidermal generations (periderm, stratum intermedium-I, str. intermedium-II, str. spinosum) produced by the str. basale. The first layer appears at about 40-50 mm of body length, the second type (s.i.-I) about 60-160 mm, and the third type (s.i.-II) is present at 160-500 mm. The first spinosal cells are produced at 225-260 mm body length; thenceforth, the epidermis increases continuously in thickness. Epidermal ridge formation begins about 400-mm body length. The development of the dermis is characterized by the early production of thin connective tissue fibers (40- 70-mm body length) and simultaneously the cutaneuous muscle matures in structure. Vascular development intensifies between embryos of 150-225 mm, and collagen production increases markedly in fetuses of 225-260-mm length. These events are paralledled by an increase in dermal thickness. The first elastic fibers can be recognized in the skin from the abdomen at about 600-mm body length. The development of the hypodermis is marked by very rapid and constantly progressing growth, beginning about 60-mm body length. The first typical fat cells appear in animals of 360-400 mm. Regional differences are obvious for all skin layers with regard to the flippers, where structural maturation proceeds more rapidly than in dorsal or abdominal regions. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 9 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jmor.1052230305

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Digitale Medien

Tubulin gene expression during growth and maturation of leaves with different developmental patterns (1995)

Hellmann, Andreas ; Meyer, Claudius U. ; Wernicke, Wolfgang

New York, NY : Wiley-Blackwell

Cell Motility and the Cytoskeleton 30 (1995), S. 67-72

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ISSN: 0886-1544

Schlagwort(e): Nicotiana ; Hordeum ; microtubule ; cell differentiation ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: Changes in the tubulin-protein and -poly(A)+RNA contents were monitored by means of Western and Northern blot analyses, respectively, during growth and maturation of leaves of a dicotyledonous (tobacco) and monocotyledonous (barley) plant. It was recently argued from immunofluorescence and preliminary biochemical data that the density of microtubular networks and concomitantly the tubulin content are distinctly reduced after cessation of cell growth in leaves [Jung et al., 1993]. The results presented now confirm and extend this view. There appeared to be clear differences between the monocot and the dicot: (1) the loss of tubulin during leaf development was much slower in the dicot than in the monocot leaves (within months instead of days); (2) the degree of loss was more dramatic in the monocot leaf and only very low threshold levels of tubulin were retained in fully differentiated tissues; and (3) the loss of tubulin in the monocot leaf tissue appeared to be correlated with the decrease in the mRNA content, whereas the high level of tubulin-RNA in fully differentiated or even almost senescent dicot leaves indicated a gene expression control at the posttranscriptional level.The comparatively rapid and very distinct tubulin-protein and -RNA disappearance during development of the monocot leaf tissues confirm at the molecular level that differentiation proceeds much faster and is much more determinative in these leaves, as was postulated from histological and physiological data. The differences in the behaviour of the microtubular cytoskeleton perhaps even reflect the differences in the ability of the differentiated leaf cells to dedifferentiate, i.e., to establish new sets of microtubules and to reenter the mitotic cell cycle, e.g., during would response, tumour induction or in vitro culture. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/cm.970300108

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3

Digitale Medien

1,25(OH)2D3-dependent regulation of calbindin-D28k mRNA requires ongoing protein synthesis in chick duodenal organ culture (1995)

Meyer, Julia ; Galligan, Michael A. ; Jones, Glenville ; [weitere]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 58 (1995), S. 315-327

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ISSN: 0730-2312

Schlagwort(e): calbindin-D28k ; 1,25-dihydroxyvitamin D3 ; messenger RNA ; organ culture ; polymerase chain reaction ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Organ culture of 19-day-old chick embryo duodena was utilized to evaluate the mechanism of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-dependent calbindin-D28k (CaBP) expression. Duodenal CaBP and 1,25(OH)2D3 receptor (VDR) expression were assessed by Western blot analysis, while CaBP and VDR mRNA levels were determined by Northen blot analysis. In untreated duodena, both VDR protein and mRNA were present, while CaBP protein and mRNA were undetectable. Treatment of cultured duodena with 25 nM 1,25(OH)2D3 resulted in detectable CaBP mRNA after 4 h which continued to increase during a 24 h time period. Under these conditions, localization of [3H-1β]1α,25(OH)2D3 in duodenal chromatin is rapid (≤ 30 min). Thus, the delayed accumulation of detectable CaBP mRNA cannot be explained by slow nuclear binding of 1,25(OH)2D3. The inclusion of 1.6 μM actinomycin D in the organ culture partially inhibited the 1,25(OH)2D3-regulated increase in CaBP mRNA, which implies that there is a transcriptional component involved in the increased CaBP mRNA levels. Similarly, quantitative polymerase chain reaction studies allowed the detection of CaBP pre-mRNA and mRNA sequences 1 h after hormone treatment, suggesting that CaBP gene transcription is initiated rapidly. Treatment of cultures with 36 μM cycloheximide 1 h prior to 1,25(OH)2D3 addition resulted in superinduction of VDR mRNA levels but sharply reduced CaBP steady-state mRNA levels. This dramatic reduction in CaBP mRNA reveals that 1,25(OH)2D3-mediated CaBP expression is dependent on ongoing protein synthesis. Thus, we propose that a labile auxiliary protein or other cofactor, which may or may not be 1,25(OH)2D3-dependent, is necessary for 1,25(OH)2D3-mediated CaBP gene transcription in chick duodena.

Zusätzliches Material: 10 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcb.240580306

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Digitale Medien

Computer networked scanning electron microscope for teaching, research, and industry applications (1995)

Scott Chumbley, L. ; Meyer, Mitch ; Fredrickson, Kjell ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 32 (1995), S. 330-336

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ISSN: 1059-910X

Schlagwort(e): Scanning electron microscopy ; Teaching ; Computer ; Network ; Remote control ; Ethernet ; Internet ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: A laboratory designed for teaching the operation of a scanning electron microscope (SEM) has been developed. The laboratory makes use of a computer network to allow remote operation of the SEM. Movable teaching stations, consisting of a computer, TV monitor, and joystick control, enable students to view the image on the SEM screen, move the sample, control the basic operating parameters of the microscope, and acquire X-ray spectra. Images can also be stored on the computers for image analysis or incorporation into reports. The great advantage of the system is that it has been designed to be flexible enough to allow operation from any location that has access to the Internet. The system is relatively inexpensive and uses nonproprietary computer technology available at any computer store. While the laboratory has been designed for teaching, the concept of a multiuser SEM facility that is inexpensive and easy to install should have applications in both industrial and research settings. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jemt.1070320407

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5

Digitale Medien

The effect of adrenalectomy on the succinic dehydrogenase activity as related to nitrogen and other components of rat liver tissueThis investigation was supported in part by a research grant from the National Institutes of Health and the Wisconsin Alumni Research Foundation. (1952)

Stucki, Jacob C. ; Shipley, Elva G. ; Meyer, Roland K.

Philadelphia : Wiley-Blackwell

Journal of Cellular and Comparative Physiology 40 (1952), S. 169-182

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ISSN: 0095-9898

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Zusätzliches Material: 4 Tab.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1030400202

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6

Digitale Medien

Laminin decreases PRL and IGFBP-1 expression during in vitro decidualization of human endometrial stromal cells (1995)

Brar, A. K. ; Frank, G. R. ; Richards, R. G. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 163 (1995), S. 30-37

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: The expression of laminin, a major constituent of endometrial cell basement membranes, is increased during differentiation of human endometrial stromal cells (decidualization). To determine whether laminin plays a role in decidualization, we studied the effects of laminin substrate on the synthesis and release of prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), two major secretory proteins of decidualized stromal cells. Endometrial stromal cells were plated on laminin as well as several other extracellular matrix (ECM) proteins (types 1 and IV collagen or fibronectin) and on plastic, and cultured in media containing medroxyprogesterone acetate (MPA) and estradiol. Cells cultured on plastic or ECM proteins displayed similar morphological changes indicative of decidualization. However, the release of PRL and IGFBP-1 from cells cultured on plastic and ECM proteins (types 1 and IV collagen and fibronection) was approximately 2.1-fold and 2.8-fold greater respectively, than from cells cultured on laminin. The decrease in PRL and IGFBP-1 expression in cells cultured on laminin was not due to differences in initial cell attachment efficiency or final DNA content. In addition, laminin had no effect on the content of laminin protein or fibronectin mRNA levels, indicating that the effects of laminin on PRL and IGFBP-1 were specific. PGE2 stimulated the release of PRL and IGFBP-1 from cells cultured on laminin to levels comparable to those from cells cultured on plastic or other ECM proteins. This indicates that the decrease in PRL and IGFBP-1 release by laminin was not due to a generalized unresponsiveness. In contrast to the effects of laminin during decidualization, PRL expression was not altered by laminin in terminally differentiated decidual cells isolated at term. Our results support a role for laminin in selectively regulating PRL and IGFBP-1 gene expression during in vitro decidualization of human endometrial stromal cells. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1041630105

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Digitale Medien

Ternäre Halogenide vom Typ A3MX6. III [1, 2]. Synthese, Strukturen und Ionenleitfähigkeit der Halogenide Na3MX6 (X = Cl, Br)Professor Hans-Uwe Schuster in memoriam (1995)

Wickleder, Mathias S. ; Meyer, Gerd

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 621 (1995), S. 457-463

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ISSN: 0044-2313

Schlagwort(e): Ternary halides ; synthesis ; crystal structures ; ionic conductivity ; Chemistry ; Inorganic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Beschreibung / Inhaltsverzeichnis: Ternary Halides of the A3MX6 Type. III [1, 2]. Synthesis, Structures, and Ionic Conductivity of the Halides Na3MX6 (X = Cl, Br)The bromides Na3MBr6 crystallize with the stuffed LiSbF6-type structure (type I; M = Sm—Gd) or with the structure of the mineral cryolite (type II; M = Gd—Lu). The structure types were refined from single crystal X-ray data (Na3SmBr6: trigonal, space group R3, a = 740.8(2) pm, c = 1 998.9(8) pm, Z = 3; Na3YBr6: monoclinic, space group P21/n, a = 721.3(4) pm, b = 769.9(2) pm, c = 1 074.8(4) pm, β = 90.60(4)°, Z = 2). Reversible phase transitions from one structure to the other occur. The phase transition temperatures were determined for the bromides as well as for the chlorides Na3MCl6 (M = Eu—Lu). The refinement of both structures for one compound was possible for Na3GdBr6 (I: trigonal, space group R3, a = 737.1(5) pm, c = 1 887(2) pm, Z = 3; II: monoclinic, space group P21/n, a = 725.2(1) pm, b = 774.1(3) pm, c = 1 080.1(3) pm, β = 90.76(3)°, Z = 2). All compounds exhibit ionic conductivity of the sodium ions which decreases with the change from type I to type II. The conductivity of the bromides is always higher when compared with the respective chlorides.

Notizen: Die Bromide Na3MBr6 kristallisieren im aufgefüllten LiSbF6-Typ (Typ I; M = Sm—Gd) oder im Kryolith-Typ (Typ II; M = Gd—Lu). Die Strukturen wurden anhand von Einkristalldaten verfeinert (Na3SmBr6: trigonal, Raumgruppe R3, a = 740,8(2) pm, c = 1 998,9(8) pm, Z = 3; Na3YBr6: monoklin, Raumgruppe P21/n, a = 721,3(4) pm, b = 769,9(2) pm, c = 1 074,8(4) pm, β = 90,60(4)°, Z = 2). Beide Strukturen sind reversibel ineinander umwandelbar. Die Umwandlungstemperaturen der Bromide wurden ebenso wie die der Chloride Na3MCl6 (M = Eu—Lu) bestimmt. An Na3GdBr6 wurden beide Strukturen an Einkristallen bestimmt (I: trigonal, Raumgruppe R3, a = 737,1(5) pm, c = 1 887(2) pm, Z = 3; II: monoklin, Raumgruppe P21/n, a = 725,2(1) pm, b = 774,1(3) pm, c = 1 080,1(3) pm, β = 90,76(3)°, Z = 2). Alle Verbindungen zeigen Natrium-Ionenleitfähigkeit, die beim Übergang vom Typ I in den Typ II stark abnimmt. Die Bromide zeigen, verglichen mit den Chloriden, jeweils eine bessere Leitfähigkeit.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/zaac.19956210321

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8

Digitale Medien

Synthese und Kristallstrukturen der Ternären Selten-Erd-Chloride NaMCl4 (M = Eu - Yb, Y) (1995)

Wickleder, Mathias S. ; Meyer, Gerd

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 621 (1995), S. 546-549

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ISSN: 0044-2313

Schlagwort(e): Ternary halides ; synthesis ; crystal structures ; Chemistry ; Inorganic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Beschreibung / Inhaltsverzeichnis: Synthesis and Crystal Structures of the Ternary Rare Earth Chlorides NaMCl4 (M = Eu - Yb, Y)Single crystals of NaErCl4 were obtained from the melt of NaCl and ErCl3 (1:1 molar ratio) by slow cooling. It crystallizes in the monoclinic crystal system (space group P2/c) with the structure of α-NiWO4 with a = 632.24(9) pm, b = 759.78(9) pm, c = 674.2(1) pm, b̃ = 92.310(3)°, Z = 2. Two preparative routes to pure powder samples of the chlorides NaMCl4 are described. At room temperature, these are found to be isotypic with NaErCl4 (M = Tm - Yb; II) while the triclinic structure of NaGdCl4 is adopted with M = Gd - Ho, Y (I). Phase transitions from one structure to the other are observed for all compounds. The transition temperatures decrease with decreasing size of the ion M3+.

Notizen: Einkristalle von NaErCl4 erhält man durch langsames Abkühlen einer Schmelze von NaCl und ErCl3 (molares Verhältnis 1:1). NaErCl4-II kristallisiert monoklin (Raumgruppe P2/c) im α-NiWO4-Typ mit a = 632,24(9) pm, b = 759,78(9) pm, c = 674,2(1) pm, b̃ = 92,310(3)°, Z = 2. Zwei Wege zu reinen Pulverproben der Chloride NaMCl4 werden beschrieben. Bei Raumtemperatur sind diese für M = Tm - Yb isotyp zu NaErCl4 (II), mit M = Gd - Ho, Y kristallisieren sie in der triklinen Struktur von NaGdCl4 (I). Beide Modifikationen lassen sich reversibel ineinander umwandeln. Die Umwandlungstemperaturen werden mit abnehmendem Ionenradius von M3+ kleiner.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/zaac.19956210408

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Digitale Medien

NaEu2Cl6 und Na0,75Eu2Cl6: Gemischtvalente Chloride des Europiums mit NatriumProfessor Herbert Jacobs zum 60. Geburtstag gewidmet (1996)

Wickleder, Mathias S. ; Meyer, Gerd

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 622 (1996), S. 593-596

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ISSN: 0044-2313

Schlagwort(e): Reduced halides ; synthesis ; crystal structure ; europium ; Chemistry ; Inorganic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Beschreibung / Inhaltsverzeichnis: NaEu2Cl6 and Na0.75Eu2Cl6: Mixed Valent Chlorides of Europium with SodiumThe reaction of Na2EuCl5 with Eu metal in sealed gold tubes yields blue single crystals of NaEu2Cl6. It crystallizes with the hexagonal crystal system (space group P63/m) with a = 755.74(8) pm, c = 429.81(5) pm, Z = 1; the structure is closely related to the UCl3-type. Green single crystals of Na0.75Eu2Cl6 were first obtained as a by-product in the synthesis of Na2EuCl5 in evacuated silica tubes and may be prepared by reduction of EuCl3 with sodium. Na0.75Eu2Cl6 crystallizes isotypic to NaEu2Cl6 with a = 753.69(11) pm and c = 416.3(2) pm.

Notizen: Die Umsetzung von Na2EuCl5 mit Eu-Metall in Goldampullen führt zu blauen Einkristallen von NaEu2Cl6, einem Derivat des UCl3-Typs: Hexagonal, Raumgruppe P63/m, a = 755,74(8) pm, c = 429,81(5) pm, Z = 1. Grüne Einkristalle von Na0,75Eu2Cl6 wurden erstmals als Nebenprodukt bei der Synthese von Na2EuCl5 in evakuierten Quarzglasampullen erhalten, können jedoch auch durch gezielte Reduktion von EuCl3 mit Natrium gewonnen werden. Na0,75Eu2Cl6 ist isotyp mit NaEu2Cl6 mit a = 753,69(11) pm und c = 416,3(2) pm.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/zaac.19966220403

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Digitale Medien

Synthese und Kristallstruktur der “Fluorid-Ammoniakate“ Zr(NH3)F4 und Hf(NH3)F4 (1997)

Plitzko, Christian ; Strecker, Michael ; Meyer, Gerd

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 623 (1997), S. 79-83

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ISSN: 0044-2313

Schlagwort(e): Crystal structure ; synthesis ; ammine complexes ; fluoride ; zirconium ; hafnium ; Chemistry ; Inorganic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Beschreibung / Inhaltsverzeichnis: Synthesis and Crystal Structure of the Fluoride-Ammine Complexes Zr(NH3)F4 and Hf(NH3)F4Colourless, easily cleavable single crystals of Zr(NH3)F4 and Hf(NH3)F4 without special shape are obtained by oxidation of zirconium and hafnium metal powder with NH4HF2 (molar ratio 1 : 2) in sealed Monel ampoules at reaction temperatures of 380-450°C. The two fluorideammine complexes crystallize isotypically in the triclinic space group P1 (no. 2) with a = 601.4(1)/597.8(8) pm [Zr(NH3F4)/Hf(NH3)F4], b = 802.8(2)/800.6(12) pm, c = 862.3(2)/860.8(8) pm, α = 106.39(1)/106.19(10)°, β = 104.25(1)/104.51(9)°, γ = 106.83(1)/106.69(11)°, Z = 4. Bicapped trigonal prisms [M(NH3)F7] are connected via edges and corners to form corrugated layers that are held together by hydrogen bonding.

Notizen: Farblose, leicht spaltbare Einkristalle von Zr(NH3)F4 und Hf(NH3)F4 ohne ausgeprägten Habitus erhält man durch Oxidation von Zirconium- bzw. Hafnium-Metallpulver mit NH4HF2 (molares Verhältnis 1:2) in verschweißten Monelampullen bei Reaktionstemperaturen von 380-450°C. Die beiden Ammoniakate kristallisieren isotyp in der triklinen Raumgruppe P1 (Nr. 2) mit a = 601,4(1)/597,8(8) pm [Zr(NH3)F4/Hf(NH3)F4], b = 802,8(2)/800,6(I2) pm, c = 862,3(2)/860,8(8) pm, α = 106,39(1)/106,19(10)°, β = 104,25(1)/104,51(9)°, γ = 106,83(1)/106,69(11)°, Z = 4. Es liegen zweifach bekappte trigonale Prismen [M(NH3)F7] (M = Zr, Hf) vor, die über gemeinsame Kanten und Ecken zu gewellten Schichten verknüpft sind, die ihrerseits über Wasserstoffbrückenbindungen zusammengehalten werden.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/zaac.19976230114

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