ISSN:
1432-2048
Keywords:
Brassica napus
;
Colchicine
;
Heat shock
;
Microspore embryogenesis
;
Microtubules
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis fromBrassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction inB. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00620060
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