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  • Biochemistry and Biotechnology  (10)
  • Industrial Chemistry and Chemical Engineering  (4)
  • 1995-1999  (14)
  • 1960-1964
  • 1
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemical Engineering & Technology - CET 19 (1996), S. 315-323 
    ISSN: 0930-7516
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Intermig impellers have been postulated as very efficient for mixing highly viscous non-Newtonian fluids (such as xanthan and mycelial broths). However, no formal characterisation has been published and no fair comparisons have been made, based on accurate power drawn measurements and using equal number of impeller stages and equal diameter, if compared (for example) with the performance of Rushton turbines. Characterisation of the shape, size, and evolution of the well-mixed zones or “caverns” were correlated with power drawn, for single and dual Rushton turbines and for one- as well as two-stage Intermig unslotted impellers. Cavern evolution studies were carried out in a mixing tank (diameter=0.205 m, H/T=1.6) equipped with an accurate air bearing dynamometer. Carbopol 940 (0.25 wt.-%) was used as a model, transparent fluid. Impeller to tank diameter ratio was 0.53 for both impellers. Caverns were visualised by injecting methylene blue in the well-mixed zones. A single Rushton turbine developed larger caverns if compared with one-stage Intermig of the same diameter under power drawn below 1.5 kW m-3. At higher power drawn, both impellers behaved very similarly, reaching a limit in cavern volume of about 40% of the total liquid volume, even at very high (20 kW m03) power drawn. A similar trend characterised dual combinations: below 3 kW m-3, dual Rushtons gave larger cavern volume if compared with the performance of two-stage Intermigs. In either case, power drawn higher than 3 kW m03 was sufficient to mix more than 90% of the liquid volume. The presence or absence of the slot in the Intermig did not influence cavern development. Experiments with a smaller if compared with those obtained with the larger Intermig (D/T = 0.53).
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemical Engineering & Technology - CET 20 (1997), S. 287-292 
    ISSN: 0930-7516
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The development and optimization of the synthesis of an analogue of sperm whale oil have been carried out. The product is an ester with characteristics similar to those of natural sperm whale oil. A central composite design was applied in the synthesis of this fine chemical. The variables selected for the present study are reaction temperature, initial concentration of catalyst and working pressure. Temperature is the most significant factor in the esterification process, and its influence is positive. Pressure influence can be neglected, and the catalyst concentration has a positive influence on the process. Depending on the temperature value, the influence of the interactions can be more important than that of the other two main effects, pressure and catalyst concentration. Response surface models have been found adequate to represent the yield of ester. The commercial quality of the synthesized product is very similar to that of natural spermaceti. Because of its low cost, this synthesis process is considered, from an economical point of view, very attractive.
    Additional Material: 3 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemical Engineering & Technology - CET 19 (1996), S. 410-419 
    ISSN: 0930-7516
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: General simple criteria to predict and analyze the influence on trickle-bed reactors behavior of three phenomena, namely, plug flow deviations, external wetting efficiency and external mass transfer resistance, are developed. Criteria predictions are compared with results arising from a comprehensive mathematical model widely employed to describe trickle-bed reactors behavior and with other particular criteria reported in the literature. Present criteria applications to different sets of experimental data exemplify their practical utility for various actual situations and verify their validity. Besides, they constitute simple tools to diagnose detrimental on trickle-bed reactors operation.
    Additional Material: 4 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemical Engineering & Technology - CET 19 (1996), S. 538-542 
    ISSN: 0930-7516
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A Comparative study of different Lewis acids (metal chlorides of valences, I, II, III, IV) has been made. These catalysts are used in the synthesis of a high molecular weight ester in a stirred tank reactor using octanoic acid and n-octyl alcohol as reactants. It was found that the best efficiency of the formatted ester (n-octyl octanoate) was obtained with ferric chloride. This could be explained by the high electronegativity and the existence of free d orbitals in the Lewis acid.
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  • 5
    ISSN: 0006-3592
    Keywords: metabolic engineering ; carbon metabolism ; Escherichia coli mutants ; microbial growth ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Escherichia coli strains devoid of one or both of the two pyruvate kinase isoenzymes (PKA and PKF), were grown on minimal media in batch fermentations. The strain lacking both PKs showed a 28% decrease on its specific growth rate when compared to the wild type. However, protein and CO2 yields did not change. Using radioactive 1-C14 glucose and collecting the CO2 produced by the cultures, it was found that the mutant lacking both pyruvate kinases, metabolized glucose mainly through the pentose pathway (PP). The increased participation of the PP in glucose metabolism in this strain, was also reflected on the levels of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases.© 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:292-295, 1998.
    Additional Material: 3 Ill.
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  • 6
    ISSN: 0884-3996
    Keywords: chemiluminescence ; flow injection ; isoniazid ; solid-phase reactors ; pharmaceutical analysis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A flow injection procedure for the indirect chemiluminescent determination of isoniazid is proposed. The method is performed in a flow-injection manifold provided with a solid-phase reactor. The reactor was made from manganese dioxide physically entrapped by polymerization; the redox reaction isoniazid-manganese dioxide released Mn(II) which was monitored through its inhibitory effect on the reaction between luminol and hydrogen peroxide in presence of potassium hexacyanoferrate(III). The procedure resulted in a linear calibration graph over the range 5-15 mg/L of isoniazid with a sample throughput of 43 samples/h. The influence of foreign compounds was studied and the method was applied to determination of the drug in a pharmaceutical formulation. © 1998 John Wiley & Sons, Ltd.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 62-70 
    ISSN: 0006-3592
    Keywords: osmotic shock ; water permeability ; mixing time constant ; mathematical model ; yeast ; leukemia K562 cells ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time constant has been proposed allowing an accurate Lp estimation even though the mixing time constant is higher than the cell time constant obtained in response to a perfect shock. The estimated Lp values of human leukemia K562 cells were found to be the same whatever the mixing time constant. The Lp value of Saccharomyces cerevisiae could not be exactly estimated. However, S. cerevisiae has unexpectedly high water permeability, implying that this yeast may contain water channels in the membrane. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 62-70, 1997.
    Additional Material: 3 Ill.
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  • 8
    ISSN: 1075-2617
    Keywords: β-bend ; 310-helix ; peptide conformational analysis ; spin-labelled amino acid ; TOAC peptides ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A variety of host L-alanine homo-peptides (to the pentamer) containing one or two spin-labelled TOAC (2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid) residues were synthesized by solution methods and fully characterized. The conformational features of the terminally blocked, doubly spin-labelled-TOAC-(Ala)2-TOAC-Ala- pentapeptide were examined in the crystal state by X-ray diffraction and in solution using a combination of techniques (Fourier transform infrared, circular dichroism, cyclic voltammetry and electron spin resonance) in comparison with singly labelled shorter peptides. The 310-helical structure of the pentapeptide, promoted by the two Cα,α-disubstituted glycines under favourable experimental conditions, allows an interaction to take place between the two nitroxide TOAC side chains spaced by one turn of the helix. Taken together, these results suggest that TOAC is an excellent probe for exploring bends and helices in doubly labelled peptides.
    Additional Material: 8 Ill.
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  • 9
    ISSN: 1075-2617
    Keywords: β-amino-alcohol ; statine ; UNCAs ; vicinal tricarbonyl compounds ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: N-Urethane-protected N-carboxyanhydrides (UNCAs) are very reactives. They have been successfully used in peptide synthesis, in both solution and solid phase. We have demonstrated that UNCAs are interesting starting materials for the synthesis of various amino acid derivatives. Chemoselective reduction of UNCAs with sodium borohydride led the corresponding N-protected β amino alcohols. Reaction of UNCAs with Meldrum's acid, followed by cyclisation, yielded enantiomerially pure tetramic acid derivatives. Diastereoselective reduction of tetramic acid derivatives produced (4S,5S)-N-alkoxycarbonyl-4-hydroxy-5-alkylpyrrolidin-2-ones derived from amino acids, which after hydrolysis yielded statine and statine analogues. Tetramic acid derivatives could also be obtained by reaction of UNCAs with benzyl ethyl followed by hydrogenolytic deprotection and decarboxylation. UNCAs also reacted with phosphoranes to produce the ketophosphorane in excellent yields. Subsequent oxidation with oxone or with [bis(acetoxy)-iodol]-benzene produced vicinal tricarbonyl derivatives. These reactions usually proceeded smoothly and with high yields.
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  • 10
    ISSN: 0173-0835
    Keywords: Two-dimensional polyacrylamide gel electrophoresis ; Silver staining ; Mass spectrometry ; Proteome ; Mitochondria ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Owing to the complexity of higher eukaryotic cells, characterization of a complete proteome is likely to be difficult to achieve. However, advantage can be taken of the cell compartmentalization to build organelle proteomes, which can moreover be viewed as specialized tools to study specifically the biology and “physiology” of the target organelle. Within this frame, we report here the construction of the human mitochondrial proteome, using placenta as the source tissue. Protein identification was carried out mainly by peptide mass fingerprinting, but other methods were also used (N-terminal microsequencing, blotting). The optimization steps in two-dimensional (2-D) electrophoresis needed for proteome research are discussed. However, the relative paucity of data concerning mitochondrial proteins is still the major limiting factor in building the corresponding proteome, which should be a useful tool for researchers working on human mitochondria and their deficienciesThis work can be found on the Internet at the following address: http://www-dsv.cea.fr/MitoPick/Default.html.
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