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  • Articles  (3)
  • 12-oxo-phytodienoic acid  (1)
  • In vitro splicing  (1)
  • Key words: Biological life support  (1)
  • 1995-1999  (3)
  • 1965-1969
  • 1925-1929
  • Biology  (3)
  • Chemistry and Pharmacology
Collection
  • Articles  (3)
Source
Keywords
Publisher
Years
  • 1995-1999  (3)
  • 1965-1969
  • 1925-1929
Year
Topic
  • Biology  (3)
  • Chemistry and Pharmacology
  • 1
    Electronic Resource
    Electronic Resource
    Splicing of the mitochondrial group-II intron rl1: conserved intron-exon interactions diminish splicing efficiency (1998)
    Springer
    Current genetics 33 (1998), S. 117-123 
    Details
    ISSN: 1432-0983
    Keywords: Key words Chloroplast in vivo splicing ; Group-II intron ; In vitro splicing ; Chlamydomonas reinhardtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mitochondrial intron rI1 is a self-splicing group-II intron of algal mitochondria that can be transferred into chloroplasts from the green alga Chlamydomonas reinhardtii for in vivo investigations (Herdenberger et al. 1994). Thus, rI1 is a suitable system to compare in vitro and in vivo RNA processing. Interestingly, rI1 shows correct RNA splicing, although typical cis-acting exon-sequences (IBS2, δ) of group-II introns are lacking. In order to examine the effect of these exon-intron interactions on splicing, we introduced the endogenous mitochondrial IBS2 sequence in order to produce optimal IBS2-EBS2 base pairing. In addition, the first nucleotide of the 3′exon (δ′) was substituted to create an optimal δ-δ′ interaction. Neither of the two mutations, nor a combination of both, had any effect on the precision of the splice-site selection. Unexpectedly, introduction of IBS2 led to a reduction in the efficiency of the second splicing step in vitro but not in vivo. These findings lead us to conclude that trans-acting factors are present in vivo to optimize splicing efficiency. The possibility is discussed that these factors may, for example, stabilize tertiary intron structures that are a prerequisite for correct RNA processing. Furthermore, our data indicate that similar trans-acting factors promote correct intron splicing in chloroplasts and mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050316
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP 74), the first enzyme of the octadecanoid pathway to jasmonates (1996)
    Springer
    Plant molecular biology 31 (1996), S. 323-335 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; allene oxide synthase ; CYP74 ; octadecanoids ; jasmonate biosynthesis ; 12-oxo-phytodienoic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Allene oxide synthase, an enzyme of the octadecanoid pathway to jasmonates, was cloned from Arabidopsis thaliana as a full-length cDNA encoding a polypeptide of 517 amino acids with a calculated molecular mass of 58705 Da. From the sequence, an N-terminal transit peptide of 21 amino acids resembling chloroplast transit peptides was deduced. Three out of four invariant amino acid residues of cytochrome P450 heme-binding domains are conserved and properly positioned in the enzyme coding region, including the heme-accepting cysteine (Cys-470). Southern analysis indicated in A. thaliana only one allene oxide synthase gene to be present. While transcript levels were rapidly and transiently induced after wounding of the leaves, allene oxide synthase activity remained nearly constant at a low level of ca. 0.8 nkat per mg of protein. The cDNA encoding A. thaliana allene oxide synthase was highly expressed in bacteria giving rise to a polypeptide of the calculated molecular mass. The protein was enzymatically active, and verification of the reaction products by GC-MS showed that it was capable of utilizing not only 13-hydroperoxylinolenic acid (13-hydroperoxy-9(Z), 11(E), 15(Z)-octadecatrienoic acid), but also 13-hydroperoxylinoleic acid (13-hydroperoxy-9(Z), 11(E)-octadecadienoic acid) as substrate. The data suggest parallel pathways to jasmonates from linolenic acid or linoleic acid in A. thalina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021793
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  • 3
    Electronic Resource
    Electronic Resource
    The closed equilibrated biological aquatic system: general concept and aspects of botanical research (1997)
    Springer
    Planta 203 (1997), S. S201 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Biological life support ; Ceratophyllum (morphology ; physiology) ; Ecosystem (closed aquatic) ; Nitrate uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The Closed Equilibrated Biological Aquatic System (CEBAS) consists of four subcomponents which form a closed (artificial) aquatic ecosystem initially designed to study the long-term influence of space conditions on several successive generations of aquatic organisms. Teleost fishes and water snails in the zoological component produce CO2, ammonium ions and waste compounds which can be utilized after ammonium is oxidised in a microbial component by the botanical component consisting of a rootless, aquatic higher plant species which eliminates ions, i.e. nitrate, and produces oxygen for animal respiration. An electronic component serves as a data-acquisition and regulation device for temperature and oxygen-dependent illumination of the plant chamber. A comprehensive interdisciplinary research programme, focused around the CEBAS, is especially well developed in the field of zoology. It covers a ground laboratory and preparations for two scheduled spaceflight projects, as well as aspects of combined animal-plant food production modules for human nutrition in bioregenerative space life-support systems and for terrestrial production sites. In the botanical research programme, morphological investigations on Ceratophyllum demersum L. performed with light and electron microscopy have demonstrated a gas lacuna system which, in addition to starch grains in the plastids, might regulate the buoyancy of the plant and/or serve as a `gas skeleton'. Also, a remarkable symmetry in the arrangement of tissues was observed in stems and older leaves. The photosynthetic capacities of Ceratophyllum in the CEBAS-MINI MODULE proved to be more than sufficient for life support, and experiments on nitrate uptake into the plants showed their capacity to utilize ions from the water.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008109
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