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  • QTL  (31)
  • Springer  (31)
  • Institute of Physics
  • 1995-1999  (31)
  • 1965-1969
  • 1955-1959
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  • Springer  (31)
  • Institute of Physics
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Year
  • 1
    Electronic Resource
    Electronic Resource
    RFLP analysis of resistance to Columbia root-knot nematode derived from Solanum bulbocastanum in a BC2 population (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 572-576 
    Details
    ISSN: 1432-2242
    Keywords: Wild species ; Introgression ; Meloidogyne chitwoodi ; Gene mapping ; Potato resistance breeding ; QTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mapping of resistance toMeloidogyne chitwoodi derived from Solarium bulbocastanum is reported. A population suitable for mapping was developed as follows. A somatic hybrid of nematode-resistant S. bulbocastanum and cultivated tetraploid potato was produced. This was backcrossed to tetraploid potato, and a single resistant BC1 was selected and backcrossed again to the same recurrent tetraploid parent. The mapping population consisted of 64 BC2 progeny scored for restriction fragment length polymorphic (RFLP) markers and 62 of these were evaluated for the reproductive efficiency of race 1 of M. chitwoodi. Forty-eight polymorphic RFLP markers, originally derived from tomato and mapped in diploid cultivated potato, were assigned to 12 chromosomes of S. bulbocastanum. Of the 62 progeny screened for nematode resistance, 18 were non-hosts and four were poor hosts. The rest were highly susceptible (good hosts). Analysis of the resistance (including non-hosts and poor hosts) as both a qualitative trait and as a meristic trait on which QTL analysis was applied supported the same genetic hypothesis. Genetic control was localized solely to factor(s) lying at one end of chromosome 11. The level of expression of resistance in the S. bulbocastanum parent and the resistant portion of the BC2 was essentially the same. This fact, together with the highly significant LOD scores for one end of the chromosome-11 marker array, supports a genetic model equivalent to monogenic dominant control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224560
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  • 2
    Electronic Resource
    Electronic Resource
    Several QTLs involved in osmotic-adjustment trait variation in barley (Hordeum vulgare L.) (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 688-698 
    Details
    ISSN: 1432-2242
    Keywords: Key words Water stress ; Barley ; Osmotic adjustment ; Adaptation ; QTL ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Osmotic adjustment (OA) was previously demonstrated to be an important adaptive mechanism of drought tolerance in cereals. In order to determine which genomic regions are involved in OA variation, 187 barley (Hordeum vulgare L.) recombinant inbred lines (RILs) derived from a cross between Tadmor (drought tolerant) and Er/Apm (susceptible) were studied in a growth chamber for their OA capacity (through correlated traits and by calculation), at an early growth stage and under two water treatments (soil moisture of 14% and 100% of field capacity). The continuous distribution of the traits and their broad-sense line heritabilities, ranging from 0.04 to 0.44, indicated that OA and related traits should have a polygenic nature. A subset of 167 RILs were also genotyped using 78 RFLP, 32 RAPD and three morphological markers and a linkage map was constructed. Despite strong environmental effects acting on the traits, interval mapping and single-marker ANOVA allowed the detection of three QTLs for relative water content (RWC), four QTLs for osmotic potential (ψπ), two QTLs of osmotic potential at full turgor (ψπ100) and one QTL for osmotic adjustment at a soil moisture of 14% field capacity. For the irrigated treatment, only two QTLs were detected: one for RWC and one for ψπ100. Two chromosomal regions were involved in several OA-related trait variations and could be considered as regions controlling OA; these were present on chromosome 1 (7H) and chromosome 6 (6H), whereas other regions were specific for one trait. No major QTL was found. However, the genomic region involved in OA-related traits on chromosome 1 (7H) in barley seemed to be conserved for OA variation among cereals. Epistatic effects, with or without additive effects, acted on the traits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050790
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic dissection of height in maritime pine seedlings raised under accelerated growth conditions (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 849-858 
    Details
    ISSN: 1432-2242
    Keywords: Pinus pinaster ; Maturation ; Height ; RAPD ; QTL ; Megagametophyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Random Amplified Polymorphic DNAs (RAPDs) were used to investigate quantitative trait loci (QTL) for traits related to height growth on 126 F2 seedlings of maritime pine (Pinuspinaster Ait). The haploid megagametophyte was used to determine the maternal genotype of each F2 individual. The seedlings were raised for 2 years in a greenhouse under accelerated growth conditions consisting of intense fertilization combined with continuous light treatments. Total height was measured at different developmental stages, and height growth components were measured after the second growth period. QTLs were identified for each trait. For total height, QTLs of different developmental stages were located on distinct linkage groups. However, rather than a complete temporal change in QTL expression, our results showed that maturation may induce a progressive shift of the genetic control of height growth. This may provide an explanation for a low juvenile-mature phenotypic correlation previously reported for height. Height growth components related to the initiation (controlled by the apical meristem) and elongation of shoot cycles (controlled by the subapical meristem) were mapped to different chromosomes, suggesting that the activity of these meristems is controlled by separate genetic mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224085
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  • 4
    Electronic Resource
    Electronic Resource
    Mapping loci controlling the concentrations of erucic and linolenic acids in seed oil of Brassica napus L. (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 282-286 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica napus ; RFLP linkage map ; QTL ; Erucic acid ; Linolenic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The quality of plant oil is determined by its component fatty acids. Relatively high levels of linolenic acid reduce the oxidative stability of the oil, and high levels of erucic acid in the diet have been associated with health problems. Thus, oilseed Brassica napus cultivars with low linolenic and low erucic acid contents are highly desirable for edible oil production. In order to identify genes controlling the levels of erucic and linolenic acids, we analyzed the oil composition of 99 F1-derived doubled haploid lines from a cross between cv `Major' (high levels of erucic and linolenic acids) and cv `Stellar' (low levels of both fatty acids). A molecular marker linkage map of 199 loci for this population was used to identify quantitative trait loci (QTL) controlling oil composition. We identified two regions that accounted for nearly all of the phenotypic variation in erucic acid concentration and one region that accounted for 47% of the variation in linolenic acid concentration. The QTL associated with linolenic acid concentration mapped near a RFLP locus detected by a cDNA clone encoding an omega-3 desaturase, suggesting that the low linolenic acid content of `Stellar' may be due to a mutation in this gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050278
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  • 5
    Electronic Resource
    Electronic Resource
    RFLP analysis of resistance to Columbia root-knot nematode derived from Solanum bulbocastanum in a BC2 population (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 572-576 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Wild species ; Introgression ; Meloidogyne chitwoodi ; Gene mapping ; Potato resistance breeding ; QTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   The mapping of resistance to Meloidogyne chitwoodi derived from Solanum bulbocastanum is reported. A population suitable for mapping was developed as follows. A somatic hybrid of nematode-resistant S. bulbocastanum and cultivated tetraploid potato was produced. This was backcrossed to tetraploid potato, and a single resistant BC1 was selected and backcrossed again to the same recurrent tetraploid parent. The mapping population consisted of 64 BC2 progeny scored for restriction fragment length polymorphic (RFLP) markers and 62 of these were evaluated for the reproductive efficiency of race 1 of M. chitwoodi. Forty-eight polymorphic RFLP markers, originally derived from tomato and mapped in diploid cultivated potato, were assigned to 12 chromosomes of S. bulbocastanum. Of the 62 progeny screened for nematode resistance, 18 were non-hosts and four were poor hosts. The rest were highly susceptible (good hosts). Analysis of the resistance (including non-hosts and poor hosts) as both a qualitative trait and as a meristic trait on which QTL analysis was applied supported the same genetic hypothesis. Genetic control was localized solely to factor(s) lying at one end of chromosome 11. The level of expression of resistance in the S. bulbocastanum parent and the resistant portion of the BC2 was essentially the same. This fact, together with the highly significant LOD scores for one end of the chromosome-11 marker array, supports a genetic model equivalent to monogenic dominant control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050165
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  • 6
    Electronic Resource
    Electronic Resource
    Mapping loci controlling the concentrations of erucic and linolenic acids in seed oil of Brassica napus L. (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 282-286 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; RFLP linkage map ; QTL ; Erucic acid ; Linolenic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The quality of plant oil is determined by its component fatty acids. Relatively high levels of linolenic acid reduce the oxidative stability of the oil, and high levels of erucic acid in the diet have been associated with health problems. Thus, oilseed Brassica napus cultivars with low linolenic and low erucic acid contents are highly desirable for edible oil production. In order to identify genes controlling the levels of erucic and linolenic acids, we analyzed the oil composition of 99 F1-derived doubled haploid lines from a cross between cv ‘Major’ (high levels of erucic and linolenic acids) and cv ‘Stellar’ (low levels of both fatty acids). A molecular marker linkage map of 199 loci for this population was used to identify quantitative trait loci (QTL) controlling oil composition. We identified two regions that accounted for nearly all of the phenotypic variation in erucic acid concentration and one region that accounted for 47% of the variation in linolenic acid concentration. The QTL associated with linolenic acid concentration mapped near a RFLP locus detected by a cDNA clone encoding an omega-3 desaturase, suggesting that the low linolenic acid content of ‘Stellar’ may be due to a mutation in this gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225758
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  • 7
    Electronic Resource
    Electronic Resource
    Interval mapping of quantitative trait loci affecting NESTUR, a stem growth efficiency index of radiata pine seedlings (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1062-1068 
    Details
    ISSN: 1432-2242
    Keywords: Key words NESTUR ; Stem growth efficiency ; RAPD ; QTL ; Haploid megagametophyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  NESTUR (needle-to-stem unit rate) is a stem growth index of conifer seedlings that measures the efficiency of stemwood production per unit of needle growth, and is related to other seedling traits such as height, stem diameter, stem volume and needle volume. Quantitative trait loci (QTLs) affecting the expression of stem growth efficiency in radiata pine seedlings were investigated using a RAPD linkage map constructed from markers scored on haploid, megagametophytic DNA. Four putative QTLs were detected which accounted for 8.5–36.4% of the population variance. A search for evidence of epistasis, using both complete pairwise and conditional interactions, did not yield any statistically significant result. Over a 3-year period, seedlings with high-NESTUR marker alleles showed a superior growth performance of 17–40% for height, diameter and volume over those with low-NESTUR marker alleles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050992
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  • 8
    Electronic Resource
    Electronic Resource
    Age-related changes in the expression of QTLs for growth in radiata pine seedlings (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1053-1061 
    Details
    ISSN: 1432-2242
    Keywords: Key words RAPD ; QTL ; Age effects ; Stem growth ; Linkage map ; Haploid megagametophyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Haploid megagametophytes from a full-sib cross of Pinus radiata were used to construct a genetic linkage map for radiata pine based on random amplified polymorphic DNA (RAPD) markers. The map, which was made from 222 markers, was used to carry out a QTL analysis of growth using measurements made on seedlings from which these megagametophytes were collected at an early germination stage. Trends in the expression of QTLs for stem diameter, volume and height were compared using measurements made at 5 months of age, and at 1, 2 and 3 years of age. None of the observed trends showed complete stability, i.e. none of the putative QTL positions detected at any one age was strongly expressed at all of the four stages of measurement. However, 45% of the trends showed partial stability, i.e. putative QTLs significant at one age were also detected at a subsequent age. Trends in QTL expression with age followed one of three patterns: (1) putative QTLs at some locations showed a gradual linear increase in influence from 5 months of age and were highest at 3 years of age; (2) QTLs detected at 5 months of age gradually became less significant with age; and (3) some putative QTLs showed a curvilinear increase in effect from 5 months of age, reaching their peak expression at 1 to 2 years, and sometimes were still detected at 3 years of age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050991
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  • 9
    Electronic Resource
    Electronic Resource
    QTL for field resistance to late blight in potato are strongly correlated with maturity and vigour (1999)
    Springer
    Molecular breeding 5 (1999), S. 387-398 
    Details
    ISSN: 1572-9788
    Keywords: potato ; late blight ; QTL ; Linkage mapping ; earliness ; vigour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field resistance to Phytophthora infestans, the causal agent of foliage and tuber blight in cultivated potatoes, earliness (maturity) and vigour, were examined in a diploid segregating potato population grown in replicated trials over three consecutive growing seasons. A genetic linkage map of this population was constructed in parallel using PCR-based SSR, AFLP and CAPS markers. Analysis of the trait scores alongside the marker segregation data allowed the identification of regions of the genome which were significantly correlated with components of the respective characters. The most significant associations for all four traits were with marker alleles on potato linkage group V originating from the male (susceptible) parent. In the case of foliage resistance to late blight, the positions of the majority of the effects, which were located on eleven of the twelve potato linkage groups, have been detected in previous [16] and parallel studies [21]. The absence of Solanum demissum-derived R genes for hypersensitive response to late blight and the co-localisation of QTL for resistance, vigour and earliness suggest that developmental and/or physiological factors play a major role in determining the level of foliage resistance in this population. In contrast with previous findings, a negative correlation was found between foliage and tuber blight resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009601427062
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  • 10
    Electronic Resource
    Electronic Resource
    Linkage between RFLP markers and genes affecting kernel hardness in wheat (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 580-586 
    Details
    ISSN: 1432-2242
    Keywords: Kernel hardness ; Wheat ; RFLP ; QTL ; Puroindoline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A molecular-marker linkage map of wheat (Triticum aestivum L. em. Thell) provides a powerful tool for identifying genomic regions influencing breadmaking quality. A variance analysis for kernel hardness was conducted using 114 recombinant inbred lines (F7) from a cross between a synthetic and a cultivated wheat. The major gene involved in kernel hardness, ha (hard), known to be on chromosome arm 5DS, was found to be closely linked with the locus Xmta9 corresponding to the gene of puroindoline-a. This locus explained around 63% of the phenotypic variability but there was no evidence that puroindoline-a is the product of Ha (soft). Four additional regions located on chromosomes 2A, 2D, 5B, and 6D were shown to have single-factor effects on hardness, while three others situated on chromosomes 5A, 6D and 7A had interaction effects. Positive alleles were contributed by both parents. A three-marker model explains about 75% of the variation for this trait.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417951
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