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  • 1
    Electronic Resource
    Electronic Resource
    Application of Sucrose Synthase from Rice Grains for the Synthesis of Carbohydrates (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 750 (1995), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1995.tb19975.x
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  • 2
    Electronic Resource
    Electronic Resource
    Combined preparative enzymatic synthesis of dTDP-6-deoxy-4-keto-D-glucose from dTDP and sucrose (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 139-145 
    Details
    ISSN: 1573-4986
    Keywords: activated deoxysugars ; preparative enzymatic synthesis ; sucrose synthase ; dehydratase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract dTDP–6–deoxy–4–keto–D–glucose (1), the common intermediate in the biosyntheses of the mainfold deoxysugars, was synthesized on a gram–scale by the combination of sucrose synthase and dTDP–D–glucose 4,6–dehydratase in a fed batch, starting the reaction with dTDP. This process allowed a dTDP conversion with a 100% rate. An easy and efficient three–step purification with anion–exchange chromatography and gel filtration gave 1.1 g of 1 in an overall yield of 73%. This work realizes a first step for an economic access to activated deoxysugars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006912121278
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  • 3
    Electronic Resource
    Electronic Resource
    Recovery and purification of bioproducts: I (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 48 (1995), S. 301-301 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260480402
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  • 4
    Electronic Resource
    Electronic Resource
    Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 45 (1995), S. 205-211 
    Details
    ISSN: 0006-3592
    Keywords: monoclonal antibodies ; fermentation ; fluidized bed adsorption ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: To achive the coarse purification of a monoclonal antibody from whole hybridoma fermentation broth a fluidized bed cation exchange process was used. The procedure consisted of application of the crude sample and washing of the bed in a fluidized mode and elution in a fixed bed mode. A completely clarified eluate was obtained with purification factors between 4 and 8 and a concentration of the desired product (monoclonal antibody) by a factor of more than 3 was achived. Thus, a combination of the three early steps of the downstream process clarification, concentration and coarse purification was possible. Two different materials were tested: a commercially available agarose-based matrix (Stream-line-SP), and a self-derivatized material based on controlled-pore glass (Bioran). Initial experiments were performed to describe the fluidization of the glass material. Comparison with the agarose material showed several differences, the agarose matrix allowing liquid flow closer to plug flow than the glass material. Increased backmixing in the liquid phase was detected when fluidizing the glass adsorbent compared with the agarose-based matrix. Despite this fact, comparison of the two materials with respect to antibody binding and elution demonstrated a similar performance. © 1995 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260450304
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  • 5
    Electronic Resource
    Electronic Resource
    Hydrodynamics and performance in fluidized bed adsorption (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 48 (1995), S. 367-374 
    Details
    ISSN: 0006-3592
    Keywords: adsorption ; fluidization ; hydrodynamics ; proteinpurification ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The performance of fluidized bed adsorption is strongly influenced by the hydrodynamics of the fluidization process. Especially axial mixing in the liquid and solid phase may lead to reduced capacity and resolution. In this article axial mixing in the liquid phase of a classified fluidized bed based on porous glass granules is presented. Axial mixing was analyzed by measurements of residence time distributions in a fluidized bed, showing a reduction of mixing at increased ratio of bed height to diameter as well as at increased linear velocity of the liquid stream. These results were transferred to two real adsorption systems on two different scales: In a bench scale (up to 15 mL of adsorbent) the purification of monoclonal antibodies from hybridoma supernatant was performed with a cation exchanger, in a larger scale (up to 750 mL of matrix) the adsorption of bovine serum albumin (BSA) on the same matrix was investigated. The results showed an increase of capacity at increased bed height-to-diameter ratio; with regard to linear velocity a broad range of only slightly changed capacity was found. A shift from dispersion controlled to diffusion controlled adsorption at intermediate linear velocity was proposed by isolating the effect of pore diffusion from the effect of dispersion. © 1995 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260480409
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  • 6
    Electronic Resource
    Electronic Resource
    Enzyme yields from cells of brewer's yeast disrupted by treatment in a horizontal disintegrator (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 623-634 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Maltase, phosphatase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase activities were determined in the supernatant of brewer's yeast cells from samples taken at various intervals during a 90 min treatment in a Dyno-Mill disintegrator.In an attempt to find optimum operating conditions, the following parameters were varied: speed of rotation of the agitator, flow rate, yeast concentration in the slurry and bead diameter, and the specific rate of extraction was determined for each such variation. Analysis of the data showed that, superimposed upon extraction, there was a progressive but slow loss of total activities. Enzyme release could be expressed as an approximately first-order chemical reaction. The results compared well with those of Follows et al. (Biotechnol. Bioeng.), 13, 549 (1971) and showed that the Dyno-Mill can be operated under proper conditions without significant loss of enzyme activity and with high yields.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260160506
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of electrostatic interactions on partitioning in aqueous polyethylene glycol/dextran biphasic systems: Part I (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 46 (1995), S. 443-451 
    Details
    ISSN: 0006-3592
    Keywords: aqueous two-phase systems ; polyelectrolytes, partition of ; DEAE-dextran ; TMA-dextran ; poly-L-lysine ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: To study the influence of charges on the partition of solutes in aqueous two-phase systems of polyethylene glycol and dextran, partition coefficients of dimethylaminoethyl-dextran, trimethylamino-dextran, and bis (α,ω)-amino-poly(ethylene glycol) were determined as a function of pH (range 2 to 12) and ionic strength. These polymers are derivatives of the phase forming components and carry ionizable groups that are charged or uncharged depending on the pH. Unexpectedly, the largest differences in the partition coefficients were found at high pH, where the modified polymers are uncharged. In addition, the partitioning of low-molecular-weight model compounds, ethylenediamine and iminodiacetic acid, as well as poly-L-lysine and poly(allylamine) was analyzed. A consistent pattern was observed in the partition of polyelectrolytes reflecting the influence of charge, but another property of aqueous phase systems unrelated to charge and changing with pH seems to be superimposed. © 1995 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260460508
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  • 8
    Electronic Resource
    Electronic Resource
    Plasma protein fractionation with advanced membrane adsorbents (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 54 (1997), S. 181-189 
    Details
    ISSN: 0006-3592
    Keywords: membrane adsorption ; human serum albumin ; ion exchange membrane ; plasma fractionation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: High capacity membrane adsorbents have been used as a stationary phase for the preparative chromatographic purification of human serum albumin. A two-step ion exchange fractionation scheme yields albumin with 98% purity from clarified, microfiltrated, and desalted human plasma. Experiments with laboratory and pilot scale membrane modules are compared to literature data obtained with conventional Fast Flow Sepharose in a similar purification protocol. Increased productivity in combination with excellent reproducibility and stability was found using the membrane adsorbents. Scale-up of the process based on standard microfiltration equipment was successful but resulted in reduced capacity and productivity due to deteriorated flow characteristics of the module. This was attributed to the effects of substantial axial dispersion in the pilot scale module. Methods to reduce this limitation were identified. The concept of membrane adsorption chromatography for the fast purification of proteins is illustrated and engineering aspects important for the process design are discussed. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 181-189, 1997.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    26. Methodenentwicklung zur effizienten Optimierung von wäßrigen Zwei-Phasen-Systemen zur Aufarbeitung von Proteinen (1999)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 71 (1999), S. 949-949 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330710930
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  • 10
    Electronic Resource
    Electronic Resource
    Synthesen von dTDP-6-Desoxy-4-ketoglucose und deren Analoga mit nativer und rekombinanter dTDP-Glucose-4,6-dehydrataseDiese Arbeit wurde gefördert von der Deutschen Forschungsgemeinschaft (W.K.), vom Fonds der Chemischen Industrie (W.K.) und von der Studienstiftung des Deutschen Volkes (A.S.). Wir danken Dr. V. Sinnwell und Dr. C. Schulze (Universität Hamburg) für die Aufnahme der NMR- bzw. ESI-Spektren. dTDP = Thymidin-5′-diphosphat. (1995)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 107 (1995), S. 1881-1883 
    Details
    ISSN: 0044-8249
    Keywords: Biotechnologie ; Chromatographie ; Dehydratasen ; Desoxyzucker ; Enzyme ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19951071615
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