ISSN:
1432-203X
Keywords:
Key words Aluminium uptake
;
Brefeldin A
;
Cell culture
;
Nicotiana tabacum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The relationship between cellular growth and aluminium (Al) uptake was examined by applying brefeldin A, a vesicle transport inhibitor, to cultured tobacco (Nicotiana tabacum L. cv. BY-2) cells. Cultured cells almost completely lost the capacity for Al uptake when pre-incubated for 1–3 h in a minimal medium. Pre-incubation also diminished subsequent growth in a culture medium. However, competency for Al uptake (20 μm, pH 4.5) was sustained in a dose-dependent and reversible manner when cells were treated with brefeldin A (10 μm), an inhibitor of Golgi-mediated secretion, prior to and during the incubation in minimal medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050651
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