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  • Barley (Hordeum vulgare L.)  (1)
  • Positron lifetimes  (1)
  • 1995-1999  (1)
  • 1970-1974  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Positron lifetimes in five phases of BEA (1974)
    Springer
    Applied physics 3 (1974), S. 155-158 
    Details
    ISSN: 1432-0630
    Keywords: Positron lifetimes ; Liquid crystals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Positron lifetimes have been measured as a function of temperature in 4-butyloxybenzal-4′-ethylaniline (BEA). BEA has been previously reported to have two liquidcrystalline phases (smectic and nematic) with transition temperatures as follows: solid → smectic, 40.5° C; smectic → nematic, 51.0° C; and nematic → isotropic liquid, 65.5° C. Positron life time spectra were resolved into two components, with the shorter component τ1 remaining approximately constant in all phases, and τ2 exhibiting reversible changes at all of the above transitions. In addition, an irreversible discontinuity in the τ2 lifetime was observed in the vicinity of 28° C, indicating the presence of a new phase (phase X) of BEA. The τ2 andI 2 values obtained for the various phases of BEA are: solid (1.25 nsec, 7.1%), phase X (1.97 nsec, 26.2%), smectic (2.36 nsec, 23.6%), nematic (2.72 nsec, 28.3%), and isotropic liquid (2.69 nsec, 29.8%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00884414
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  • 2
    Electronic Resource
    Electronic Resource
    Fertile transgenic barley generated by direct DNA transfer to protoplasts (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 707-712 
    Details
    ISSN: 1432-2242
    Keywords: Barley (Hordeum vulgare L.) ; Protoplast ; Regeneration ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the generation of transgenic barley plants via PEG-mediated direct DNA uptake to protoplasts. Protoplasts isolated from embryogenic cell suspensions of barley (Hordeum vulgare L. cv ‘Igri’) were PEG-treated in a solution containing a plasmid which contained the neomycin phosphotransferase (NPT II) gene under the control of the rice actin promoter and the nos terminator. Colonies developing from the treated protoplasts were incubated in liquid medium containing the selective antibiotic G418. Surviving calli were subsequently transferred to solid media containing G418, on which embryogenic calli developed. These calli gave rise to albino and green shoots on antibiotic-free regeneration medium. NPT II ELISA revealed that approximately half of the morphogenic calli expressed the foreign gene. In total, 12 plantlets derived from NPT-positive calli survived transfer to soil. Southern hybridization analysis confirmed the stable transformation of these plants. However, the foreign gene seemed to be inactivated in plants from one transgenic line. Most of the transgenic plants set seed, and the foreign gene was transmitted and expressed in their progenies, which was ascertained by Southern hybridization and NPT II ELISA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220947
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