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  • Springer  (5,048)
  • Oxford University Press  (406)
  • 1995-1999  (4,925)
  • 1970-1974  (529)
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  • 1
    ISSN: 1572-879X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The influence of the sulfidation temperature of dehydrated ion exchanged CoNaY on the catalytic activity and structure was studied by thiophene HDS activity measurements, overall sulfur analysis, temperature programmed sulfidation, Xe adsorption measurements in combination with129Xe NMR, EXAFS and ESR. It was shown that up to a sulfidation temperature of 573 K small highly active Co sulfide clusters were formed in the supercages. Sulfidation above 573 K led to decomposition of these Co sulfide particles by a protolysis reaction resulting in the formation of H2Sand a blue colored Co compound having almost no HDS activity.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 83 (1997), S. 252-256 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Confocal laser scanning microscopy and transmission electron microscopy were used to study the inner membrane complex of tachyzoites of Toxoplasma gondii. DiOC6, a lipophilic cationic fluorescent dye used to visualize the endoplasmic reticulum of eukaryotic cells, labeled cytoplasmic structures in a reticulated pattern and the periphery of the nucleus of the host cell. Intracellular and extracellular tachyzoites were stained. Observation of several focal planes showed labeling of the most peripheral region of the protozoan. Reaction product was observed in the outer nuclear membrane, in profiles of the endoplasmic reticulum, and in the inner membrane complex of tachyzoites subjected to the KI-OsO4 technique. Taken together, these observations suggest that the inner membrane complex may represent a specialized region of the endoplasmic reticulum of tachyzoites of T. gondii.
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  • 3
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 23 (1999), S. 682-685 
    ISSN: 1476-5535
    Keywords: Keywords: Trichoderma; xylan; xylanase; characterization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new xylanase (XYL2) was purified from solid-state cultures of Trichoderma harzianum strain C by ultrafiltration and gel filtration. SDS-PAGE of the xylanase showed an apparent homogeneity and molecular weight of 18 kDa. It had the highest activity at pH 5.0 and 45°C and was stable at 50°C and pH 5.0 up to 4 h xylanase. XYL2 had a low K m with insoluble oat spelt xylan as substrate. Compared to the amino acid composition of xylanases from Trichoderma spp, xylanase XYL2 presented a high content of glutamate/glutamine, phenylalanine and cysteine, and a low content of serine. Xylanase XYL2 improved the delignification and selectivity of unbleached hardwood kraft pulp.
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  • 5
    ISSN: 1570-7458
    Keywords: Anthocoridae ; Orius laevigatus ; Orius albidipennis ; reproduction ; Ephestia kuehniella ; pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Reproduction and longevity of the anthocorid predators Orius laevigatus (Fieber) and Orius albidipennis (Reuter) (Heteroptera: Anthocoridae) were studied under controlled conditions in the laboratory. Three different diets were tested: eggs of the Mediterranean flour moth. Ephestia kuehniella Zeller, E. kuehniella eggs plus pollen, and pollen only. Small rooted plants of Spanish pepper (Capsicum annuum L. cv. ‘Creta’, long red) served as oviposition substrate and moisture source. In all treatments, total fecundity, oviposition period and female longevity of O. laevigatus were greater than that of O. albidipennis. When a diet of E. kuehniella eggs was supplemented with pollen, total fecundity of O. albidipennis was increased by about 40%. Female O. albidipennis fed only pollen laid five times less eggs than those fed flour moth eggs and pollen; also, longevity of females was shorter when only pollen was offered as food. Fecundity and longevity of O. laevigatus were not influenced when supplementing a diet of E. kuehniella eggs with pollen. Females of O. laevigatus receiving only pollen reduced egg production by about 60% but had a similar longevity as those receiving a diet including flour moth eggs. In either species, preoviposition period and egg hatch were not affected by diet. The ability of both anthocorids to use pollen as an alternative or additional food is discussed in relation to their practical use in integrated control programmes.
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  • 6
    ISSN: 1434-601X
    Keywords: PACS:23.20.Lv Gamma transitions and level energies – 25.70.Jj Fusion and fusion-fission reactions – 27.70.+q 150 ≤ A ≤ 189
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. The 178Pt and 181Au nuclei have been studied with the (230 MeV) 46Ti +142Nd fusion-evaporation reaction; γ-rays, evaporated particles and recoiling nuclei have been detected by the γ-array GASP + the Si ball ISIS + the recoil mass spectrometer RMS. The newly observed structures in 178Pt and 181Au are most probably built on the proton and quasi-proton h9/2 and i13/2 configurations.
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  • 7
    ISSN: 1572-879X
    Keywords: positron emission profiling (PEP) ; in situ study ; transient kinetics ; hexane hydroisomerization ; acidic zeolite catalysts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The ability of positron emission profiling (PEP) to measure concentration profiles of molecules labelled with positron-emitting nuclei, such as 11C, 13N, and 15O, inside chemical reactors has been demonstrated for the system n-hexane–Pt/H-zeolites under conditions typical of the hydroisomerization reaction. Data obtained in the absence of reaction were first measured and used to model mass transport processes in these biporous, packed-bed reactors. Images obtained under conditions where injected, labelled pulses underwent reaction revealed that the products did not exit the reactor and thus demonstrated the need for in situ measurement. Such experiments should provide a valuable new tool in the study of transient, initial phenomena so often of importance in heterogeneous catalysis, such as “preconditioning” and deactivation.
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  • 8
    ISSN: 1432-0827
    Keywords: Key words: Osteoclast — Osteoblast — Cell density — Interleukin-6 — Macrophage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Osteoclast formation from the hemopoietic stem cell line FDCP-mix C2GM was shown to be strongly dependent on osteoblast density. In cocultures of C2GM cells with fetal mouse osteoblasts seeded at high density (i.e., 2.5 × 104 cells/cm2), we found a significantly lower osteoclast formation compared with cocultures with osteoblasts seeded at low density (i.e., 1 × 104 cells/cm2). The differentiation state of osteoblasts in high-density cultures resembled more than that of osteoblasts in low-density cultures, the differentiation state of mature osteoblasts, since the cells in the former cultures showed higher alkaline phosphatase (APase) activity than the cells in the latter cultures, and nodules were formed in high-density cultures but not in low-density cultures. Endogenous interleukin-6 (IL-6) production was found to be significantly lower in high-density cultures, which may partly explain the impaired osteoclast formation in high-density cocultures. Addition of IL-6 to the high-density cocultures indeed restored osteoclast formation. There appeared to be no overt difference in IL-6 receptor mRNA expression between high-density and low-density cultures. In conclusion, this paper suggests that mature, highly differentiated osteoblasts are not directly involved in osteoclastogenesis. In contrast, osteoblast-like cells lacking mature osteoblast markers induce osteoclast formation. Whether these low-density osteoblast-like cells represent an immature differentiation state or the lining cell phenotype is unclear.
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  • 9
    ISSN: 1432-1041
    Keywords: Key words Folic acid ; Neural tube defects ; Public health education ; Awareness ; Behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objective: In November 1993, Dutch health authorities advised that women planning a pregnancy should take folic acid in the periconceptional period to reduce the risk of fetal neural tube defects. In the autumn of 1995 a national campaign was organized to inform women and health care professionals in a systematic way. Methods: We assessed the awareness and behaviour of women at their first or second antenatal visit in two surveys in the spring of 1994 and the autumn of 1995, in order to evaluate the impact of non-systematic information during that period, and to collect baseline data to evaluate the effect of the national folic acid campaign. The two surveys were carried out in the north and the west of The Netherlands. Results: The proportion of women who had heard of folic acid increased from 28% to 78%. The proportion that used folic acid during any period in pregnancy increased from 7.8% to 26%. The proportion that took folic acid tablets during the whole of the recommended period increased from 0.8 to 4.4%. In the group of women who did not take folic acid, the proportion who did “not like to use anything during pregnancy” decreased, as did the proportion who did “not think it is useful”. Conclusion: In The Netherlands, non-systematic information about periconceptional folic acid use has already led to significant changes in awareness and behaviour before the start of the national folic acid campaign.
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  • 10
    ISSN: 1617-4623
    Keywords: Key words Antibody production ; DNA methylation ; Gene silencing ; Instability ; Transgene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The stability of antibody and Fab expression was assessed in five different homozygous transgenic Arabidopsis lines. Each of these lines showed silencing of the transgenes that encode the antibody polypeptides, leading to instability of antibody production. However, each line had a different and specific instability profile. The characteristic variation in the level of antibody accumulation in each line as a function of developmental stage indicated that the T-DNA integration pattern played a role in triggering silencing, and also that the history and the integration position of simple transgene loci can influence the susceptibility to epigenetic silencing. In different lines with low antibody accumulation levels, methylation was found either in the promoter alone, in both the promoter and the transcribed region, in the transcribed region only, or in the transcribed region and downstream sequences. In conclusion, our data suggest that epigenetic effects result in different transgene expression profiles in each of the five Arabidopsis lines analyzed.
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