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  • 1
    Electronic Resource
    Electronic Resource
    Die mechanische Kopplung im monolithischen Filter (1972)
    Springer
    Zeitschrift für angewandte Mathematik und Physik 23 (1972), S. 1023-1023 
    Details
    ISSN: 1420-9039
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01596242
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  • 2
    Electronic Resource
    Electronic Resource
    Monolithische filter (1972)
    Springer
    Zeitschrift für angewandte Mathematik und Physik 23 (1972), S. 167-167 
    Details
    ISSN: 1420-9039
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01593225
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  • 3
    Electronic Resource
    Electronic Resource
    Heme synthetase in thalassemia (1972)
    Springer
    Cellular and molecular life sciences 28 (1972), S. 193-193 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Hämsynthetase (HS)-Aktivität zirkulierender Retikulozyten und Erythrozyten von Normalspendern und Patienten mit Thalassämie wurde in vitro bestimmt. HS war normal oder erhöht bei Thalassämie. Jugendliche, über das Stadium des Retikulozyten hinaus gereifte Erythrozyten enthalten wahrscheinlich noch einen signifikanten Anteil der im peripheren Blut messbaren HS-Aktivität.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01935753
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular deficiencies of human blood coagulation (1972)
    Springer
    Cellular and molecular life sciences 28 (1972), S. 1-7 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Blut gerinnt, wenn das Gerinnungsferment Thrombin aktiviert wird und Fibrinogen in Fibrin umwandelt. Störungen der Thrombin- oder Fibrinbildung können Bluterkrankheiten verursachen. Die Ursache angeborener Bluterkrankheiten liegt entweder in der quantitativen Verminderung oder in qualitativen Strukturanomalien eines Gerinnungsfaktors. Immunologische Methoden im Verein mit einer beginnenden, direkten Strukturabklärung zeigten für das Fibrinogen überzeugend, dass beide Möglichkeiten vorkommen. Unterschiedliche Funktionsausfälle von Fibrinogen lassen vermuten, dass verschiedenartigen Strukturanomalien für Störungen der Substrateigenschaften gegenüber Thrombin, der Polymerisierung oder der Fibrinstabilisierung verantwortlich sind. Ähnliche Untersuchungen an den Faktoren II, VII, VIII, IX, X und XIII zeigen, dass wahrscheinlich die Mehrzahl der bekannten Bluterkrankheiten durch Strukturanomalien verursacht werden. Die eingehende Untersuchung solcher Strukturdefekte wird zukünftig um so interessanter und bedeutungsvoller werden, je besser die Struktur und Funktion der normalen Gerinnungsfaktoren bekannt sind.
    Notes: Summary Blood coagulation reflects, the complex biological system resulting in the formation of thrombin and the subsequent conversion of fibrinogen into fibrin. Disorders of either thrombin or fibrin formation may cause bleeding states. Congential hemorrhagic disorders are characterized by either reduction of a coagulation factor or impairment of its functional capacity by structural aberrations. Immunological studies and direct molecular evaluation of fibrinogen demonstrate that both types of abnormalities occur. Functional evaluation of abnormal fibrinogens suggests that many different structural variants exist and variably influence interaction with thrombin, fibrin polymerization and fibrin ‘stabilization’. Similar studies performed on factors II, VII, VIII, IX, X and XIII indicate that qualitative abnormalities are more frequent than ‘true’ deficiency states. Such molecular abnormalities will become increasingly interesting and important as the biochemistry of normal coagulation progresses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01928228
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  • 5
    Electronic Resource
    Electronic Resource
    Growth and alkaloid contents in leaves of Tabernaemontana pachysiphon Stapf (Apocynaceae) as influenced by light intensity, water and nutrient supply (1996)
    Springer
    Oecologia 107 (1996), S. 160-169 
    Details
    ISSN: 1432-1939
    Keywords: Monoterpene indole alkaloids ; Tropical trees ; Abiotic factors ; Growth ; C/N balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of Tabernaemontana pachysiphon (Apocynaceae) plants and the alkaloid content of leaves were investigated in the greenhouse at three levels of nutrient supply under two contrasting water and light regimes. We determined height increment, above-ground biomass production, leaf size, specific leaf weight and the content of the alkaloids apparicine, A2, isovoacangine, tubotaiwine and tubotaiwine-N-oxide. The effects of major controlling factors such as light, water and nutrient supply could be directly correlated with growth and were largely independent of each other. In contrast, leaf-alkaloid contents were influenced by interdependencies among the main factors and individually affected in a synergistic or antagonistic manner which deviated from the effects on growth. The following general trends could be identified with respect to the quantitatively predominant alkaloids apparicine, tubotaiwine and isovoacangine. Increasing nutrient supply had a positive effect on both growth and alkaloid content. Drought increased alkaloid content, but retarded growth. High light intensity lowered alkaloid content but promoted growth. We investigated the relationship between primary production and the production of secondary metabolites with respect to relative and total alkaloid content as well as in relation to the leaves' nitrogen status. Our results showed that under conditions of low nutrient supply, higher proportions of leaf nitrogen were allocated to alkaloids than at moderate or high nutrient supply. Under conditions of drought and low light, all plants allocated almost equal proportions of leaf nitrogen to alkaloids, regardless of fertiliser. Total alkaloid content per plant, however, increased with fertilisation. With respect to the N-allocation strategy, we found no indication of a trade-off between primary production and the production of secondary metabolites in this species. Rather, our results are in accordance with the carbon nutrient balance hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327899
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  • 6
    Electronic Resource
    Electronic Resource
    Frost hardening and photosynthetic performance of Scots pine (Pinus sylvestris L.). II. Seasonal changes in the fluidity of thylakoid membranes (1998)
    Springer
    Planta 204 (1998), S. 201-206 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Electron paramagnetic resonance ; Frost hardening ; Membrane fluidity ; Photoperiod ; Scots pine ; Thylakoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The fluidity of chloroplast thylakoid membranes of frost-tolerant and frost-sensitive needles of␣three- to four-year-old Scots pine (Pinus sylvestris L.) trees, of liposomes produced from the lipids of the thylakoids of these needles, and of liposomes containing varying amounts of light-harvesting complex (LHC) II protein was investigated by means of electron paramagnetic resonance (EPR) measurements using spin-labelled fatty acids as probes. Broadening of the EPR-resonance signals of 16-doxyl stearic acid in chloroplast membranes of frost-sensitive needles and changes in the amplitudes of the peaks were observed upon a decrease in temperature from +30 °C to −10 °C, indicating a drastic loss in rotational mobility. The lipid molecules of the thylakoid membranes of frost-tolerant needles exhibited greater mobility. Moderate frost resistance could be induced in Scots pine needles by short-day treatment (Vogg et al., 1997, Planta, this issue), and growth of the trees under short-day illumination (9 h) resulted in a higher mobility of the chloroplast membrane lipids than did growth under long-day conditions (16 h). The EPR spectrum of thylakoids from frost-tolerant needles at −10 °C was typical of a spin label in highly fluid surroundings. However, an additional peak in the low-field range appeared in the subzero temperature range for the chloroplast membranes of frost-sensitive needles, which represents spin-label molecules in a motionally restricted surrounding. The EPR spectra of thylakoids and of liposomes of thylakoid lipids from frost-hardy needles were identical at +30 °C and −10 °C. The corresponding spectra from frost-sensitive plants revealed an additional peak for the thylakoids, but not for the pure liposomes. Hence, the domains with restricted mobility could be attributed to protein-lipid interactions in the membranes. Broadening of the spectrum and the appearance of an additional peak was observed with liposomes of pure distearoyl phosphatidyl glycerol modified to contain increasing amounts of LHC II. These results are discussed with respect to a loss of chlorophyll and chlorophyll-binding proteins in thylakoids of Scots pine needles under winter conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050247
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  • 7
    Electronic Resource
    Electronic Resource
    Frost hardening and photosynthetic performance of Scots pine (Pinus sylvestris L.) needles. I. Seasonal changes in the photosynthetic apparatus and its function (1998)
    Springer
    Planta 204 (1998), S. 193-200 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Chlorophyll ; Chlorophyll-binding protein ; Frost hardening ; Photosynthesis ; Pinus (frost hardening)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Photosynthetic CO2 uptake, the photochemical efficiency of photosystem II, the contents of chlorophyll and chlorophyll-binding proteins, and the degree of frost hardiness were determined in three-year-old Scots pine (Pinus sylvestris L.) trees growing in the open air but under controlled daylength. The following conditions were compared: 9-h light period (short day), 16-h light period (long day), and natural daylength. Irrespective of induction by short-day photoperiods or by subfreezing temperatures, frost hardening of the trees was accompanied by a long-lasting pronounced decrease in the photosynthetic rates of one-year-old needles. Under moderate winter conditions, trees adapted to a long-day photoperiod, assimilated CO2 with higher rates than the short-day-treated trees. In the absence of strong frost, photochemical efficiency was lower under short-day conditions than under a long-day photoperiod. Under the impact of strong frost, photochemical efficiency was strongly inhibited in both sets of plants. The reduction in photosynthetic performance during winter was accompanied by a pronounced decrease in the content of chlorophyll and of several chlorophyll-binding proteins [light-harvesting complex (LHC)IIb, LHC Ib, and a chlorophyll-binding protein with MW 43 kDa (CP 43)]. This observed seasonal decrease in photosynthetic pigments and in pigment-binding proteins was irrespective of the degree of frost hardiness and was apparantly under the control of the length of the daily photoperiod. Under a constant 9-h daily photoperiod the chlorophyll content of the needles was considerably lower than under long-day conditions. Transfer of the trees from short-day to long-day conditions resulted in a significantly increased chlorophyll content, whereas the chlorophyll content decreased when trees were transferred from a long-day to a short-day photoperiod. The observed changes in photosynthetic pigments and pigment-binding proteins in Scots pine needles are interpreted as a reduction in the number of photosynthetic units induced by shortening of the daily light period during autumn. This results in a reduction in the absorbing capacity during the frost-hardened state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050246
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  • 8
    Electronic Resource
    Electronic Resource
    Specificity of σ-dependent binding of RNA polymerase to DNA (1972)
    Springer
    Molecular genetics and genomics 118 (1972), S. 199-207 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although a large number of E. coli RNA polymerase molecules can bind to phage T3 DNA, not more than three remain bound per DNA template after addition of poly inosinic acid (poly I) which has a high affinity for the enzyme. These stable complexes are able to initiate RNA chains without lag as the enzyme is resistant against rifampicin if substrate is added simultaneously with the drug. Poly I resistant complexes decay very rapidly in the cold (Fig. 2) and are not formed in the absence of the polymerase σ factor (Table 2). The data provide additional support for the idea that the σ factor effects the binding of the enzymes to specific sites on the DNA template.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333456
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  • 9
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    Molecular deficiencies of human blood coagulation (1972)
    Springer
    Details
    Publication Date: 1972-01-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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  • 10
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    Heme synthetase in thalassemia (1972)
    Springer
    Details
    Publication Date: 1972-02-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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