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  • Chloroplasts  (1)
  • Data reduction  (1)
  • Springer  (2)
  • 1995-1999  (2)
  • 1970-1974
  • 1965-1969
  • 1945-1949
Collection
Keywords
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  • Springer  (2)
Years
  • 1995-1999  (2)
  • 1970-1974
  • 1965-1969
  • 1945-1949
Year
  • 1
    Electronic Resource
    Electronic Resource
    Comparison of the expression of a plastidic chaperonin 60 in different plant tissues and under photosynthetic and non-photosynthetic conditions (1996)
    Springer
    Planta 200 (1996), S. 326-334 
    Details
    ISSN: 1432-2048
    Keywords: Chaperone ; Chloroplasts ; Chromoplasts ; Heat-shock protein ; Secale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A partial cDNA which codes for the β-subunit of a plastidic chaperonin 60 (cpn60-β) from rye (Secale cereale L.) leaves was identified and sequenced, except for 46 amino acids of the N-terminus of the mature protein and the transit sequence. This is the first cpn60-β sequence determined for a monocotyledonous plant. Specific antibodies against cpn60-β were affinity-purified from an antiserum raised against the total soluble protein fraction of ribosome-deficient plastids. The localization of cpn60-β in chloroplasts or non-green plastids was confirmed by immunodetection in Percoll gradient-purified organelles. The expression and occurrence of cpn60-β was analysed by immunoblotting with the specific antibodies and Northern hybridization. The cpn60-β protein was constitutively expressed in various green and non-green tissues. It was evenly distributed along the major part of a rye leaf, while highest transcript levels occurred in the youngest and oldest leaf sections. The expression of the cpn60-β protein was not enhanced by a heat-shock treatment at 42 °C. The cpn60-β transcript and protein were more strongly expressed in various non-green, for instance etiolated, 70S-ribosome-deficient 32 °C-grown, or herbicide-bleached tissues, than in green leaves of rye. A rapid increase in the cpn60-β transcript level was also observed when green leaves were transferred from light to darkness while the protein level was not affected. The dark-induced increase in the cpn60-β transcript was totally suppressed in the presence of 2% sucrose. Inhibitor treatments suggested that the change in cpn60-β transcript level was not related to changes of the ATP supply of the tissue. While the large subunit of the photosynthetic protein ribulose-1,5-bisphosphate carboxylase was largely degraded during ripening of tomato fruits, high levels of cpn60-β were detected in tomato chromoplasts and in the yellow flower petals of Narcissus. Low levels of cpn60-β were detected in root tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200300
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Approximate solution of normal equations by eigenvalue decomposition (1999)
    Springer
    Journal of geodesy 73 (1999), S. 109-117 
    Details
    ISSN: 1432-1394
    Keywords: Key words. Multivariate Gauss ; Markoff model ; Eigenvalue decomposition ; Principal-component analysis ; Data reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying
    Notes: Abstract. Geodetic adjustment problems frequently require the solution of large systems of linear equations. An approximation method is presented based on the decomposition of the estimated covariance matrix of the observation matrix, calculated in a pre-processing step, into a system of eigenvalues and eigenvectors. Neglecting the non-dominant eigenvalues and the assigned eigenvectors, the matrix of the residuals is approximated applying the synthesis formula of principal-component analysis. Although the number of observation vectors in the multivariate Gauss–Markoff model is drastically reduced, all unknown parameters are estimated approximately. The described method is tested using a numerical example of satellite altimetry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001900050225
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    Paper (German National Licenses)
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