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  • 79.40  (1)
  • Epoxide hydrolase  (1)
  • Polymer and Materials Science
  • Springer  (2)
  • 1995-1999  (1)
  • 1975-1979  (1)
  • 1
    ISSN: 1432-2048
    Keywords: Epoxide hydrolase ; Epoxy fatty acid ; Glyoxysome kw]Ricinus ; Seed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epoxide hydrolase (EC 3.3.2.3) activity was measured with [1-14C]cis-9,10-epoxystearic acid as the substrate. Homogenates were prepared from the endosperm tissue of germinating seeds of castor bean (Ricinus communis L. zanzibariensis). The activity of fatty-acid epoxide hydrolase was characterized with respect to dependence on time, amount of protein, pH and temperature. Analyses of enzyme distribution in endosperm, cotyledons, root and hypocotyl showed the highest total activity in the endosperm, less in the cotyledons and low activity in the root and hypocotyl. The specific activity was similar for cotyledons and endosperm. Analysis of the temporal expression of the enzyme in the endosperm during germination revealed high activity already in the imbibed seed. Activity was maximal between days four to six and then decreased at the end of one week. Subcellular fractionation of endosperm revealed a dual distribution of activity between the glyoxysomal and the cytosolic fractions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 10 (1976), S. 265-268 
    ISSN: 1432-0630
    Keywords: 79.40 ; 73
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The field emission current from thin palladium films deposited on insulating tips in UHV was measured as a function of film thickness at relatively low field strengths. The observed periodic oscillations of the field emission current can be interpreted as the quantum size effect.
    Type of Medium: Electronic Resource
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