ISSN:
1615-6110
Keywords:
Asteraceae
;
Crepis foetida
;
Subspecies
;
idiograms
;
haematoxylin staining
;
Giemsa C-banding
;
fluorochrome banding
;
Ag-NOR staining
;
genome size
;
Feulgen densitometry
;
propidium iodide flow cytometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Ten populations ofCrepis foetida from Bulgaria belonging to the three subspeciesfoetida, rhoeadifolia, andcommutata were analyzed karyologically using haematoxylin staining, Giemsa C-banding, fluorochrome banding, Ag-NOR staining, Feulgen cytophotometry (scanning densitometry and video-based image analysis), and propidium iodide flow cytometry. The quantitatively-evaluated karyotype structure was similar among all populations, with minor variation in a few intercalary sites only and in the amount of NOR-associated heterochromatin (satellites). In contrast to the karyotypic constancy the genome size ofC. foetida subsp.commutata was about 10% lower than those of the other two subspecies, which had similar genome sizes. The genome size measurements using three different methods resulted in highly correlated data. The genome size difference adds some weight to previous taxonomic opinions treatingC. foetida subsp.commutata at species level, asC. commutata.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00984369
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