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  • 1
    Electronic Resource
    Electronic Resource
    Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system (1997)
    Springer
    Histochemistry and cell biology 108 (1997), S. 95-103 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  PACE4 is a mammalian Kexin family protease that is involved in the maturation of precursor proteins. Four PACE4 isoforms have been identified. We identified a novel PACE4 isoform, PACE4E, from a human cerebellum cDNA library, which possesses a hydrophobic cluster in its C-terminus participating in membrane association. The size of PACE4E mRNA from adult rat brain was estimated by Northern blotting to be 4.4 kb. In situ hybridization histochemistry revealed that the highest level of PACE4E mRNA was expressed in the mitral cells of the adult rat olfactory bulb (OB). The OB is a unique sensory organ in that it has a lifelong regenerating capacity and it affects brain development. We further analyzed the expression of PACE4E mRNA in the developing olfactory system. On day 13.5 of gestation, PACE4E mRNA was expressed at high levels in the neuroepithelium of the forebrain vesicle (FV), olfactory epithelium, and cells in the fiber bundles projecting to the FV. As development proceeded, PACE4E mRNA was expressed in developing mitral cells but decreased in the olfactory epithelium. In the newborn, its expression was confined to the mitral cells in both the main and accessory OB and in some periglomerular cells, as shown in adult rats. The spatio-temporal expression of PACE4E suggests that it plays a role in the establishment and maintenance of the olfactory receptor system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050150
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  • 2
    Electronic Resource
    Electronic Resource
    Grain weevil,Sitophilus granarius (L.): Antennal and behavioral responses to male-produced volatiles (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 1639-1654 
    Details
    ISSN: 1573-1561
    Keywords: Sitophilus granarius ; grain weevil ; Coleoptera ; aggregation pheromone ; electroantennogram ; coupled GC-EAG ; behavioral bioassay ; circular dichroism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Coupled GC-EAG techniques have been applied to the study of volatiles from the grain weevil,Sitophilus granarius. for the first time. The size of EAG response was independent of the sex of the responding insect but was consistently larger to extracts of males than those of females. This difference was reflected in a behavioral preference for the male extracts by mated adults of both sexes tested together and virgin adults of both sexes tested separately. The GC-EAG results provide evidence for two materials that are released specifically by the males. Using circular dichroism. one has been found to be identical stereochemically with the (2S,3R)-sitophilate reported by others as the aggregation pheromone in a different strain. This enhances the prospects for the development of a single pheromone lure that would be generally applicable whatever the origin of the strain. The small amount of sitophilate found in the males suggests that it is not stored in large amounts. The other material, present in such a small amount that it has yet to be fully characterized, elicits a higher antennal activity than sitophilate and may have a significant role to play in enhancing the trap catch of this economically important pest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02272404
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  • 3
    Electronic Resource
    Electronic Resource
    Perception byTrogoderma species of chirality and methyl branching at a site far removed from a functional group in a pheromone component (1980)
    Springer
    Journal of chemical ecology 6 (1980), S. 911-917 
    Details
    ISSN: 1573-1561
    Keywords: Trogoderma species ; trogodermal ; enantiomers ; chiral center ; geometric isomers ; methyl branching ; (Z)- or (E)-14-methyl-8-hexadecenal ; dermestid beetles ; Coleoptera ; Dermestidae ; pheromone ; chemoreception ; olfaction ; (Z)-hexadecenal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Responses to enantiomers of (Z)- and (E)-trogodermal (14-methyl-8-hexadecenal) suggest that fourTrogoderma species utilize the (R)-(−) configuration at C-14. Removal of the C-14 methyl branch decreased the response. These results demonstrate the high specificity associated with the configuration at a chiral center, or the methyl branch, distant in terms of numbers of bonds from a functional group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00990475
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  • 4
    Electronic Resource
    Electronic Resource
    Spined citrus bugs,Biprorulus bibax breddin (Hemiptera: Pentatomidae), do not discriminate between enantiomers in their aggregation pheromone (1995)
    Springer
    Journal of chemical ecology 21 (1995), S. 403-406 
    Details
    ISSN: 1573-1561
    Keywords: Biprorulus bibax ; Hemiptera ; Pentatomidae ; aggregation pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Female spined citrus bugs,Biprorulus bibax, in outdoor flight cage tests did not discriminate between synthetic aggregation pheromone blends containing either the racemate, natural (3R, 4S)-(−) or unnatural (4S, 3S)-(+) enantiomers of the major hemiacetal component (3R, 4S, 1′E)-3,4-bis(1′-butenyl)tetrahydro-2-furanol. Two to three times as many bugs were attracted to pheromone-baited sites compared to unbaited sites. The racemate of the hemiacetal is easier and less costly to synthesize than the enantiomers and will be used in development of the pheromone as a management tool for this pest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02036737
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  • 5
    Electronic Resource
    Electronic Resource
    Chirality of israeli pine bast scale,Matsucoccus josephi (homoptera: Matsucoccidae) sex pheromone (1995)
    Springer
    Journal of chemical ecology 21 (1995), S. 849-858 
    Details
    ISSN: 1573-1561
    Keywords: Matsucoccus josephi ; Coccoidae ; Homoptera ; Matsucoccidae ; pine bast scales ; sex pheromone ; absolute configuration ; (2E,5R,6E,8E)-5,7-dimethyl-2,6,8-decatrien-4-one
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The absolute configuration of the sex pheromone of the Israeli pine bast scale,Matsucoccus josephi, was determined as (2E,5R,6E,8E)-5,7-dimethyl-2,6,8-decatrien-4-one, designated here asR-E with 10% (2E,5S,6E,8E)-5,7-dimethyl-2,6,8-decatrien-4-one, designated asS-E. The chirality of the quantitatively minorZ isomer was (2E,5R,6Z,8E)-5,7-dimethyl-2,6,8-decatrien-4-one (R-Z). Chiral assignments were made by comparative gas chromatographic (GC) analysis of naturalM. josephi pheromone with stereoselectively synthesized stereoisomers on a chiral Cyclodex-B column, which separated the enantiomers with baseline resolution. In gas chromatographic-electroantennographic detection (GC-EAD) analysis of the racemicZ andE isomers, the latter elicited the stronger antennal response by maleM. josephi. In GC-EAD of all four stereoisomers, employing the chiral column,R-E was the most active stereoisomer. In field testsR-E attracted 10 times more males ofM. josephi than didS-E. The racemicE/Z pheromone mixture, containing all four stereoisomers in approximately equal amounts, attracted as many maleM. josephi as did an equivalent amount ofR-E, indicating that the other stereoisomers are not inhibitory. The same keto-diene moiety with the same chiral center and configuration in all three known Matsucoccidae sex pheromones implies a common biosynthetic pathway and phylogenetic relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02033465
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  • 6
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    Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system (1997)
    Springer
    Details
    Publication Date: 1997-08-04
    Print ISSN: 0018-2222
    Electronic ISSN: 1432-119X
    Topics: Biology , Medicine
    Published by Springer
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