ISSN:
1432-203X
Keywords:
Key words Allium sativum
;
Benzyladenine
;
Picloram
;
Thidiazuron
;
2
;
4-D
;
2iP
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 μM 2,4-D and maintained on the same basal medium with 4.7 μM picloram+0.49 μM 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 μM picloram+13.3 μM BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050706
Permalink
