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  • Blackwell Publishing Ltd  (121)
  • 1995-1999  (60)
  • 1980-1984  (47)
  • 1950-1954  (12)
  • 1940-1944  (2)
  • 1920-1924
  • 1
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Microsporidia are obligate intracellular protozoan parasites that can cause opportunistic infections in AIDS patients. Species from five genera of microsporidia are presently known to infect man. One species, Septata intestinalis originally was detected in stool specimens of individuals with chronic diarrhea and subsequently was found to disseminate to the kidneys, lungs, and nasal sinuses. This organism has since been reclassified as Encephalitozoon and in this study, we report the culture of Encephalitozoon intestinalis from a bronchoalveolar lavage specimen and a nasal mucus aspirate of two AIDS patients living in the USA. The bronchoalveolar and nasal microsporidian isolates grew in several continuous cell lines including RK-13, MDCK, HT-29, Caco-2, Vero, and 1047. Transmission electron microscopy of the clinical and cell culture specimens revealed that the new isolates appeared to be E. intestinalis based on morphology and growth of organisms in septated membrane-bound parasitophorous vacuoles. The new E. intestinalis isolates were characterized and compared with the first isolated E. intestinalis that was cultured from stool to confirm their identity and to determine if there existed any minor differences, as seen in the closely related Encephalitozoon cuniculi strains. By the methods of sodium dodecyl sulfate-polyacrylamide gel electrophoresis staining for proteins and carbohydrates, Western blot immunodetection, and polymerase chain reaction-based methods with restriction endonuclease digestion, double-stranded DNA heteroduplex mobility shift analysis, and DNA sequencing of the ribosomal DNA intergenic spacer region, the new isolates were identical to each other and to the reference isolate of E. intestinalis. In addition, with any of these methods, the E. intestinalis organisms could be distinguished from the three E. cuniculi strains, Encephalitozoon hellem, and Vittaforma corneae, which is important for diagnostics, therapeutic strategies, and epidemiology.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 43 (1996), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 42 (1995), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Phylogenetic analysis of the small subunit ribosomal DNA of a broad range of representative microsporidia including five species from humans (Enterocytozoon bieneusi, Nosema corneum, Septata intestinalis, Encephalitozoon hellem and Encephalitozoon cuniculi), reveals that human microsporidia are polyphyletic in origin. Septata intestinalis and E. hellem are very similar to the mammalian parasite E. cuniculi. Based on the results of our phylogenetic analysis, we suggest that S. intestinalis be designated Encephalitozoon intestinalis. Furthermore, analysis of our data indicates that N. corneum is much more closely related to the insect parasite Endoreticulatus schubergi than it is to other Nosema species. This finding is supported by recent studies which have shown a similarity between E. schubergi and N. corneum based on the origin and development of the parasitophorous vacuole. Thus these opportunistic microsporidian parasites can originate from hosts closely or distantly related to humans. Finally, the phylogeny based on small subunit ribosomal DNA sequences is highly inconsistent with traditional classifications based on morphological characters. Many of the important morphological characters (diplokaryon, sporophorous vesicle, and meiosis) appear to have multiple origins.
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  • 4
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Sequences of the small subunit rRNA genes of Amblyospora californica and an Amblyospora sp. from Culex salinarius were determined. These sequences were compared phylogenetically with 16 other microsporidia. The results suggest Amblyospora forms a sister taxon to the rest of the microsporidia examined. The basal position of Amblyospora is discussed with respect to the evolution of microsporidian life cycles. These sequences represent the longest microsporidian small subunit rRNA genes sequenced to date, 1,359 and 1,358 bp, respectively. Structural features and GC content (49% for both) are comparable to those of other microsporidia which have been sequenced.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 14 (1998), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Soil cores from river marginal wetlands in the UK, France and Ireland, sampled and contained within PVC piping, were flooded with nitrate-N enriched water. Half of the cores were sterilized prior to flooding to destroy the denitrifying bacteria. The change in nitrate-N concentration in the floodwater was measured over time. It is argued that the observed nitrate depletion rates (from 0.4 to 2.3 kg/ha per day) may be identified With microbially-mediated denitrification. The results show the method to be a simple and direct procedure for the assessment of spatial variation in nitrate-sink capacity. The depth of the denitrifying layer at the soil-water interface was confirmed to be of the order of a few mm.
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  • 6
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two experiments were carried out to determine whether the differences in intake and feeding value previously shown between two grass cultivars when offered to ruminants as chopped artificially dehydrated (dried) material could also be demonstrated when the grasses were offered in other forms. Two cultivars of tetraploid Italian ryegrass (Lolium multiflorum), Sabalan and Tetila, were established in the same field in 1975. In 1976 they were grazed and conserved (two cuts of primary growth) as dried material or as silage.The composition of the herbage selected at pasture and conserved showed higher concentrations of normal detergent fibre and acid detergent fibre in the dry matter for Tetila than for Sabalan, but differences between cultivars in digestibility were small. In both grazing and winter feeding trials differences in voluntary intake between the two cultivars were not significant, but at pasture young beef cattle spent less time grazing and tended to spend more time ruminating on Tetila than Sabalan. There was no measure of animal performance at pasture but liveweight gain was 15% higher for Sabalan than Tetila when both were offered to young beef cattle as the sole feed of dried grass or of silage. The voluntary intakes of the three forms of feed were very similar, which in part reflected a similarity in digestibility. However, gains were lower for calves given silage than those given dried grass. This may have been due to a lower efficiency in the utilization of the nitrogeneous components of silage for tissue growth than those of dried grass.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 51 (1996), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three small plot experiments were conducted to investigate the effects of species of grass and forbs, defoliation regime, inclusion of white clover and forb blend on the herbage dry matter (DM) yield, botanical composition and mineral content of swards managed with zero fertilizer inputs. The results of all three experiments were characterized by decline in herbage production and large variations in treatment effects over the harvest period.When sown singly with a standard grass mix the species that competed well with grasses and produced annual forb herbage yields greater than 20 t DM ha−1 were black knapweed, oxeye daisy, ribwort plantain, burnet, birdsfoot trefoil, chicory, kidney vetch, red clover and white clover. When sown singly with a standard forb mix, grass species significantly affected the annual yield of total (P〈005). grass (P〈001) and forb (P〈0.001) herbage. The species that most surpressed the yield of forbs were common bent, Yorkshire fog and perennial ryegrass. Those that allowed for the highest yield of forbs were rough meadow grass, sweet vernal grass and crested dogstail. Averaged over the three harvest years, defoliation regime did not significantly affect herbage production, but the inclusion of white clover in mixtures increased the yield of grasses (P〈0.01) The use of rosette-type forb blends increased forb yield (P〈0.01), compared with erect-type blends.The effects of treatments on herbage N and mineral contents and yields were inconsistent. However, there was some evidence to support the view that the presence of forb species in swards can result in greater contents of minerals in herbage, compared with grass-only swards.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 36 (1981), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The digestibility of herbage selected by dairy cows, milk-fed calves, weaned calves and wether sheep was examined on four occasions each day as they strip-grazed together a daily allocation of herbage. The average quality of the material selected by each group was similar. Organic matter digestibility of the herbage selected declined from 0·274 to 0·223 as the average height of the sward fell from 16 to 6 cm; this decline was slightly more rapid for the cows than for the other stock. A similar trend was also evident in samples cut from the sward to simulate the horizon removed prior to each sampling time.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 36 (1981), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of severity of grazing on the herbage intake and milk production of continuously stocked British Friesian cows calving in February–March were examined in three experiments conducted in the years 1976–78 (experiments 1–3 respectively) using a put-and-take technique. In experiment 1 four grazing severities were imposed by maintaining swards with different herbage masses (2500, 3000, 3500, 4000 kg OM ha-1); in experiments 2 and 3 there were two severities of grazing maintained by keeping swards canopies at constant heights of 5 and 7 cm (experiment 2) and 5 and 7·2 cm (experiment 3). Cows were reallocated to treatment every 8 weeks in experiments 1 and 2 and there were three periods, whereas they all grazed throughout a 23-week period on the same treatment in the final trial.A decrease in the quantity of herbage on offer or in sward height reduced herbage intake and milk production in all experiments. Mean daily herbage OM intakes were 11·2, 12·2, 12·2 and 12·2 kg respectively in experiment 1, 12·2 and 13·2 kg respectively in experiment 2 and 12·2 and 152 kg respectively in experiment 3. Mean daily solids–corrected milk yields were 14·2, 15·2, 15·2 and 16·2 kg respectively in experiment 1, 14·2 and 16·2 kg respectively in experiment 2 and 12·2 and 17·2 kg respectively in experiment 3. It was apparent from the data obtained in the first two trials that grazing at a sward canopy height of 7 rather than 9 cm had little effect, but that at 5 cm there were significant depressions in both herbage intake and milk production. Milk yield was depressed to a greater extent when cows were kept on the same treatment for the whole season.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 36 (1981), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Twenty-four Hereford × Friesian cows and their South Devon cross calves were allocated to three herbage allowances allotted daily for three periods of 8 weeks in a Latin square design. The daily allowances were 17, 34 and 51 g dry matter per kg cow plus calf live weight. Milk production was depressed by 0·2 and 1·2 kg d-1 at the medium and low allowances. The corresponding falls in liveweight gain were for cows 0·26 and 0·25, and for calves 0·27 and 0·24 kg d-1. Residual sward height after grazing gave a better indication of the animals' reaction to sward conditions and the management imposed than actual herbage allowance. The quantity per unit area and the composition of material present were important factors influencing intake. Calves were unable to compete with their dams to maintain herbage intake at the lower allowances and therefore are likely to benefit from additional feeding or creep grazing when residual sward height falls below 6cm for periods in excess of 1–2 weeks.
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