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  • RAPD  (40)
  • Springer  (40)
  • American Chemical Society
  • 1995-1999  (40)
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  • Springer  (40)
  • American Chemical Society
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  • 1
    Electronic Resource
    Electronic Resource
    A sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii[Mirb.] Franco var ‘menziesii’) based on RFLP and RAPD markers (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 762-770 
    Details
    ISSN: 1432-2242
    Keywords: Key words RFLP ; RAPD ; Genetic map ; Null loci ; Gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have constructed a sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco var ‘menziesii’) using a three-generation outcrossed pedigree and molecular markers. Our research objectives are to learn about genome organization and to identify markers associated with adaptive traits. The map reported here is comprised of 141 markers organized into 17 linkage groups and covers 1,062 centiMorgans (cM). Of the markers positioned on the map, 94 were derived from a Douglas-fir complimentary-DNA (cDNA) library that was constructed from new-growth needle tissue. Other markers include 11 Douglas-fir genomic-DNAs, 20 loblolly pine (Pinus taeda L.) cDNAs, 15 random amplified polymorphic DNAs (RAPDs) and a PCR-amplified phytochrome probe. A high degree of variation was detected in each of the two parents of our mapping population, and many of the restriction fragment length polymorphism (RFLP) and RAPD phenotypes were complex. Marker data were analyzed for linkage using mapping software JOINMAP version 2.0.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050953
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  • 2
    Electronic Resource
    Electronic Resource
    The use of RAPD for verifying the apomictic status of seedlings of Malus species (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1080-1083 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsMalus ; Apomixis ; RAPD ; Baskatong ; Red-purple pigmentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The lack of red-purple pigmentation in seedlings obtained from crosses between M. cv Baskatong, carrying a dominant homozygous gene for red-purple pigmentation, and other species has been used for the detection of apomictic plants in Malus species. RAPD marker techniques were employed to evaluate the genetic similarity between putative apomictic seedlings and their female parents. From the selected set of 20 (OPA) primers about half were able to detect hybrids from the apomictic seedlings, if present. RAPD analyses confirmed the usefulness of the colour-marker gene in detecting the hybrids in vitro for seedlings of M. toringoides×M. cv Baskatong, but not for crosses involving M. hupehensis×M. cv Baskatong where in vitro colour-based selection was not possible (due to red stems in all cases). The set of primers (OPA-01, 02, 08, 09, 10, 12, 13, 14, 16, 18 and 20) clearly determined the hybrid nature of seedlings and allowed the selection of apomictic ones. Therefore, although cv Baskatong is useful as an indicator, these data show that this technique is not applicable in all cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050665
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  • 3
    Electronic Resource
    Electronic Resource
    A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 673-679 
    Details
    ISSN: 1432-2242
    Keywords: Pinus radiata ; Genetic linkage map ; RFLP ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic linkage map for radiata pine (Pinus radiata D. Don) has been constructed using segregation data from a three-generation outbred pedigree. A total of 208 loci were analyzed including 165 restriction fragment length polymorphism (RFLP), 41 random amplified polymorphic DNA (RAPD) and 2 microsatellite markers. The markers were assembled into 22 linkage groups of 2 or more loci and covered a total distance of 1382 cM. Thirteen loci were unlinked to any other marker. Of the RFLP loci that were mapped, 93 were detected by loblolly pine (P. taeda L.) cDNA probes that had been previously mapped or evaluated in that species. The remaining 72 RFLP loci were detected by radiata pine probes from a PstI genomic DNA library. Two hundred and eighty RAPD primers were evaluated, and 41 loci which were segregating in a 1∶1 ratio were mapped. Two microsatellite markers were also placed on the map. This map and the markers derived from it will have wide applicability to genetic studies in P. radiata and other pine species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226088
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  • 4
    Electronic Resource
    Electronic Resource
    Comparison of four molecular markers in measuring relationships among the wild potato relatives Solanum section Etuberosum (subgenus Potatoe) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 532-540 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Genetic distance ; Potato ; RAPD ; RFLP ; Solanum section Etuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   We evaluated chloroplast DNA (cpDNA), isozymes, single to low-copy nuclear DNA (RFLPs), and random amplified polymorphic DNAs (RAPDs) in terms of concordance for genetic distance of 15 accessions each of Solanum etuberosum and S. palustre, and 4 accessions of S. fernandezianum. These self-compatible, diploid (2n=24), and morphologically very similar taxa constitute all species in Solanum sect. Etuberosum, a group of non-tuber-bearing species closely related to Solanum sect. Petota (the potato and its wild relatives). Genetic distance and multidimentional scaling results show general concordance of isozymes, RFLPs and RAPDs between all three taxa; cpDNA shows S. etuberosum and S. palustre to be more similar to each other than to S. fernandezianum. Interspecific sampling variance shows a gradation of resolution from allozyme (low) to RAPD to RFLP (high); while intraspecific comparisons graded from RFLPs (low) to RAPDs (high; lack of sufficient allozyme variability within species precluded comparisons for allozymes). Experimental error was low in RFLPs and RAPDs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050160
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of four molecular markers in measuring relationships among the wild potato relatives Solanum section Etuberosum (subgenus Potatoe) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 532-540 
    Details
    ISSN: 1432-2242
    Keywords: Genetic distance ; Potato ; RAPD ; RFLP ; Solanum section Etuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We evaluated chloroplast DNA (cpDNA), isozymes, single to low-copy nuclear DNA (RFLPs), and random amplified polymorphic DNAs (RAPDs) in terms of concordance for genetic distance of 15 accessions each of Solanum etuberosum and S. palustre, and 4 accessions of S. fernandezianum. These self-compatible, diploid (2n=24), and morphologically very similar taxa constitute all species in Solanum sect. Etuberosum, a group of non-tuber-bearing species closely related to Solanum sect. Petota (the potato and its wild relatives). Genetic distance and multidimentional scaling results show general concordance of isozymes, RFLPs and RAPDs between all three taxa; cpDNA shows S. etuberosum and S. palustre to be more similar to each other than to S. fernandezianum. Interspecific sampling variance shows a gradation of resolution from allozyme (low) to RAPD to RFLP (high); while intraspecific comparisons graded from RFLPs (low) to RAPDs (high; lack of sufficient allozyme variability within species precluded comparisons for allozymes). Experimental error was low in RFLPs and RAPDs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224555
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  • 6
    Electronic Resource
    Electronic Resource
    Mapping the Sw-5 locus for tomato spotted wilt virus resistance in tomatoes using RAPD and RFLP analyses (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 451-456 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; Lycopersicon peruvianum ; RAPD ; RFLP ; Tomato spotted wiltvirus (TSWV)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Sw-5 locus confers dominant resistance to tomato spotted wilt virus (TSWV). To map the location and facilitate the identification of markers linked to Sw-5 we developed a pair of near-isogenic lines (NILs) and an F2 Lycopersicon esculentum x L. pennellii population segregating for resistance to TSWV. DNA from the NILs was analyzed using 748 random 10-mer oligonucleotides to discern linked molecular markers using a random amplified polymorphic DNA (RAPD) approach. One random primer (GAGCACGGGA) was found to produce a RAPD band of about 2200 bp that demonstrates linkage to Sw-5. Data from co-segregation of resistance and restriction fragment length polymorphisms (RFLPs) in a F2 interspecific population position Sw-5 between the markers CT71 and CT220 near the telomere of the long arm of chromosome 9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221989
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  • 7
    Electronic Resource
    Electronic Resource
    A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 673-679 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pinus radiata ; Genetic linkage map ; RFLP ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A genetic linkage map for radiata pine (Pinus radiata D. Don) has been constructed using segregation data from a three-generation outbred pedigree. A total of 208 loci were analyzed including 165 restriction fragment length polymorphism (RFLP), 41 random amplified polymorphic DNA (RAPD) and 2 microsatellite markers. The markers were assembled into 22 linkage groups of 2 or more loci and covered a total distance of 1382 cM. Thirteen loci were unlinked to any other marker. Of the RFLP loci that were mapped, 93 were detected by loblolly pine (P. taeda L.) cDNA probes that had been previously mapped or evaluated in that species. The remaining 72 RFLP loci were detected by radiata pine probes from a PstI genomic DNA library. Two hundred and eighty RAPD primers were evaluated, and 41 loci which were segregating in a 1:1 ratio were mapped. Two microsatellite markers were also placed on the map. This map and the markers derived from it will have wide applicability to genetic studies in P. radiata and other pine species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050178
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic diversity of the ectomycorrhizal fungus Pisolithus tinctorius based on RAPD-PCR analysis (1998)
    Springer
    Mycorrhiza 7 (1998), S. 243-248 
    Details
    ISSN: 1432-1890
    Keywords: Key words DNA polymorphism ; Ectomycorrhizal fungi ; Genetic diversity ; Pisolithus tinctorius ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Twenty Pisolithus tinctorius isolates from different geographic locations and different hosts were characterized by the random amplified polymorphic DNA technique. Thirteen arbitrary primers generated 87 DNA fragments, all of them polymorphic. These data were used to calculate genetic distances among the isolates. The pairwise genetic distances ranged from 1 to 100%, with an average of 58.7%. Cluster analysis based on the amplified fragments grouped the isolates according to their host and geographical origins. Group I contained isolates collected in Brazil and group II those collected in the Northern Hemisphere. In addition to the diversity seen at the molecular level, the isolates also showed host specificity. Greenhouse experiments demonstrated that isolates from the Northern Hemisphere colonized mainly Pinus whereas isolates from Brazil colonized only Eucalyptus. The molecular data suggest that the Pisolithus tinctorius isolates analyzed belong to two distinct groups. The data also suggest new guidelines for future investigations on the taxonomy and systematic of this important fungus species. Furthermore, these results support future experiments aimed at the selection and development of improved isolates of P. tinctorius.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720050187
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  • 9
    Electronic Resource
    Electronic Resource
    DNA Isolation and AFLP™ Genetic Fingerprinting of shape Theobroma cacao (L.) (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 49-59 
    Details
    ISSN: 1572-9818
    Keywords: amplified fragment length polymorphism ; cocoa ; RAPD ; woody plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Accurate identification of parental plants and their hybrids is essential for an effective breeding programme. Traditional classification of cocoa varieties relies on the characterisation of agricultural traits at plant maturity. A rapid and reliable method is described, based on genotypic analysis. An efficient DNA isolation procedure was developed, yielding unsheared DNA of high purity. Two genetic fingerprinting techniques, RAPD and AFLP™, were evaluated for their suitability in distinguishing cocoa varieties. RAPD analysis was unsatisfactory due to the low frequency of polymorphisms and poor reproducibility. AFLP™ was reliable in distinguishing phenotypically identical, known varieties of cocoa. Importantly, AFLP™ also revealed intra- and inter-varietal variation. Abbreviations: AFLP™, amplified fragment length polymorphism; APS, ammonium persulphate; CTAB, hexadecyltrimethylammonium bromide; DEB, DNA extraction buffer; f.wt., fresh weight; NEB, nuclei extraction buffer; PMSF, phenylmethanesulphonyl fluoride; RAPD, random amplified polymorphic DNA; T4 PNK, Bacteriophage T4 polynucleotide kinase; Taq, Thermus aquaticus; TBE, tris-borate-EDTA; TEMED, NNN′N′ tetramethylethylenediamine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007468624596
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  • 10
    Electronic Resource
    Electronic Resource
    Somaclonal variation in cryopreserved embryogenic clones of white spruce [Picea glauca (Moench) Voss.] (1999)
    Springer
    Plant cell reports 18 (1999), S. 948-953 
    Details
    ISSN: 1432-203X
    Keywords: Key words Somaclonal variation ; Picea glauca ; RAPD ; Somatic embryogenesis ; Cryopreservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Trees were regenerated from six white spruce embryogenic clones after cryopreservation for 3 and 4 years, respectively. Genetic stability was evaluated using randomly amplified polymorphic DNA (RAPD) fingerprints. Somaclonal variation was detected in some in vitro embryogenic cultures 2 and 12 months after they were re-established following cryopreservation but not in the corresponding regenerated trees. These results suggest that trees regenerated from cryopreserved cultures in subsequent years are primarily genetically stable in the genomic regions tested and that variation observed due to the in vitro culture process infrequently affects trees regenerated from normally maturing and germinating somatic embryos. However, trees regenerated from somatic embryos that matured or germinated abnormally in in vitro culture exhibited altered RAPD fragment patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050689
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